Enhanced antifungal effects of amphotericin B-TPGS-b-(PCL-ran-PGA) nanoparticles in vitro and in vivo

Tang, Xiaolong; Zhu, He; Sun, Lichang; Hou, Wei; Cai, Shuyu; Zhang, Rongbo; Liu, Feng

doi:10.2147/ijn.s71623

Cited by 20 publications

(16 citation statements)

References 26 publications

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“…AmB has been used for decades to treat common invasive fungal infections. Although AmB has high nephrotoxicity, nanoparticle formulations of AmB have been shown to have reduced toxicity compared to its monomer state (21,22). Similarly, the results of the present study revealed lower toxicity of AmB-NPs than free AmB, including the hemolytic activity of red blood cells and the activity of macrophages (Fig.…”

Section: Discussionsupporting

confidence: 80%

Synergistic Antifungal Effect of Amphotericin B-Loaded Poly(Lactic-Co-Glycolic Acid) Nanoparticles and Ultrasound against Candida albicans Biofilms

Yang¹,

Hou³

et al. 2019

Antimicrob Agents Chemother

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Candida albicansis a human opportunistic pathogen that causes superficial and life-threatening infections. An important reason for the failure of current antifungal drugs is related to biofilm formation, mostly associated with implanted medical devices. The present study investigated the synergistic antifungal efficacy of low-frequency and low-intensity ultrasound combined with amphotericin B (AmB)-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles (AmB-NPs) againstC. albicansbiofilms. AmB-NPs were prepared by a double-emulsion method and demonstrated lower toxicity than free AmB. We then established biofilms and treated them with ultrasound and AmB-NPs separately or jointlyin vitroandin vivo. The results demonstrated that the activity, biomass, and proteinase and phospholipase activities of biofilms were decreased significantly after the combination treatment of AmB-NPs with 42 kHz of ultrasound irradiation at an intensity of 0.30 W/cm2for 15 min compared with the controls, with AmB alone, or with ultrasound treatment alone (P < 0.01). The morphology of the biofilms was altered remarkably after joint treatment based on confocal laser scanning microscopy (CLSM), especially in regard to reduced thickness and loosened structure. Furthermore, the same synergistic effects were found in a subcutaneous catheter biofilm rat model. The number of CFU from the catheter exhibited a significant reduction after joint treatment with AmB-NP and ultrasound for seven continuous days, and CLSM and scanning electron microscopy (SEM) images revealed that the biofilm on the catheter surface was substantially eliminated. This method may provide a new noninvasive, safe, and effective therapy forC. albicansbiofilm infection.

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Section: Discussionsupporting

confidence: 80%

Synergistic Antifungal Effect of Amphotericin B-Loaded Poly(Lactic-Co-Glycolic Acid) Nanoparticles and Ultrasound against Candida albicans Biofilms

Yang¹,

Hou³

et al. 2019

Antimicrob Agents Chemother

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“…Using nano technology could possibly change the properties of the substances. Zeta potential is an important criterion for the stability of a colloidal system, and a minimum ZP of ± 20 reported by different studies is desirable (16,17). Cholesterol was used to improve stability because cholesterol can increase zeta potential (18).…”

Section: Discussionmentioning

confidence: 99%

In vitro Evaluation of the Effects of Fluconazole and Nano-Fluconazole on A. flavus and Aspergillus fumigatus Isolates

Sarrafha

Hashemi

Rezaei

et al. 2018

Jundishapur J Microbiol

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Background: Increasing azole resistance among Aspergillus strains was observed over the last decade. For this reason, viable alternatives for the current medicines are required. Objectives: The current study aimed at producing fluconazole-loaded liposomal nanoparticles and comparing in vitro antifungal activity of fluconazole and nano-fluconazole on Aspergillus flavus and A. fumigatus species isolated from patients and poultry. Methods: Fifty isolates of A. flavus and A. fumigatus were collected from visceral and superficial fungal lesions of humans as well as pulmonary fungal infection of poultry. Liposomal nanoparticles were prepared by thin-film hydration method, using soybean lecithin, cholesterol, and fluconazole in a ratio of 10:1:1. The nanoparticles were analyzed in terms of size, zeta potential, and morphology. Antifungal susceptibility testing was carried out to examine and evaluate the minimum inhibitory concentration (MIC) of fluconazole and nano-fluconazole against Aspergillus species by the standard method of broth microdilution as described in CLSI (the clinical and laboratory standards institute) -M38A2.

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“…The in vivo therapeutic efficacy of the prepared nanoparticles was tested by a described method [21]. Sixty 20–22-g BALB/c mice (6–8 weeks old) were infected by inhalation with a volume of 20 μL of PBS containing 5.0 × 10 5 contagious C. albicans cells.…”

Section: Methodsmentioning

confidence: 99%

Enhanced Antifungal Activity by Ab-Modified Amphotericin B-Loaded Nanoparticles Using a pH-Responsive Block Copolymer

et al. 2015

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Fungal infections are an important cause of morbidity and mortality in immunocompromised patients. Amphotericin B (AMB), with broad-spectrum antifungal activity, has long been recognized as a powerful fungicidal drug, but its clinical toxicities mainly nephrotoxicity and poor solubility limit its wide application in clinical practice. The fungal metabolism along with the host immune response usually generates acidity at sites of infection, resulting in loss of AMB activity in a pH-dependent manner. Herein, we developed pH-responsive AMB-loaded and surface charge-switching poly(d,l-lactic-co-glycolic acid)-b-poly(l-histidine)-b-poly(ethylene glycol) (PLGA-PLH-PEG) nanoparticles for resolving the localized acidity problem and enhance the antifungal efficacy of AMB. Moreover, we modified AMB-encapsulated PLGA-PLH-PEG nanoparticles with anti-Candida albicans antibody (CDA) (CDA-AMB-NPs) to increase the targetability. Then, CDA-AMB-NPs were characterized in terms of physical characteristics, in vitro drug release, stability, drug encapsulation efficiency, and toxicity. Finally, the targetability and antifungal activity of CDA-AMB-NPs were investigated in vitro/in vivo. The result demonstrated that CDA-AMB-NPs significantly improve the targetability and bioavailability of AMB and thus improve its antifungal activity and reduce its toxicity. These NPs may become a good drug carrier for antifungal treatment.

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Enhanced antifungal effects of amphotericin B-TPGS-b-(PCL-ran-PGA) nanoparticles in vitro and in vivo

Cited by 20 publications

References 26 publications

Synergistic Antifungal Effect of Amphotericin B-Loaded Poly(Lactic-Co-Glycolic Acid) Nanoparticles and Ultrasound against Candida albicans Biofilms

Synergistic Antifungal Effect of Amphotericin B-Loaded Poly(Lactic-Co-Glycolic Acid) Nanoparticles and Ultrasound against Candida albicans Biofilms

In vitro Evaluation of the Effects of Fluconazole and Nano-Fluconazole on A. flavus and Aspergillus fumigatus Isolates

Enhanced Antifungal Activity by Ab-Modified Amphotericin B-Loaded Nanoparticles Using a pH-Responsive Block Copolymer

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