Enhanced aptasensor performance for targeted HER2 breast cancer detection by using screen-printed electrodes modified with Au nanoparticles

Harahsheh, Tasneem; Makableh, Yahia F.; Rawashdeh, Isra; Al-Fandi, Mohamed

doi:10.1007/s10544-021-00586-9

Cited by 21 publications

(8 citation statements)

References 45 publications

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“…This was owing to the construction of a thiolated aptamer SAM on the WE surface, and a similar observation was made in different literatures [ 64 , 65 ]. The roughness after the formation of SAM was decreased by immobilization of the target PSA due to smooth coverage of the topography [ 48 , 66 ]. The obtained results regarding the surface roughness can be well correlated with charge transfer resistance and double layer capacitance obtained from EIS.…”

Section: Resultsmentioning

confidence: 99%

Gold Nanoparticles-MWCNT Based Aptasensor for Early Diagnosis of Prostate Cancer

et al. 2022

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Prostate cancer is one of the most frequently diagnosed male malignancies and can be detected by prostate-specific antigen (PSA) as a biomarker. To detect PSA, several studies have proposed using antibodies, which are not economical and require a long reaction time. In this study, we propose to use self-assembled thiolated single-strand DNA on electrodes functionalized by multi-walled carbon nanotubes (MWCNT) modified with gold nanoparticles (AuNPs) to realize a low-cost label-free electrochemical biosensor. In this regard, the PSA aptamer was immobilized via electrostatic adsorption on the surface of a screen-printed MWCNT/AuNPs electrode. The immobilization process was enhanced due to the presence of Au nanoparticles on the surface of the electrode. Surface characterization of the electrode at different stages of modification was performed by electrochemical impedance spectroscopy (EIS), atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR) and contact angle for surface tension properties. The results showed an increase in surface roughness due to the absorbance of the aptamer on the electrode surfaces. The developed sensor has an extended linear range of 1–100 ng/mL, and a very low limit of detection down to 1 pg/mL. In addition, the reaction has a binding time of only five minutes on the developed electrodes. Investigations of the biosensor selectivity against several substances revealed an efficient selectivity for PSA detection. With this approach, low-cost biosensors with high sensitivity can be realized which have a wide linearity range and a low limit of detection, which are necessary for the early detection of prostate cancer.

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Section: Resultsmentioning

confidence: 99%

Gold Nanoparticles-MWCNT Based Aptasensor for Early Diagnosis of Prostate Cancer

et al. 2022

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“…IBC is a common type with a high degree of malignancy, threatening women's life and health [ 17 ]. At present, there are many studies on breast cancer, but its specific etiology is still unknown.…”

Section: Discussionmentioning

confidence: 99%

[Retracted] Correlation Analysis of Ultrasound Elastography Score with Invasive Breast Cancer and Biological Prognostic Factors

Wan

Qin

et al. 2022

Contrast Media & Molecular Imaging

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Invasive breast cancer (IBC) is a kind of malignant tumor in which cancer cells have broken through the basement membrane of breast ducts or lobular acini and invaded the stroma. Although ultrasound elastography score (UES) has shown unique advantages in the diagnosis of IBC, its value in the prognosis is not clear. Here, we explored the correlation of UES with IBC and biological prognostic factors. The datum of 86 patients with suspected IBC from January 2018 to December 2021 was collected. UE was applied in the examination of all patients. The lesion tissue of the malignant group was punctured to detect and analyze the expression of biological prognostic factors, including estrogen receptor (E receptor), progesterone receptor (P receptor), and human epidermal growth factor 2 (HER factor 2) and Ki67. The differences in UES under different biological prognostic factors were compared. The receiver operating characteristic (ROC) curve was applied to analyze the diagnostic value of UES of IBC and the expression of biological prognostic factors. Based on the pathological diagnosis results, the sensitivity, specificity, accuracy, positive predictive value, and negative predictive value of UES in the diagnosis of IBC were analyzed. The correlation of UES with IBC and biological prognostic factors was analyzed by multiple linear regression and Spearman method. ROC analysis showed that the area under the curve of UES for diagnosing IBC and evaluating the expression of P receptor, HER factor 2, and Ki67 were 0.877, 0.704, 0.763, and 0.820, respectively (P＜0.05). The sensitivity, specificity, accuracy, positive predictive value, and negative predictive value of UES when diagnosing IBC were 92.42%, 90.00%, 91.86%, 96.83%, and 78.26%, respectively. The UES of E receptor expression (positive and negative group) showed no obvious variance ( P > 0.05). The UES of P receptors (positive and negative), HER factor 2 (positive and negative), and Ki67 (high and low expression) showed obvious differences ( P < 0.05). Multiple linear regression and Spearman indicated UES was significantly correlated with the expression of P receptor, HER factor 2, and Ki67 (P＜0.05). UES has a certain diagnostic value for IBC and is significantly correlated to the expression of P receptor, HER factor 2, and Ki67, which is helpful for evaluating the prognosis of patients with IBC.

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“…As such, using EIS the sensor achieved a wide linear range of 1 pg mL −1 -100 ng mL −1 , a LOD of 172 pg mL −1 and appropriate detection in undiluted human serum. Instead of utilising an full Au-SPE, it is common practice to utilise Au nanoparticles (NPs) on a standard C-SPE, 116 providing the beneficial properties of nanoparticles and the potential for bioconjugation using simple thiols. 117 Marques and co-workers utilised the AuNPs on a C-SPE surface in conjunction with a sandwich style assay.…”

Section: Sensors and Diagnostics Perspectivementioning

confidence: 99%

Electroanalytical overview: screen-printed electrochemical sensing platforms for the detection of vital cardiac, cancer and inflammatory biomarkers

et al. 2022

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Enhanced aptasensor performance for targeted HER2 breast cancer detection by using screen-printed electrodes modified with Au nanoparticles

Cited by 21 publications

References 45 publications

Gold Nanoparticles-MWCNT Based Aptasensor for Early Diagnosis of Prostate Cancer

Gold Nanoparticles-MWCNT Based Aptasensor for Early Diagnosis of Prostate Cancer

[Retracted] Correlation Analysis of Ultrasound Elastography Score with Invasive Breast Cancer and Biological Prognostic Factors

Electroanalytical overview: screen-printed electrochemical sensing platforms for the detection of vital cardiac, cancer and inflammatory biomarkers

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