Enhanced anticancer effect of Combretastatin A-4 phosphate when combined with vincristine in the treatment of hepatocellular carcinoma

Aboubakr, Esam M.; Taye, Ashraf; Aly, Omar M.; Gamal‐Eldeen, Amira M.; El‐Moselhy, Mohamed A.

doi:10.1016/j.biopha.2017.02.019

Cited by 20 publications

(16 citation statements)

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“…It has been demonstrated by various investigators that circulating pro-inflammatory cytokines, such as TNF-α and interleukin 6 which triggers hepatic injury, were increased at least in part by a free-radical-mediated apoptotic mechanism. [ 26 ] Augmented production of ROS on DEN induction in GME-treated rats could have been established by the free-radical scavenging potential of alpha-mangostin in preventing the depletion of GSH, thereby contributing to the curtailment in TNF-α expression. Suppressed TNF-α expression by xanthones in the present study is concurrent with the earlier studies.…”

Section: Discussionmentioning

confidence: 99%

Biochemical evidence for the antitumor potential of Garcinia mangostana Linn. On diethylnitrosamine-induced hepatic carcinoma

2018

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Background:Garcinia mangostana is extensively used in most of the Indian herbal pharmaceuticals and nutraceuticals.Objective:The objective of this study was to elucidate the underlying biochemical protective mechanism of G. Mangostana Linn. fruit extract (GME) in deterioration of diethylnitrosamine (DEN)-induced hepatic carcinoma (HCC) in rats.Materials and Methods:The cancer was induced using DEN to the experimental rats and treated with GME (200, 400, and 600 mg/kg) to find its anticancer property. The cancer biomarkers such as alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA), hepatic hydroxyl proline, total tissue protein, and tumor necrosis factor-alpha levels were measured using ELISA. The vascular endothelial growth factor expressions were also seen in liver tissues using immunohistochemistry.Results:In addition, there was a significant increase in serum AFP, CEA, hepatic hydroxylproline, and total tissue protein levels in HCC group versus the negative control group. In contrast, the groups with HCC subjected to either high or low dose of GME elicited significant reduction of AFP, CEA, hepatic hydroxylproline, and increase in total protein in serum compared to the untreated HCC rats. Interestingly, treatment with GME elicited marked improvement in the liver histological feature and downregulation of tumor necrosis factor-alpha levels in HCC groups. GME extract may have chemopreventive benefits by reducing the tumor promoting growth factor levels in HCC-induced group.Conclusion:To sum up, all findings on curative groups had proved clearly that the GME has anticarcinogenic effect on the development of liver cancer induced by DEN in rats.SUMMARY Garcinia mangostana Linn. (GME) may have chemopreventive property by reducing the tumor promoting growth factor and tumor necrosis factor-alpha levels in diethylnitrosamine (DEN)-induced hepatic cancerThe suppression in the levels of cancer biomarkers by GME due to the presence of anticancer phytoconstituentsThe histological studies proved the effective dose of GME against DEN-induced experimental hepatic cancer. Abbreviations used: TNF-α: Tumor necrosis factor-alpha, PBS: Phosphate buffered saline, ROS: Reactive oxygen species, DNA: Deoxyribonucleic acid, GSH: Glutathione, VEGF: Vascular endothelial growth factor.

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Section: Discussionmentioning

confidence: 99%

Biochemical evidence for the antitumor potential of Garcinia mangostana Linn. On diethylnitrosamine-induced hepatic carcinoma

2018

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“…Imaging studies of HeLa cells treated with FITC-loaded micelles showed a rapid internalization. Based on these results, in vivo studies to determine the anticancer activity were suggested [84].…”

Section: Polymeric-based Nanoformulationsmentioning

confidence: 99%

Recent Advances and Impact of Chemotherapeutic and Antiangiogenic Nanoformulations for Combination Cancer Therapy

Rajora

Ravishankar²,

Zhang

et al. 2020

Pharmaceutics

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Traditional chemotherapy, along with antiangiogenesis drugs (combination cancer therapy), has shown reduced tumor recurrence and improved antitumor effects, as tumor growth and metastasis are often dependent on tumor vascularization. However, the effect of combination chemotherapy, including synergism and additive and even antagonism effects, depends on drug combinations in an optimized ratio. Hence, nanoformulations are ideal, demonstrating a great potential for the combination therapy of chemo-antiangiogenesis for cancer. The rationale for designing various nanocarriers for combination therapy is derived from organic (polymer, lipid), inorganic, or hybrid materials. In particular, hybrid nanocarriers that consist of more than one material construct provide flexibility for different modes of entrapment within the same carrier—e.g., physical adsorption, encapsulation, and chemical conjugation strategies. These multifunctional nanocarriers can thus be used to co-deliver chemo- and antiangiogenesis drugs with tunable drug release at target sites. Hence, this review attempts to survey the most recent advances in nanoformulations and their impact on cancer treatment in a combined regimen—i.e., conventional cytotoxic and antiangiogenesis agents. The mechanisms and site-specific co-delivery strategies are also discussed herein, along with future prospects.

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“…NAD (P)H‐dependent oxidoreductase or dehydrogenases in viable cells can reduce MTT into purple‐colored formazan, which can then be solubilized for spectrophotometric analysis (Berridge & Tan, ; Gerlier & Thomasset, ). A study carried out by Aboubakr, Taye, Aly, Gamal‐Eldeen, and El‐Moselhy () used the MTT assay to evaluate effects of combinations of combretastatin A‐4 and vincristine in hepatocellular carcinoma (Hep G2) cell viability. Another recent study carried out by Pérez‐Salvia et al () demonstrated antiproliferative effects of BET bromodomain inhibitor JQ1 in MCF‐7 and T47D breast cancer cell lines using the MTT assay.…”

Section: Cell Viability/cytotoxicity and Antiproliferative Assaysmentioning

confidence: 99%

“…NAD (P)H-dependent oxidoreductase or dehydrogenases in viable cells can reduce MTT into purple-colored formazan, which can then be solubilized for spectrophotometric analysis (Berridge & Tan, 1993;Gerlier & Thomasset, 1986). A study carried out by Aboubakr, Taye, Aly, Gamal-Eldeen, and El-Moselhy (2017) 2H-tetrazolium), XTT (2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2Htetrazolium-5-carboxanilide) and WST (reagent series) can also generate formazan upon reduction by oxidoreductase or dehydrogenase (Riss et al, 2004) (Figure 2). Unlike the normal MTT assay, assays with these regents (MTS, XTT and WST) do not require formazan solubilization step as solubilization occurs in the cell medium itself (Riss et al, 2004;Stoddart, 2011).…”

Section: Introductionmentioning

confidence: 99%

In vitro assays and techniques utilized in anticancer drug discovery

Ediriweera

Tennekoon

Samarakoon

2018

J of Applied Toxicology

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Development of a cancer is a multistep process and six major hallmarks of cancer that are known to control malignant transformation have been described. Anticancer drug development is a tedious process, requiring a number of in vitro, in vivo and clinical studies. In vitro assays provide an initial platform for cancer drug discovery approaches. A wide range of in vitro assays/techniques have been developed to evaluate each hallmark feature of cancer and selection of a particular in vitro assay or technique mainly depends on the specific research question (s) to be examined. In the present review, we have described some commonly utilized in vitro assays and techniques used to examine cell viability/proliferation, apoptosis, cellular senescence, invasion and migration, oxidative stress and antioxidant effects, gene and protein expression, angiogenesis and genomic alterations in cancer drug discovery. Additionally, uses of modern techniques such as high throughput screening, high content screening and reporter gene assays in cancer drug discovery have also been described.

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Enhanced anticancer effect of Combretastatin A-4 phosphate when combined with vincristine in the treatment of hepatocellular carcinoma

Cited by 20 publications

References 54 publications

Biochemical evidence for the antitumor potential of Garcinia mangostana Linn. On diethylnitrosamine-induced hepatic carcinoma

Biochemical evidence for the antitumor potential of Garcinia mangostana Linn. On diethylnitrosamine-induced hepatic carcinoma

Recent Advances and Impact of Chemotherapeutic and Antiangiogenic Nanoformulations for Combination Cancer Therapy

In vitro assays and techniques utilized in anticancer drug discovery

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