Enhanced adenosine triphosphate production by Saccharomyces cerevisiae using an efficient energy regeneration system

Abstract

The process of ATP biosynthesis from adenosine catalyzed by Saccharomyces cerevisiae was studied using an efficient energy regeneration system. A fractional factorial design (2 9-5 ) was used to evaluate the effects of different components in the medium. Magnesium chloride, toluene, and acetaldehyde were found to significantly influence ATP production. The concentrations of the three factors were then optimized using central composition design and response surface analysis. Based on the second-order polynomial model obtained from the experiments, the optimal parameters were obtained as follows: adenosine 20 g/L; glucose 67 g/L; S. cerevisiae cells 250 g/L; magnesium chloride 4.37 g/L; potassium dihydrogen phosphate 67 g/L; toluene 1.40 mL/L; acetaldehyde 2.67 mL/L; pH 7.0; and temperature 37.0 o C. Under the condition, the yield and concentration of ATP reached 97.5% and 37 g/L, respectively. The yield was nearly 10% higher than the level before optimization and the concentration increased two-fold. In addition, the utilization efficiency of energy after optimization increased nearly 6%.

In vitro biosynthesis of ATP from adenosine and polyphosphate

“…In this process, glucose is added to provide energy for the ATP production process through the glycolytic pathway. Nevertheless, the energy utilization of this method is low, and the yeast enzyme system with different batches exhibits large differences in productivity (Yao et al 2011 ). ATP has been used as medicine and for industrial biosynthesis of costly medicine precursors, but its market price of about 150 US dollars per kilogram remains too costly.…”

Phosphorus sustainability: a case for phytic acid as a biorenewable platform

“…55 ATP is manufactured for industrial use using yeast cells, 56 a process with poor energy efficiency. 57 Isolation of ATP from the fermentation broth necessitates multiple steps, long processing times (days to weeks), and often contaminants such as adenosine diphosphate (ADP) and adenosine monophosphate remain present in large quantities. 58 To combat these inefficiencies, chromatographic isolation processes for ATP on cryogel columns have been developed, 58,59 although the industrial applicability of these methods remains unclear.…”

Nucleoside Analogs: A Review of Its Source and Separation Processes

“…Takashi Tsuji et al [68] proposed a biotransformation method for producing 2 -deoxyadenosine, which uses 2deoxythymidine as a ribose group donor and adenine as a base donor. Ying groups [69] synthesized adenosine triphosphate using the yeast Saccharomyces cerevisiae while study-ing the various factors that influence the yield. Francesca Paradisi et al [70] successfully synthesized four nucleoside analogs: 5-fluoro-2 -deoxyuridine, 5-chloro-2 -deoxyuridine, 5-bromo-2 -deoxyuridine, and 5-iodo-2 -deoxyuridine, using a newly discovered thymidine phosphorylase, and the conversion rate reached 90% at a concentration of 10 mM (Figure 4).…”