Enhanced antibacterial and antibiofilm functions of the curcumin-mediated photodynamic inactivation against Listeria monocytogenes

Huang, Jiaming; Chen, Bowen; Li, Huihui; Qing-jun, Zeng; Wang, Jing Jing; Liu, Haiquan; Pan, Yingjie; Zhao, Yong

doi:10.1016/j.foodcont.2019.106886

Cited by 85 publications

(53 citation statements)

References 35 publications

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“…These results suggest that resveratrol increased the stress responses of the L. monocytogenes cells to resist the external harsh environment. A similar result was found by Huang et al (2020) , such that the photodynamic inactivation treatment up-regulated the expression of prfA while it markedly reduced the adhesion ability of the biofilms of L. monocytogenes . Therefore, biofilm inhibition is a complex process, suggesting that the biofilm inhibitory pathway of various antimicrobial compounds differs.…”

Section: Discussionsupporting

confidence: 82%

Inhibition of Biofilm Formation and Related Gene Expression of Listeria monocytogenes in Response to Four Natural Antimicrobial Compounds and Sodium Hypochlorite

Liu

Han

et al. 2021

Front. Microbiol.

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The aim of this study was to assess the efficacy of four natural antimicrobial compounds (cinnamaldehyde, eugenol, resveratrol and thymoquinone) plus a control chemical disinfectant (sodium hypochlorite) in inhibiting biofilm formation by Listeria monocytogenes CMCC54004 (Lm 54004) at a minimum inhibitory concentration (MIC) and sub-MICs. Crystal violet staining assay and microscopic examination were employed to investigate anti-biofilm effects of the evaluated compounds, and a real-time PCR assay was used to investigate the expression of critical genes by Lm 54004 biofilm. The results showed that five antimicrobial compounds inhibited Lm 54004 biofilm formation in a dose dependent way. Specifically, cinnamaldehyde and resveratrol showed better anti-biofilm effects at 1/4 × MIC, while sodium hypochlorite exhibited the lowest inhibitory rates. A swimming assay confirmed that natural compounds at sub-MICs suppressed Lm 54004 motility to a low degree. Supporting these findings, expression analysis showed that all four natural compounds at 1/4 × MIC significantly down-regulated quorum sensing genes (agrA, agrC, and agrD) rather than suppressing the motility- and flagella-associated genes (degU, motB, and flaA). This study revealed that sub-MICs of natural antimicrobial compounds reduced biofilm formation by suppressing the quorum sensing system rather than by inhibiting flagella formation.

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Section: Discussionsupporting

confidence: 82%

Inhibition of Biofilm Formation and Related Gene Expression of Listeria monocytogenes in Response to Four Natural Antimicrobial Compounds and Sodium Hypochlorite

Liu

Han

et al. 2021

Front. Microbiol.

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“…Curcumin can be used as a bactericidal agent or photosensitizer to utilize the photodynamic inactivation (PDI) reaction mechanism to achieve a bactericidal effect. Studies have shown that curcumin-mediated PDI can effectively inactivate Listeria monocytogenes cells and eliminate their mature biofilms [ 7 ]. Therefore, the curcumin-mediated PDI treatment can effectively kill some food-borne bacteria, thereby greatly improving the antibacterial effect on food in the process of sterilization and preservation.…”

Section: Introductionmentioning

confidence: 99%

Effects of Emulsifier Type and Post-Treatment on Stability, Curcumin Protection, and Sterilization Ability of Nanoemulsions

Fang

et al. 2021

Foods

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Curcumin has a high inhibitory effect on many potential diseases caused by bacteria and fungi. However, its degradability and low water solubility limit its application. Loading curcumin with an emulsion delivery system can overcome these problems. Five different types of emulsifiers were used to prepare the curcumin-loaded nanoemulsions, namely, Tween 80 (T80), Span 80 (S80), sodium dodecyl sulfate (SDS), soybean protein isolate (SPI), and lecithin (LEC). The effects of emulsifier types and post-treatment methods on emulsion stability and curcumin-load efficiency were studied. In addition, photodynamic inactivation was used to test the antibacterial effect of nanoemulsions on Escherichia coli under blue light excitation. The five types of emulsifiers could form uniform emulsions with good storage stability and with antibacterial capacity on Escherichia coli. Among them, the T80 and LEC emulsions had good stability, coating effect, and sterilization performance under heating or room temperature. Both curcumin-loaded bactericidal emulsions had the potential for large-scale applications. A nanoemulsions delivery system could effectively improve the dispersion and chemical stability of curcumin in water. An emulsion loaded with antibacterial photosensitizer represents a new idea for the storage and preservation of food commodities.

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“…In contrast, L. monocytogenes with MEL-A treatment showed irregular shape with the sunken surfaces (red arrow), and the cross section was much shallower than the control (blue arrow). These results suggested that MEL-A had a stronger impact on the cell membrane and the leakage of intracellular contents including nucleic acids and proteins, which was similar to the effect of carvacrol on L. monocytogenes [ 30 ]. In conclusion, the morphological observation of L. monocytogenes provides compelling evidence that MEL-A is able to damage the cell membrane and eventually result in the leakage of intracellular substances and cell death.…”

Section: Resultsmentioning

confidence: 90%

“…SEM was used to observe the external morphology changes of the L. monocytogenes cells [ 30 ]. The SEM images ( Figure 4 A) of the MEL-A-treated cells were significantly different to those of the untreated cells.…”

Section: Resultsmentioning

confidence: 99%

Antibacterial Efficacy and Mechanism of Mannosylerythritol Lipids-A on Listeria monocytogenes

et al. 2020

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Mannosylerythritol lipids-A (MEL-A) is a novel biosurfactant with excellent surface activity and potential biomedical applications. In this study, we explored the antibacterial activity and the underlying mechanisms of MEL-A against the important food-borne pathogen Listeria monocytogenes. The bacterial growth and survival assays revealed a remarkable antibacterial activity of MEL-A. Since MEL-A is a biosurfactant, we examined the cell membrane integrity and morphological changes of MEL-A-treated bacteria by biochemical assays and flow cytometry analysis and electron microscopes. The results showed obvious damaging effects of MEL-A on the cell membrane and morphology. To further explore the antibacterial mechanism of MEL-A, a transcriptome analysis was performed, which identified 528 differentially expressed genes (DEGs). Gene ontology (GO) analysis revealed that the gene categories of membrane, localization and transport were enriched among the DEGs, and the analysis of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways demonstrated significant changes in the maltodextrin ABC transporter system and stress response system. Furthermore, the growth of L. monocytogenes could also be significantly inhibited by MEL-A in milk, a model of a real food system, suggesting that MEL-A could be potentially applied as an natural antimicrobial agent to control food-borne pathogens in the food industry.

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Enhanced antibacterial and antibiofilm functions of the curcumin-mediated photodynamic inactivation against Listeria monocytogenes

Cited by 85 publications

References 35 publications

Inhibition of Biofilm Formation and Related Gene Expression of Listeria monocytogenes in Response to Four Natural Antimicrobial Compounds and Sodium Hypochlorite

Inhibition of Biofilm Formation and Related Gene Expression of Listeria monocytogenes in Response to Four Natural Antimicrobial Compounds and Sodium Hypochlorite

Effects of Emulsifier Type and Post-Treatment on Stability, Curcumin Protection, and Sterilization Ability of Nanoemulsions

Antibacterial Efficacy and Mechanism of Mannosylerythritol Lipids-A on Listeria monocytogenes

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