Enhanced adhesion and proliferation of human umbilical vein endothelial cells on conductive PANI-PCL fiber scaffold by electrical stimulation

Li, Yumei; Li, Xiang; Zhao, Rui; Wang, Chuying; Fang-ping, Qiu; Sun, Bolun; Ji, He; Qiu, Jianying; Wang, Ce

doi:10.1016/j.msec.2016.11.052

Cited by 84 publications

(46 citation statements)

References 39 publications

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“…Ghasemi-Mobarakeh et al reported that applying direct current to polyaniline/gelatin (15:85) with electrical conductivity of 0.02 × 10 −6 S amended the NSCs proliferation and neurite outgrowth; 24 likewise, Li et al used polyaniline/PCL nanofibers with electrical stimulation to culture human umbilical vein endothelial cells (HUVECs) and reported promotion in growth for the cultured cells in the presence of conductive polymer. 25 As shown in Figure 2C, the electrical conductivity of nanofiber scaffolds was PAG dose-dependent. In comparison to the results reported by Baniasadi et al, the conductivity of the fabricated nanofiber scaffolds in the present study declined due to the presence of PCL nanofibers although its conductivity is around 1.2×10 −5 S/cm which is still in an appropriate range to be used for neural tissue engineering.…”

Section: Electrical Conductivity Measurementmentioning

confidence: 83%

<p>Bioinspired Nanofiber Scaffold for Differentiating Bone Marrow-Derived Neural Stem Cells to Oligodendrocyte-Like Cells: Design, Fabrication, and Characterization</p>

Boroojeni¹,

Mashayekhan²,

Abbaszadeh³

et al. 2020

IJN

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Background: Researchers are trying to study the mechanism of neural stem cells (NSCs) differentiation to oligodendrocyte-like cells (OLCs) as well as to enhance the selective differentiation of NSCs to oligodendrocytes. However, the limitation in nerve tissue accessibility to isolate the NSCs as well as their differentiation toward oligodendrocytes is still challenging. Purpose: In the present study, a hybrid polycaprolactone (PCL)-gelatin nanofiber scaffold mimicking the native extracellular matrix and axon morphology to direct the differentiation of bone marrow-derived NSCs to OLCs was introduced. Materials and Methods: In order to achieve a sustained release of T3, this factor was encapsulated within chitosan nanoparticles and chitosan-loaded T3 was incorporated within PCL nanofibers. Polyaniline graphene (PAG) nanocomposite was incorporated within gelatin nanofibers to endow the scaffold with conductive properties, which resemble the conductive behavior of axons. Biodegradation, water contact angle measurements, and scanning electron microscopy (SEM) observations as well as conductivity tests were used to evaluate the properties of the prepared scaffold. The concentration of PAG and T3-loaded chitosan NPs in nanofibers were optimized by examining the proliferation of cultured bone marrow-derived mesenchymal stem cells (BMSCs) on the scaffolds. The differentiation of BMSCs-derived NSCs cultured on the fabricated scaffolds into OLCs was analyzed by evaluating the expression of oligodendrocyte markers using immunofluorescence (ICC), RT-PCR and flowcytometric assays. Results: Incorporating 2% PAG proved to have superior cell support and proliferation while guaranteeing electrical conductivity of 10.8 × 10 −5 S/cm. Moreover, the scaffold containing 2% of T3-loaded chitosan NPs was considered to be the most biocompatible samples. Result of ICC, RT-PCR and flow cytometry showed high expression of O4, Olig2, platelet-derived growth factor receptor-alpha (PDGFR-α), O1, myelin/oligodendrocyte glycoprotein (MOG) and myelin basic protein (MBP) high expressed but low expression of glial fibrillary acidic protein (GFAP). Conclusion: Considering surface topography, biocompatibility, electrical conductivity and gene expression, the hybrid PCL/gelatin scaffold with the controlled release of T3 may be considered as a promising candidate to be used as an in vitro model to study patient-derived oligodendrocytes by isolating patient's BMSCs in pathological conditions such as diseases or injuries. Moreover, the resulted oligodendrocytes can be used as a desirable source for transplanting in patients.

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Section: Electrical Conductivity Measurementmentioning

confidence: 83%

<p>Bioinspired Nanofiber Scaffold for Differentiating Bone Marrow-Derived Neural Stem Cells to Oligodendrocyte-Like Cells: Design, Fabrication, and Characterization</p>

Boroojeni¹,

Mashayekhan²,

Abbaszadeh³

et al. 2020

IJN

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“…found that biocompatibility of PANI can be increased by repeated deprotonation and reprotonation cycles, indicating that the release of by-products of the polymer synthesis is the main cause of cytotoxicity of PANI rather than PANI itself [32]. This study assessed cell proliferation up to 21 days, which is longer than other studies [26,32,54,78] and may allow the build-up of cytotoxic effects that might not be observed during shorter studies. Therefore, further investigation is required to ascertain the role of dopant or by-product release on biocompatibility and additional purification and washing steps of the samples may be required.…”

Section: Cell Viability and Proliferationmentioning

confidence: 97%

3D Printing of Polycaprolactone–Polyaniline Electroactive Scaffolds for Bone Tissue Engineering

et al. 2020

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Electrostimulation and electroactive scaffolds can positively influence and guide cellular behaviour and thus has been garnering interest as a key tissue engineering strategy. The development of conducting polymers such as polyaniline enables the fabrication of conductive polymeric composite scaffolds. In this study, we report on the initial development of a polycaprolactone scaffold incorporating different weight loadings of a polyaniline microparticle filler. The scaffolds are fabricated using screw-assisted extrusion-based 3D printing and are characterised for their morphological, mechanical, conductivity, and preliminary biological properties. The conductivity of the polycaprolactone scaffolds increases with the inclusion of polyaniline. The in vitro cytocompatibility of the scaffolds was assessed using human adipose-derived stem cells to determine cell viability and proliferation up to 21 days. A cytotoxicity threshold was reached at 1% wt. polyaniline loading. Scaffolds with 0.1% wt. polyaniline showed suitable compressive strength (6.45 ± 0.16 MPa) and conductivity (2.46 ± 0.65 × 10−4 S/cm) for bone tissue engineering applications and demonstrated the highest cell viability at day 1 (88%) with cytocompatibility for up to 21 days in cell culture.

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“…The abundant quinoid imine (-N=) enhances the electron delocalization, ensuring high conductivity of PANI chains. [46][47][48] Besides, the peak appearing at 208 eV in the full spectrum is ascribed to an enriched doping of ClO 4 − in PANI, which results in numerous unsaturated bonds and provides substantial reactive sites for the redox reaction [49]. Accordingly, RGO/PEDOT/PANI exhibits a higher capacitance (535 F g −1 ) compared with RGO/PEDOT (228 F g −1 ).…”

Section: Go/pedot:pss Rgo/pedot Rgo/pedot/pani Go/pedot:pss Rgo/pedotmentioning

confidence: 99%

Hierarchical Porous RGO/PEDOT/PANI Hybrid for Planar/Linear Supercapacitor with Outstanding Flexibility and Stability

et al. 2020

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HIGHLIGHTS • Hierarchical porous reduced graphene oxide/poly(3,4-ethylenedioxythiophene)/polyaniline hybrid was designed. • The hybrid achieves a high capacitance of 535 F g −1 along with a good rate capability and cyclability. ABSTRACT Many hybrid electrodes for supercapacitors (SCs) are a reckless combination without proper structural design that keeps them from fulfilling their potential. Herein, we design a reduced graphene oxide/poly(3,4-ethylenedioxythiophene)/polyaniline (RGO/PEDOT/PANI) hybrid with hierarchical and porous structure for high-performance SCs, where components fully harness their advantages, forming an interconnected and conductive framework with substantial reactive sites.Thus, this hybrid achieves a high capacitance of 535 F g −1 along with good rate capability and cyclability. The planar SC based on this hybrid deliver an energy density of 26.89 Wh kg −1 at a power density of 800 W kg −1. The linear SC developed via modifying a cotton yarn with the hybrid exhibits good flexibility and structural stability, which operates normally after arbitrary deformations. This work provides a beneficial reference for developing SCs.

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Enhanced adhesion and proliferation of human umbilical vein endothelial cells on conductive PANI-PCL fiber scaffold by electrical stimulation

Cited by 84 publications

References 39 publications

<p>Bioinspired Nanofiber Scaffold for Differentiating Bone Marrow-Derived Neural Stem Cells to Oligodendrocyte-Like Cells: Design, Fabrication, and Characterization</p>

<p>Bioinspired Nanofiber Scaffold for Differentiating Bone Marrow-Derived Neural Stem Cells to Oligodendrocyte-Like Cells: Design, Fabrication, and Characterization</p>

3D Printing of Polycaprolactone–Polyaniline Electroactive Scaffolds for Bone Tissue Engineering

Hierarchical Porous RGO/PEDOT/PANI Hybrid for Planar/Linear Supercapacitor with Outstanding Flexibility and Stability

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