Enhanced and suppressed phosphorus mineralization by Ca complexation: NMR and CD spectroscopy investigation

Ai, Chen; Zhu, Lingyang; Arai, Yuji

doi:10.1016/j.chemosphere.2023.138761

Cited by 2 publications

(11 citation statements)

References 31 publications

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“…Another study showed that the phytase first targeted P1,3 and formed D-and/or L-Ins(1,2,4,5,6)P5 at pH 6. 20 This experimental evidence suggests that the preferential phosphate involved in hydrolysis and in adsorption is different, and there should not be any competition between phytase and calcite against the same phosphate on phytic acid.…”

Section: ■ Materials and Methodsmentioning

confidence: 91%

“…Peak assignment and product analysis were conducted according to a previous study. 20 Results of calcitephytase and InsP6 (aq) (Figure S6) and calcite-InsP6 and phytase (aq) (Figure S7) still showed the first removal of P5 and predominant formation of Ins(1,2,3,4,6)P5. Whether phytase and calcite competed for the same phosphate group is therefore unknown, and it is not yet clear how this could contribute to the enhancement in phytase activity.…”

Section: ■ Materials and Methodsmentioning

confidence: 97%

“…According to a previous study, InsP5 was the predominant degradation product within the 1 h phytic acid degradation process by this particular phytase, and the following analysis therefore focused on the first hydrolysis product, while the lower-order species were not investigated. 20 To investigate if the phytase− or phytic acid−calcite interaction alters the reaction mechanism and generates different InsP5 products, 2D-TOCSY and selective 1D-TOCSY measurements were conducted on the following two systems: calcite-phytase and phytic acid (aq) and calcite-phytic acid and phytase (aq) (Figures S2−S4). To obtain stronger signals from the target InsP5 product so that the assignment can be made more accurately, a longer degradation time was allowed in the TOCSY measurements than in the kinetics experiment.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…At pH 8, phytase targeted P5 first, leading to the formation of Ins(1,2,3,4,6)P5 while releasing phosphate. 20 2D-TOCSY (Figure S5) and selective 1D-TOCSY were used to study the effects of calcite−phytic acid or calcite−phytase interaction on product formation. Peak assignment and product analysis were conducted according to a previous study.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…Chen et al showed that Ca 2+ (aq) affected phytase activity, and although similar effects may be expected from calcite, how phytase properties are influenced by mineral surfaces may be different from metals (aq). 20 For example, metal ions in solution alter the surface tension of water through their interaction with water. This, in turn, shifts the protein conformation toward a more folded or unfolded form, depending on how the surface tension is affected.…”

Section: ■ Introductionmentioning

confidence: 99%

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Spectroscopic Investigation of Phosphorus Mineralization as Affected by the Calcite–Water Interfacial Chemistry

Chen,

Zhu,

Han

et al. 2023

Environ. Sci. Technol.

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The mineralization and bioavailability of phytic acid, the predominant organic phosphorus (OP) species in many soils, have generally been rendered limited due to its interaction with soil minerals. In particularly calcareous and neutral to slightly alkaline soils, phytic acid is known to actively react with calcite, although how this interaction affects phytic acid mineralization is still unknown. This study, therefore, investigated the mechanisms regarding how the calcite–water interface influences phytic acid mineralization by phytase, at pHs 6 and 8 using in situ spectroscopic techniques including solution nuclear magnetic resonance and attenuated total reflection Fourier transform infrared spectroscopy. The findings indicated a pH-specific effect of the calcite–water interface. Inhibited phytase activity and thus impaired phytic acid mineralization were induced by calcite at pH 6, while the opposite effect was observed at pH 8. How the interaction between phytic acid and calcite and between phytase and calcite differed between the two pH values contributed to the pH-specific effect. The results demonstrate the importance of soil pH, enzyme–, and OP–clay mineral interactions in controlling the mineralization and transformation of OP and, consequently, the release of phosphate in soils. The findings can also provide implications for the management of calcite-rich and limed soils.

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Section: ■ Materials and Methodsmentioning

confidence: 91%

Section: ■ Materials and Methodsmentioning

confidence: 97%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Spectroscopic Investigation of Phosphorus Mineralization as Affected by the Calcite–Water Interfacial Chemistry

Chen,

Zhu,

Han

et al. 2023

Environ. Sci. Technol.

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A Review on the Driving Forces in the Formation of Bioactive Molecules-Loaded Prolamin-Based Particles

Chen,

Xiong,

2024

J. Agric. Food Chem.

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Prolamin-based particles loaded with bioactive molecules have attracted widespread attention from scientists due to their novel properties in chemistry, physics, and biology. In the self-assembly process of biopolymer-based nanocapsules, noncovalent interactions are the main driving forces for reducing bulk materials to the nanoscale and controlling the release of bioactive molecules. This article reviews the types of interaction forces, binding strength, binding active sites, molecular orientation, and binding affinity that affect the release profile of bioactive molecules during the preparation of protein stabilizer particles. Different preparation formulations, the use of different biopolymers, the inherent nature of the loaded bioactive molecules, and external factors (including pH, biopolymer concentration, temperature, salt, ultrasonication, and atmospheric cold plasma treatment) lead to different types and strengths of intra- and intermolecular interactions. Strategies, such as pH, ultrasonication, and atmospheric cold plasma, to change the protein conformation are key to improving the binding strength between proteins and bioactive substances or stabilizers. This review provides some guidance for scientists and technicians dedicated to improving loading efficiency, delaying release, enhancing colloidal stability, and exploring the binding behavior among proteins, stabilizers, and bioactive molecules.

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Enhanced and suppressed phosphorus mineralization by Ca complexation: NMR and CD spectroscopy investigation

Cited by 2 publications

References 31 publications

Spectroscopic Investigation of Phosphorus Mineralization as Affected by the Calcite–Water Interfacial Chemistry

Spectroscopic Investigation of Phosphorus Mineralization as Affected by the Calcite–Water Interfacial Chemistry

A Review on the Driving Forces in the Formation of Bioactive Molecules-Loaded Prolamin-Based Particles

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