Enhanced and Specific Gene Expression via Tissue-Specific Production of Cre Recombinase Using Adenovirus Vector

Sato, Yuichi; Tanaka, Keiji; Lee, Gwang; Kanegae, Yumi; Sakai, Yoshio; Kaneko, Shuichi; Nakabayashi, Hidekazu; Tamaoki, Taiki; Saito, Izumu

doi:10.1006/bbrc.1997.8087

Cited by 90 publications

(88 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several specific promoters to cancers such as ␣-fetoprotein (AFP) 62,63 and carcinoembryonic antigen 64,65 have been reported to be valuable, but the expression levels of these promoters are relatively low. To improve the expression level of the AFP promoter, Sato et al 66 used the Cre/loxP system and demonstrated that this double infection method resulted in ϳ50-fold higher expression compared with a single Adv infection directly driven by the AFP promoter. However, even though this Cre/loxP system produces a higher expression level compared with that of the conventional system, some cytotoxic genes such as Fas ligand, which is controlled by the MBP promoter, do not induce cell death effectively in gliomas (data not shown), whereas those genes controlled by the CA promoter can.…”

Section: Discussionmentioning

confidence: 99%

Adenovirus-mediated transfer of Bax with caspase-8 controlled by myelin basic protein promoter exerts an enhanced cytotoxic effect in gliomas

et al. 2000

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Adenovirus-mediated transfer of Bax with caspase-8 controlled by myelin basic protein promoter exerts an enhanced cytotoxic effect in gliomas

et al. 2000

View full text Add to dashboard Cite

“…35 Cosmid pAxCALNLmBMP-7 was constructed by the insertion of mouse BMP-7 cDNA into the SwaI cloning site of pAxCALNLw. 36 By the COS-TPC method, 37 recombinant adenovirus of AxCALNLmBMP-7 was generated by transfecting 293 cells with the pAxCALNLSmad7. AxCANCre was generated by transfecting 293 cells with Ax-CALNLCreDNA-TPC as described in the manufacturer's protocol.…”

Section: Adenovirus Vector Construction and Virus Purificationmentioning

confidence: 99%

Therapeutic effects of adenoviral gene transfer of bone morphogenic protein-7 on a corneal alkali injury model in mice

Saika

Ikeda

Yamanaka

et al. 2005

Laboratory Investigation

View full text Add to dashboard Cite

An alkali burn in the cornea is a common serious clinical problem often leading to permanent visual impairment. Since transforming growth factor-b (TGF-b) is involved in the response to corneal injury, we evaluated the therapeutic effects of adenoviral gene transfer of mouse bone morphogenic proten-7 (BMP-7), which has antagonistic effects on TGF-b in tissue fibrosis. Burned cornea did not express endogenous BMP-7 mRNA and protein. Resurfacing of the burned cornea by invading conjunctival epithelium was accelerated by adenoviral introduction of BMP-7. Exogenous BMP-7 expression also suppressed myofibroblast generation, appearance of monocytes/macrophages and expression of MCP-1, TGF-bs, and collagen I a2 chain in the affected stroma. Ectopic BMP-7 did not suppress stromal neovascularization throughout the interval studied and also did not reduce VEGF mRNA expression at Day 10. Ectopic BMP-7 in burned corneal tissue resulted in activation of Smad1/5/8 signaling and partial suppression of the phospho-Smad2 signal. These data suggest that overexpression of BMP-7 is an effective strategy for treatment of ocular alkali burns.

show abstract

“…34 The CreloxP system has been introduced into adenovirus -mediated gene therapy in order to enhance cell killing ability without losing tissue specificity. 35 Nagayama et al 36 tried this system for experimental gene therapy for thyroid carcinomas using HSVtk genes as a suicide gene driven by a TG promoter. By using stable transfectants but not infection with viruses, Kitazono et al 37 reported that the enhancer sequence of the TG promoter increased transcriptional activity in combination treatment with histone deacetylase inhibitor or sodium butyrate in vitro.…”

mentioning

confidence: 99%

A tandemly repeated thyroglobulin core promoter has potential to enhance efficacy for tissue-specific gene therapy for thyroid carcinomas

et al. 2002

View full text Add to dashboard Cite

Recombinant adenoviruses, carrying herpes simplex virus thymidine kinase ( HSVtk ) genes, were developed to evaluate the possibility of tissue -specific gene therapy for thyroid carcinomas. The HSVtk gene was driven by a minimal thyroglobulin ( TG ) promoter ( AdTGtk ) and a tandemly repeated minimal TG promoter ( Ad2ÂTGtk ) to obtain thyroid -specific cell killing ability. The transduction of HSVtk genes by infection with Ad2ÂTGtk followed by ganciclovir ( GCV ) treatment showed more powerful cytotoxicity for TG -producing FRTL5 cells, a rat normal thyroid cell line, and FTC -133 cells, a human follicular thyroid carcinoma cell line, than when infected with AdTGtk in vitro. The cell killing ability of Ad2ÂTGtk was 10 -to 30 -fold higher than that of AdTGtk and similar to that of AdCMVtk, which carries HSVtk under the control of CMV promoter. Whereas after treatment with adenovirus / GCV to non -TG -producing cell lines ( undifferentiated thyroid carcinoma cell lines and carcinoma cell lines from other tissues ), Ad2ÂTGtk and AdTGtk needed more than 100 -fold concentrated GCV to reach IC 50 compared to AdCMVtk. We confirmed the enhanced efficacy of Ad2ÂTGtk for tissue -specific cytotoxicity in vivo. After adenovirus / GCV treatment for FTC -133 tumorbearing nude mice, Ad2ÂTGtk enhanced tumor growth inhibition and survival rates compared to AdTGtk. Tumor growth inhibition and survival rates by Ad2ÂTGtk were similar to that by AdCMVtk. Moreover, any toxic effect for rat normal tissues was not revealed after intravenous injections with Ad2ÂTGtk and intraperitoneal administrations with GCV in vivo, whereas severe liver damages were observed after treatment with AdCMVtk / GCV. These data indicate a beneficial effect of Ad2ÂTGtk for tissue -specific gene therapy for TG -producing thyroid carcinomas without toxicity for normal tissues.

show abstract

Enhanced and Specific Gene Expression via Tissue-Specific Production of Cre Recombinase Using Adenovirus Vector

Cited by 90 publications

References 26 publications

Adenovirus-mediated transfer of Bax with caspase-8 controlled by myelin basic protein promoter exerts an enhanced cytotoxic effect in gliomas

Adenovirus-mediated transfer of Bax with caspase-8 controlled by myelin basic protein promoter exerts an enhanced cytotoxic effect in gliomas

Therapeutic effects of adenoviral gene transfer of bone morphogenic protein-7 on a corneal alkali injury model in mice

A tandemly repeated thyroglobulin core promoter has potential to enhance efficacy for tissue-specific gene therapy for thyroid carcinomas

Contact Info

Product

Resources

About