Enhanced and stabilized mesenchymal stem cell growth inside plasma pre-treated and collagen-coated PDMS-based microfluidic chambers

Kefallinou, Dionysia; Grigoriou, Maria; Constantoudis, Vassilios; Raptis, Ioannis; Xenogiannopoulou, Evangelia; Boumpas, Dimitrios T.; Tserepi, Angeliki

doi:10.1016/j.surfcoat.2023.129658

Cited by 12 publications

(6 citation statements)

References 57 publications

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“…The deconvoluted C1s spectra for particular samples are introduced in Figure 5. As it is obvious from the deconvoluted spectra, the results are in good agreement with those observed by Keffalinou et al [90], although some particular differences are likely attributed to the type of applied plasma, where we used an argon inert treatment. It is obvious, that after collagen coating, there is no significant difference between both deconvoluted spectra (Figure 5B,D), but the difference is visible compared with the uncoated samples (Figure 5A,C).…”

Section: Study Of Surface Chemistrysupporting

confidence: 90%

“…We also determined the contact angle of selected samples. The pristine microstructure foil had a contact angle of 109 • , the plasma-treated PDMS foil exhibited a contact angle of 24 • , and the plasma-treated PDMS microstructure with collagen had a contact angle of 54 • ; these results are also in good agreement with Keffalinou et al [90]. Wettability-patterned surfaces prepared by an Atmospheric-Pressure Plasma Jet were used in microfluidic devices [91], and wettability also has a significant impact on microchannels in enhancing droplet coalescence and demulsification [92] and surface activation of polymer blends for recyclability [93].…”

Section: Study Of Surface Chemistrysupporting

confidence: 88%

“…Cells on PDMS micropattern with collagen on the sixth day from seeding were well spread and aligned along the pattern. Similar studies were performed with microfluidic chambers by Keffalinou et al [ 90 ]. The use of plasma pre-treated and collagen-coated PDMS-based microfluidic chambers has been able to enhance and stabilize mesenchymal stem cell growth inside.…”

Section: Resultssupporting

confidence: 55%

“…Microfluidic applications [67,68] PE CVD Applications that require stable hydrophilic surface property in PDMS [69] Photografting of PEG Protein adsorption and cell adhesion [71] Imidazolium antimicrobial compound Catheter fabrication [73] UV-light curing Drug delivery [75] Soft lithography Replication of cardiovascular flow conditions [80] Bulk oxygen, nitrogen, or argon + collagen Adhesive free skin substitutes [82] Bulk plasma treatment and collagen Bone marrow-derived stromal cells [83] Hot embossing Photonic devices [85][86][87] Different bulk/ surface treatments Biomedical applications [88,89] Surface plasma treatment and collagen Mesenchymal stem cells [90] Different bulk/surface treatments Wettability and recyclability applications [91][92][93] Plasma/collagen treatment Tissue engineering-different cell types [94][95][96][97][98] Bulk modification Cell mechanobiology in muscle and nerve [99] The promising results of this study suggest the potential for reconstructing threedimensional vascularized tissue suitable for implantation. Photolithographic masks were designed to emulate the shape, size, and orientation of muscle fibers.…”

Section: Plasma Treatmentmentioning

confidence: 99%

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Plasma-Activated Polydimethylsiloxane Microstructured Pattern with Collagen for Improved Myoblast Cell Guidance

Slepičková Kasálková,

Juřicová,

Fajstavr

et al. 2024

IJMS

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We focused on polydimethylsiloxane (PDMS) as a substrate for replication, micropatterning, and construction of biologically active surfaces. The novelty of this study is based on the combination of the argon plasma exposure of a micropatterned PDMS scaffold, where the plasma served as a strong tool for subsequent grafting of collagen coatings and their application as cell growth scaffolds, where the standard was significantly exceeded. As part of the scaffold design, templates with a patterned microstructure of different dimensions (50 × 50, 50 × 20, and 30 × 30 μm2) were created by photolithography followed by pattern replication on a PDMS polymer substrate. Subsequently, the prepared microstructured PDMS replicas were coated with a type I collagen layer. The sample preparation was followed by the characterization of material surface properties using various analytical techniques, including scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), and X-ray photoelectron spectroscopy (XPS). To evaluate the biocompatibility of the produced samples, we conducted studies on the interactions between selected polymer replicas and micro- and nanostructures and mammalian cells. Specifically, we utilized mouse myoblasts (C2C12), and our results demonstrate that we achieved excellent cell alignment in conjunction with the development of a cytocompatible surface. Consequently, the outcomes of this research contribute to an enhanced comprehension of surface properties and interactions between structured polymers and mammalian cells. The use of periodic microstructures has the potential to advance the creation of novel materials and scaffolds in tissue engineering. These materials exhibit exceptional biocompatibility and possess the capacity to promote cell adhesion and growth.

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Section: Study Of Surface Chemistrysupporting

confidence: 90%

Section: Study Of Surface Chemistrysupporting

confidence: 88%

Section: Resultssupporting

confidence: 55%

Section: Plasma Treatmentmentioning

confidence: 99%

See 2 more Smart Citations

Plasma-Activated Polydimethylsiloxane Microstructured Pattern with Collagen for Improved Myoblast Cell Guidance

Slepičková Kasálková,

Juřicová,

Fajstavr

et al. 2024

IJMS

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“…Inappropriately, plasma-treated PDMS surfaces become more hydrophobic over time, mainly due to the migration of low-molecular-weight PDMS species from the bulk to the surface [24][25][26]. Additional chemical coatings such as poly(vinyl alcohol) (PVA) or salinization followed by type-I collagen (col1) have been used in an attempt to enhance cell adhesion and surface stability [27][28][29]. Furthermore, plasma also permits a permanent bond of two PDMS layers together or into another surface (e.g.…”

Section: Introductionmentioning

confidence: 99%

Renal proximal tubule-on-a-chip in PDMS: fabrication, functionalization, and RPTEC:HUVEC co-culture evaluation

Guimaraes,

Calori,

Stilhano

et al. 2024

Biofabrication

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“On-a-chip” technology advances the development of physiologically relevant organ-mimicking devices by integrating human cells into a three-dimensional microfluidic architecture that simulates the fluid dynamics of the target tissue. This approach also creates minimal functional units, enabling compartmentalized research on the effects of drugs on individual organ components. In this study, we describe the construction and evaluation of a convoluted renal proximal tubule-on-a-chip (PT-on-a-chip), which consists of a co-culture of Renal Proximal Tubule Epithelial Cells (RPTEC) and Human Umbilical Vein Endothelial Cells (HUVEC) in a polydimethylsiloxane (PDMS) microfluidic device fabricated using 3D printing and molding. Remarkably, the RPTEC:HUVEC co-culture displayed rapid cell adhesion within 30 min on microchannels functionalized with plasma, APTES, and type-I collagen, thus significantly reducing the necessary incubation time before initiating medium perfusion. In contrast, other functionalization methods such as plasma alone and plasma plus polyvinyl alcohol were effective only in promoting cell attachment to plannar surfaces. The PT-on-a-chip holds great promise as a valuable tool for assessing the nephrotoxic potential of new drug candidates, enhancing our understanding of drug interactions with co-cultured renal cells, and reducing the need for animal experimentation, promoting the safe and ethical development of new pharmaceuticals.

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Atmospheric Pressure Plasma Functionalization of Sealed PDMS Microfluidics: Application to Capillary Pumping and Enhanced Cell Growth

Zeniou,

Kefallinou,

Dimitrakellis

et al. 2024

ChemPlusChem

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Microfluidic devices serve as essential tools across diverse fields like medicine, biotechnology, and chemistry, enabling advancements in analytical techniques, point‐of‐care diagnostics, microfluidic cell cultures, and organ‐on‐chip models. While polymeric microfluidics are favoured for their cost‐effectiveness and ease of fabrication, their inherent hydrophobic properties necessitate surface functionalization, often post‐sealing. Here, we introduce a versatile apparatus for functionalizing sealed microfluidic devices using atmospheric plasma processing, with a focus on PDMS (polydimethylsiloxane) microfluidics. Through meticulous analysis of surface properties and capillary velocity, before and after plasma treatment, along with a comparison between vacuum and atmospheric plasma functionalization methods, we demonstrate the efficacy of our approach. Subsequent experimentation within 3D PDMS microfluidic chambers, combining atmospheric pressure plasma treatment with collagen coating to facilitate mesenchymal stem cells (MSCs) growth over five days, reveals enhanced initial cell adhesion and proliferation, highlighting the potential of our method for improving cell‐based applications within microfluidic systems.

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Enhanced and stabilized mesenchymal stem cell growth inside plasma pre-treated and collagen-coated PDMS-based microfluidic chambers

Cited by 12 publications

References 57 publications

Plasma-Activated Polydimethylsiloxane Microstructured Pattern with Collagen for Improved Myoblast Cell Guidance

Plasma-Activated Polydimethylsiloxane Microstructured Pattern with Collagen for Improved Myoblast Cell Guidance

Renal proximal tubule-on-a-chip in PDMS: fabrication, functionalization, and RPTEC:HUVEC co-culture evaluation

Atmospheric Pressure Plasma Functionalization of Sealed PDMS Microfluidics: Application to Capillary Pumping and Enhanced Cell Growth

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