Enhanced anti-cancer activity by curcumin-loaded hydrogel nanoparticle derived aggregates on A549 lung adenocarcinoma cells

Teong, Benjamin; Lin, Chia-Yun; Chang, Shwu-Jen; Niu, Gregory Cheng-Chie; Yao, Chun‐Hsu; Chen, I-Fen; Kuo, Shyh-Ming

doi:10.1007/s10856-014-5357-3

Cited by 35 publications

(26 citation statements)

References 31 publications

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“…Moreover, natural compound HET was initially limited by its low solubility and bioavailability in cell-culturing environment, but aggregation with HA nanoparticles as a drug carrier showed a steady releasing pattern of HET within 24 hours. The aggregation of biodegraded nanoparticles, such as HA and CHI, has been shown to improve the bioavailability of compounds with poor solubility in water, 17 which is in accordance with the enhancement and improvement of antitumor effects of HET in vitro in the current study.…”

supporting

confidence: 77%

“…16,17 However, the antitumor activities exerted by marine natural compounds encapsulated by these polymeric nanoparticles against urothelial tumor cells have not been studied. In addition, the preapoptotic and antiapoptotic factors involved in the pathways of the biodegradable nanoparticles-aggregated compound-induced apoptosis have not been extensively investigated.…”

Section: Discussionmentioning

confidence: 99%

“…The main conditions of HA/CHI nanoparticle fabrication for aggregation of HET in the EFS are set as follows: temperature (25°C), applied electric field strength (2.5 kV/cm), reaction time (HA: 1 hour, CHI: 5 hours), and cross-linking reagents (HA: 0.001 N FeCl 3 , CHI: 0.001 N NaOH). 16,17 The transmission electron microscopy (TEM) images of HA/CHI nanoparticles, HA/HET, and CHI/HET aggregates are shown in Figure 1.…”

Section: Materials and Methods Materialsmentioning

confidence: 99%

“…17 Briefly, HET was added to a centrifuge tube and then placed on a 40 rpm shaker at 37°C. The sample was centrifuged at 12,000 rpm for 60 minutes before the supernatant was used to determine the amount of HET by high-performance liquid chromatography (Agilent 1100 series; Agilent, Santa Clara, CA, USA).…”

Section: In Vitro Release Studymentioning

confidence: 99%

“…23 We have previously fabricated the biopolymeric nanoparticles using an electrostatic field system (EFS) in an aqueous-phase environment as the drugs/compounds with antitumor activities have been successfully aggregated by the biopolymeric nanoparticles in the EFS. 16,17 In this study, the natural marine compound HET extracted from a sponge Hippospongia sp. was aggregated by biopolymeric HA and CHI nanoparticles using the EFS method.…”

Section: Introductionmentioning

confidence: 99%

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Improvement and enhancement of antibladder carcinoma cell effects of heteronemin by the nanosized hyaluronan aggregation

Huang

Kuo

et al. 2016

IJN

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The effects against tumors exerted by marine active compounds have been highlighted and investigated. Polymeric nanoparticles made from biodegradable and biocompatible molecules such as hyaluronan (HA) and chitosan (CHI) are able to aggregate the compounds to enhance their activities against tumor cells and reduce the toxicity on normal cells. Here, we extensively examined the antitumor activities and the mechanisms of HA/CHI nanoparticles-aggregated heteronemin (HET) extracted from the sponge Hippospongia sp. The half-maximal inhibitory concentration (IC 50 ) of pure HET toward T24 bladder carcinoma cells is ~0.28 µg/mL. Pure HET from 0.2 to 0.8 µg/mL and HA nanoparticles-aggregated HET at 0.1 and 0.2 µg/mL significantly reduced T24 cell viability. Compared to pure HET, HA nanoparticles/HET aggregates showed much weaker viability-inhibitory effects on L929 normal fibroblasts. HET dose-dependently suppressed cancer cell migration as HA/CHI nanoparticles-aggregated HET displayed stronger migration-inhibitory effects than pure HET. Flow cytometric analysis showed that pure HET increased early/total apoptosis and JC-1 monomer fluorescence, while HA/CHI nanoparticles-aggregated HET induced higher apoptosis and JC-1 monomer rates than pure HET, suggesting that aggregation of HA nanoparticles offers HET stronger apoptosis-inducing capacity through mitochondrial depolarization. Western blot analysis showed that HA nanoparticles-aggregated HET further increased mitochondrial-associated, caspase-dependent and caspase-independent, as well as endoplasmic reticulum stress-related factors in comparison with pure HET. These data indicated that pure HET possesses cytotoxic, antimigratory, and apoptosis-inducing effects on bladder cancer cells in vitro, and its induction of apoptosis in bladder carcinoma cells is mainly caspase dependent. Moreover, HA nanoparticle aggregation reinforced the cytotoxic, antimigratory, and apoptosis-inducing activities against bladder carcinoma cells and attenuated the viability-inhibitory effects on normal fibroblasts. This aggregation reinforces antibladder carcinoma effects of HET via diverse routes, including mitochondrial-related/caspase-dependent, caspase-independent, and endoplasmic reticulum stress-related pathways. The current data also strongly suggested that HA/CHI nanoparticles-aggregated HET would be a potential treatment for urothelial cancer in vivo.

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supporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Materials and Methods Materialsmentioning

confidence: 99%

Section: In Vitro Release Studymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Improvement and enhancement of antibladder carcinoma cell effects of heteronemin by the nanosized hyaluronan aggregation

Huang

Kuo

et al. 2016

IJN

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Tailoring of physicochemical properties of nanocarriers for effective anti‐cancer applications

Chakraborty

Dhakshinamurthy

Misra

2017

J Biomedical Materials Res

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Nanotechnology has emerged strongly as a viable option to overcome the challenge of early diagnosis and effective drug delivery, for cancer treatment. Emerging research articles have expounded the advantages of using a specific type of nanomaterial-based system called as "nanocarriers," for anti-cancer therapy. The nanocarrier system is used as a transport unit for targeted drug delivery of the therapeutic drug moiety. In order for the nanocarriers to be effective for anticancer therapy, their physicochemical parameter needs to be tuned so that bio-functionalisation can be achieved to (1) allow drugs being attached to the substrate and for their controlled release, (2) ensure the stability of the nanocarrier up to the point of delivery, and (3) clearance of the nanocarrier after the delivery. It is therefore envisaged that tailoring of the physicochemical properties of nanocarriers can greatly influence their reactivity and interaction in the biological milieu, and this is becoming an important parameter for increasing the efficacy of cancer therapy. This review emphasizes the importance of physicochemical properties of nanocarriers, and how they influence its usage as chemotherapeutic drug carriers. The goal of this review is to present a correlation between the physicochemical properties of the nanocarriers and its intended action, and how their design based on these properties can enhance their cancer combating abilities while minimizing damage to the healthy tissues. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2906-2928, 2017.

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Biopolymer nanoparticles: a strategy to enhance stability, bioavailability, and biological effects of phenolic compounds as functional ingredients

2021

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Phenolic compounds are abundant in nature and have multiple beneficial effects on human health due to their antioxidant, anti-inflammatory, antithrombotic, antiallergenic, anticancer, and antiatherosclerotic properties. For this reason, phenolics are becoming relevant functional ingredients for several industries, mainly the food industry, derived from food consumer exigencies and regulations. However, the use of their beneficial properties still presents some limitations, such as chemical instability under environmental and processing conditions, which leads to structural changes and compromises their biological activities. They also present poor water solubility and sensitivity to pH changes, decreasing their bioavailability in the organism. The technologies for extraction and stabilization of these compounds have evolved rapidly in the development of different delivery systems to encapsulate sensitive active molecules. Biopolymeric nanoparticles are biodegradable polymer-based colloidal systems with sizes ranging from 1 to 1000 nm, and different techniques can be carried out to develop them. These systems have emerged as a green and effective alternative to improve stability, bioavailability, and biological effects of phenolic compounds. This comprehensive review aims to present an overview of recent advances in encapsulation processes of phenolic compounds within biopolymer nanoparticles as delivery systems and the impact on their physicochemical properties and biological effects after encapsulation.

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Enhanced anti-cancer activity by curcumin-loaded hydrogel nanoparticle derived aggregates on A549 lung adenocarcinoma cells

Cited by 35 publications

References 31 publications

Improvement and enhancement of antibladder carcinoma cell effects of heteronemin by the nanosized hyaluronan aggregation

Improvement and enhancement of antibladder carcinoma cell effects of heteronemin by the nanosized hyaluronan aggregation

Tailoring of physicochemical properties of nanocarriers for effective anti‐cancer applications

Biopolymer nanoparticles: a strategy to enhance stability, bioavailability, and biological effects of phenolic compounds as functional ingredients

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