Enhanced anti-apoptosis and gut epithelium protection function of acidic fibroblast growth factor after cancelling of its mitogenic activity

Abstract

These findings emphasize the importance of non-mitogenic effects of aFGF, and have implications for its therapeutic use in preventing apoptosis and other injuries in tissues and internal organs triggered by ischemia-reperfusion injury.

Muscle cell survival mediated by the transcriptional coactivators p300 and PCAF displays different requirements for acetyltransferase activity

“…Several reports have described antiapoptotic actions of FGF-1 in cardiac and vascular smooth muscle (4,8,9), as well as in other cell types (12,51), in part through receptorstimulated activation of the MAP kinases ERK-1 and -2 (4). In agreement with the hypothesis that local production of FGF-1 could account for some of the effects of p300 on muscle cell survival, we found that conditioned culture medium from cells expressing p300 wt but not p300 wt ϩDox increased myoblast viability and could activate ERK-1 and -2.…”

Expression of The Human Acidic Fibroblast Growth Factor in Transgenic Tomato and Safety Assessment of Transgenic Lines

“…The first series of media contained different concentrations of glucose (0, 1, 2 and 5 g/l) and the second series contained mannose (10,12,15,20 and 30 g/l) along with 5 g/l glucose.…”

“…It takes part in multiple biological processes, including embryonic development, cell growth, morphogenesis, angiogenesis and tissue repair. Acidic FGF has been examined as therapeutic protein in animal and clinical trials for skin wound and burn healing (44), wound healing of diabetic patients (62), angiogenesis (22,49), neurotrophic activity (30,64), strong anti-apoptotic function (14,68), gut epithelium protection (20), spinal cord injury repair (65). New lymph vessels growth as well as recovery of injured ischemic blood vessels is increased when using therapy with aFGF (29).…”

“…It is used also in pressure ulcer healing (18). There are scientific reports about modifications made in the primary structure of aFGF for elimination of its strong mitogenic activity (70), for prolongation of its half-life and increased stability (73) which facilitate its use as a therapeutic protein (20,28,37). The gene for haFGF was transiently expressed in Nicotiana benthamiana (38,43), soybean and Arabidopsis (43).…”

Protective Effect of Modified Human Acidic Fibroblast Growth Factor against Actinomycin D-Induced NRK52E Cells Apoptotic Death

“…Modification of the N-terminal of aFGF gene and elimination of N terminal residues 1-27 plus methionine substitution of leucine at the site of 27 yields a human acidic fibroblast growth factor mutant (haFGF27-154), that is, modified aFGF (MaFGF) [8,9]. It has been shows that when the N-side 1 to 27 amino-acid residue of aFGF is removed, its mitogenic activity is lost or obviously decrease, but its non-mitogenic effect still exists [9][10][11][12].…”