Enhanced antimicrobial, antioxidant and anticancer activity of Rhizophora apiculata: An experimental report

Abstract

The present study is designed to evaluate the antimicrobial, antioxidant and anticancer activities of . Initially, the phenolic and flavonoid content was quantified in solvent extracts, and gallic acid and rutin were used as a control, respectively. Further, antimicrobial and minimal inhibitory activities of different solvent extracts were assessed against human clinical pathogenic bacteria, and the results showed that butanol and methanol extract has potential antimicrobial activity. FTIR analysis of solvent extracts showed the presence of phenolic compounds at 3409-3430 cm that actively involved in various applications including antioxidant and anticancer activities. The in vitro antioxidant activity of solvent extracts showed excellent antioxidant potential, about 84% of DPPH free-radical scavenging, 76% of hydrogen peroxide, 82% of hydroxyl radical scavenging, and 80% of reducing power. Two-way ANOVA analysis showed that the highly significant effect of antioxidant activity depends on the concentration of extracts. The DNA protection efficiency of extracts against oxidative damage was confirmed by DNA nicking assay using bacterial DNA. The methanol extract effectively inhibited the growth and induces the apoptosis through ROS generation and sensitizes the mitochondrial membrane potential of A549 lung cancer cells. Taken together, the results showed that the solvent extracts of could be potential antioxidant and anticancer agents.

In Vitro Antioxidant Activities of Phenols and Oleanolic Acid from Mango Peel and Their Cytotoxic Effect on A549 Cell Line

“…The experimental group, positive control group and blank group of medium, with sterile PBS washing, with trypsin digestion cells, using RPMI 1640 culture medium containing 10% fetal bovine serum termination of digestion, made the cell suspension, the cell suspension put in centrifuge tube, 1000 r/min, the centrifugal 5 min, abandon supernatant. Plus 5 μL DAPI (0.2 mg·mL −1 ) (Sigma-Aldrich) and kept in dark at 37 °C for 30 min, after the incubation, washing with buffer, add 1 mL culture, in the inverted fluorescence microscope observed under fluorescent green fluorescence set (Olympus Co., Tokyo, Japan) and take photos, and use the Image-ProPlus 5.1 software (Media Cybernetics, Rockville, MD, USA) processing images, green fluorescence intensity ratio calculation [ 19 , 20 ].…”

Phenolic Constituents From the Stem Barks of Rhizophora Apiculata Blume

“…Based on the spectroscopic analysis and comparison with literature values, the remaining compounds were identified as 2,6-dimethoxy- [1,4]benzoquinone (3) [28], 2,4,6trimethoxyphenol (4) [29], 3,4,5-trimethoxybenzyl alcohol (5) [30], hydroxytyrosol (6) [31], and methyl 3,4-dihydroxycinnamate (7) [32].…”

“…have resulted in the isolation of various classes of compounds including aliphatic alcohols [10], alkaloids [6,11], flavonoids [6], phenolic derivatives [6], and terpenoids [5,12,13]. Some of these compounds have shown interesting biological properties including antibiotics [11], anticancer [4], antimicrobial [4], antioxidant [4,[14][15][16][17], antiviral [18], and hepatoprotective effects [19].…”

Marine plant mediated green synthesis of silver nanoparticles using mangrove Rhizophora stylosa: Effect of variable process and their antibacterial activity

“…Some studies report that Rhizophora has several biological activities such as antibacterial, antioxidant and anti-cancer. [6][7][8][9] Teraxerol, teraxerone (triterpenoids), sitosterol (steroids), protocatechuic acid and isovanillic acid (phenolic compounds), routine, astilbin, catechin (flavonoids) are the main compounds in RS mangrove plants. Those compounds show extraordinary pharmacological activities and are linked with the presence of -OH.…”