Enhanced antineoplastic/therapeutic efficacy using 5-fluorouracil-loaded calcium phosphate nanoparticles

Mohiyuddin, Shanid; Naqvi, Saba; Gopinath, P.

doi:10.3762/bjnano.9.233

Cited by 17 publications

(6 citation statements)

References 53 publications

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“…Future work should focus on other ways to control the release of organic substances from these nanoparticles and the possibility of extending their release. In terms of size and trapping efficiency, and release of an external agent, these results were comparable to the findings of similar work, but considerably more effective than those synthesized by other groups [36][37][38][39][40][41][42].…”

Section: Discussionsupporting

confidence: 87%

Entrapment of organic fluorophores in calcium phosphate nanoparticles with slow release

Sadallah¹,

Boukhris²,

Hannache³

et al. 2020

Turk J Chem

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Two organic fluorophores, fluorescein (F) and rhodamine B (Rd), were entrapped in calcium phosphate nanoparticles. The as-obtained nanoparticles can be used for biological release applications. For this aim, calcium phosphate nanoparticles were synthesized using the precipitation method. Structural analysis of these nanoparticles was performed using XRD, FTIR, and Raman spectroscopy, confirming that the synthesized nanoparticles were hydroxyapatite. TEM and SEM analyses demonstrated that these nanoparticles had a size of 20 nm and a well-defined morphology. F and Rd (about 0.5 wt.%) were entrapped in these nanoparticles and their release, as a function of time, was studied via UV-Vis spectroscopy. The obtained results showed that the release of both fluorophores was progressive over time. The trapping efficiencies of the fluorophores were 67.15% and 90.76% for F and Rd, respectively.

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Section: Discussionsupporting

confidence: 87%

Entrapment of organic fluorophores in calcium phosphate nanoparticles with slow release

Sadallah¹,

Boukhris²,

Hannache³

et al. 2020

Turk J Chem

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“…Through this mechanism of action, ROCK1 eventually leads to membrane blebbing and cell contraction [23,24]. Similar morphological changes were observed in breast cancer due to gef gene expression [25] and in lung and colon cancer cells after 5-fluorouracil-loaded calcium phosphate nanoparticle treatment [26]. This data is in agreement with our previous publication in which we analyzed, using flow cytometry with annexin V and propidium iodide and transmission electron microscopy, the effect of gef and apoptin genes on the DLD-1 cell line.…”

Section: Discussionmentioning

confidence: 86%

Deciphering the Mechanism of Action Involved in Enhanced Suicide Gene Colon Cancer Cell Killer Effect Mediated by Gef and Apoptin

Cáceres¹,

Ramírez

Carrillo

et al. 2019

Cancers

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Despite the great advances in cancer treatment, colorectal cancer has emerged as the second highest cause of death from cancer worldwide. For this type of tumor, the use of suicide gene therapy could represent a novel therapy. We recently demonstrated that co-expression of gef and apoptin dramatically inhibits proliferation of the DLD-1 colon cell line. In the present manuscript, we try to establish the mechanism underlying the enhanced induction of apoptosis by triggering both gef and apoptin expression in colon tumor cells. Scanning microscopy reveals that simultaneous expression of gef and apoptin induces the apparition of many “pores” in the cytoplasmic membrane not detected in control cell lines. The formation of pores induced by the gef gene and accentuated by apoptin results in cell death by necrosis. Moreover, we observed the presence of apoptotic cells. Performing protein expression analysis using western blot, we revealed an activation of mitochondrial apoptosis (increased expression of Pp53, cytochrome c, Bax, and caspase 9) and also the involvement of the extrinsic pathway through caspase 8activation. In conclusion, in this manuscript we demonstrate for the first time that the extrinsic pathway of apoptosis and pore formation is also involved in the cell death caused by the co-expression of the gef and apoptin genes. Our results suggest that co-expression of gef and apoptin genes induces an increase in post-apoptotic necrotic cell death and could be a valuable tool in the design of new antitumor strategies focused on the enhancement of the immune response against cancer cell death.

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“…After treatment with 5-fluorouracilloaded calcium phosphate nanoparticles, Mohiyudin and colleagues discovered that, in comparison to the control group, Bcl-2 was down-regulated, and caspase 3 was up-regulated. [55] Bcl-2 is a member of protein families that play a vital function in the control of apoptosis in cells. [56] The protooncogene Bcl-2 inhibits cell apoptosis when it is upregulated.…”

Section: Discussionmentioning

confidence: 99%

“…According to the findings, 5‐FU‐GA‐CS‐CQD‐Apt may induce apoptosis by means of the mitochondrial pathway. After treatment with 5‐fluorouracil‐loaded calcium phosphate nanoparticles, Mohiyudin and colleagues discovered that, in comparison to the control group, Bcl‐2 was down‐regulated, and caspase 3 was up‐regulated [55] …”

Section: Discussionmentioning

confidence: 99%

Targeted Anticancer Drug Delivery Using Chitosan, Carbon Quantum Dots, and Aptamers to Deliver Ganoderic Acid and 5‐Fluorouracil

Mazandarani,

Taravati,

Mohammadnejad

et al. 2023

Chemistry & Biodiversity

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Breast cancer is a deadly disease that usually affects women. The nano‐drug delivery system's ligand‐functionalized nanoparticles have great cancer‐treatment potential. This study developed a potential method for drug loading and sustained release of breast cancer model medications 5‐fluorouracil (5‐FU) and Ganoderic acid (GA). CS/Apt/COQ NPs were produced. FT‐IR, XRD, DLS, zeta potentiometer, and SEM analyzed NP's chemical structure, particle size, and shape. In vitro MTT experiments examined MCF‐7 cell viability. Real‐time PCR quantified cell apoptosis. XRD and FTIR showed nanocarrier generation, crystalline structure, and molecular interactions. DLS indicated that nanocarriers contain 250.6 nm NPs with PDI 0.057. Zeta potential and SEM indicated their spherical shape and +37.8 mV surface charge. pH 5.4 released most 5‐FU and GA in 48 h. 5‐FU entrapment efficiency was 84.7 ± 5.2 and GA 80.2% ± 2.3. The 5‐FU‐GA‐CS‐CQD‐Apt group killed the most MCF‐7 cells, 57.9%. 5‐FU and GA in CS‐CQD‐Apt increased flow cytometry‐induced apoptosis. 5‐FU‐GA‐CS‐CQD‐Apt increased Caspase 9 and decreased Bcl2. Thus, pH‐sensitive NPs may release 5‐FU and GA to treat breast cancer.

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Enhanced antineoplastic/therapeutic efficacy using 5-fluorouracil-loaded calcium phosphate nanoparticles

Cited by 17 publications

References 53 publications

Entrapment of organic fluorophores in calcium phosphate nanoparticles with slow release

Entrapment of organic fluorophores in calcium phosphate nanoparticles with slow release

Deciphering the Mechanism of Action Involved in Enhanced Suicide Gene Colon Cancer Cell Killer Effect Mediated by Gef and Apoptin

Targeted Anticancer Drug Delivery Using Chitosan, Carbon Quantum Dots, and Aptamers to Deliver Ganoderic Acid and 5‐Fluorouracil

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