Enhanced Antibacterial and Antioxidant Properties of Chitosan Films Blended with Gallic Acid and Incorporated with Thymol Silver Nanoparticles

Abstract

In the present study, an attempt has been made to build and evaluate Chitosan+Glycerol/Gallic acid/Thymol-silver nanoparticles or chitosan blended (C+G/GA/T-SNPs) film to significantly improve antioxidant and antibacterial activity for accelerated wound healing. Methanolic Gallic acid is used for the first time in antibacterial chitosan control (C+G) films. All developed films, compounds was Thymol and Gallic acid and their synthesized Thymol silver nanoparticles (T-SNPs) and Gallic acid silver nanoparticles (GA-SNPs) were characterized by Fourier-transform infrared spectroscopy (FT-IR), Scanning electron microscopy (SEM), X-Ray diffraction (XRD), Zeta potential (ZP), Dynamic light scattering (DLS), and UV-Vis spectroscopy. T-SNPs and GA-SNPs are rod and spherical in shape and were sufficient to reduce, capped, and stabilize. T-SNPs and GA-SNPs were measured Dynamic Light scattering and found to be 123.2 nm and 121.1 nm with surface charges of -19.7 and -20.3 respectively. The incorporation of methanolic Gallic acid and T-SNPs into chitosan films, as predicted, effectively enhanced antioxidant and antimicrobial activity. The antimicrobial activity of Thymol, T-SNPs and C+G/GA/T-SNPs film showed more zone of inhibition than Gallic acid, GA-SNPs and C+G film. The elasticity, texture and folding endurance of the C+G film and C+G/GA/T-SNPs films have been substantially improved. The ecological quality of the generated C+G and C+G/GA/T-SNPs film was determined by the assessment of soil degradation and water degradation parameters. These findings lead to the conclusion that the C+G/GA/T-SNPs film produced with Gallic acid and T-SNPs can improve wound healing.
 Keywords: Chitosan, Gallic Acid, Thymol Silver Nanoparticles, Antibacterial and Antioxidant Properties.

Design of Thymol Loaded Dental In Situ Gels; In Vitro Evaluation

“…To determine the amount of MHL in in situ gel, 1 mL of in situ gel was diluted in 1 mL of ethanol water mixture (Ethanol:Water) 50:50. Thymol concentrations were determined using a UV spectrophotometer (UVmini-1240 Shimadzu) at 278 nm [18,19]. (Calibration of the method was carried out by measuring a series of standards of diluted stock solutions of Thymol.…”