Enhanced Bacterial α-Amylase Production Using Mutant Strains Through Submerged Fermentation

Farooq, Muhammad; Ali, Shaukat; Hassan, Ali; Sulayman, Rida; Kaleem, Muhammad Ahsan; Shahzad, Hafsa; Summer, Muhammad; Latif, A.; Tanveer, Tahreem

doi:10.17582/journal.pujz/38.1.99.107

PUJZ

2023

DOI: 10.17582/journal.pujz/38.1.99.107

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Enhanced Bacterial α-Amylase Production Using Mutant Strains Through Submerged Fermentation

Muhammad Farooq¹,

Shaukat Ali²,

Ali Hassan³

et al.

Abstract: they are simple and inexpensive to produce (Gopinath et al., 2017). Amylases are well-known enzymes due to their large number of applications in food, paper, textile, distilling, brewing and pharmaceutical industries (Chimata et al., 2010). Amylases account for approximately 25% of the global enzyme market (Abedi et al., 2022).

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2024

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Economic production of eco-friendly dehairing keratinase and antioxidant feather hydrolyzate using Bacillus halotolerans

Ismail,

Nour,

Nashy

et al. 2024

Biomass Conv. Bioref.

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Feather is a high recalcitrance keratin-based biomass, and keratin-degrading microorganisms have been seen as beneficial tools for its biodegradation and production of valuable products with biomedical and industrial applications. In the current study, the isolated keratinolytic bacteria was identified on the base of its 16 S rDNA sequencing data as Bacillus halotolerans, and its efficiency in the hydrolysis of feather with the preservation of most of its amino acid constituent was estimated by high-performance liquid chromatography Pico-Tag (HPLC PICO-TAG) method. The produced hydrolysate possessed antioxidant activity of 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity of 52.3 ± 5.6 mmole TE/g without possessing any cytotoxicity to human normal skin fibroplast cell line BJ-1 estimated by MTT assay. Moreover, the isolated strain was capable for producing keratinase under submerged fermentation of feather that was optimized by combining single-variable-at-a-time optimization with the application of statistical designs (Plackett-Burman and Box-Behnken). The optimum keratinase activity was 140.83 U/mL that was enhanced by 3.2-fold of the estimated initial activity, and the purified enzyme possessed optimum activity at pH 9.5 and 70 °C with a half-life of 69.3 min. Finally, the applicability of the purified enzyme in the dehairing of bovine hide was examined in comparison to sodium sulfide (Na2S) and lime (Ca(OH)2) conventional method. The results estimated the efficiency of the examined bio-treatment process, it achieved complete dehairing after 2 h with a good skin quality as manifested by scanning electron microscope (SEM). In addition, a significant reduction of the pollution load parameters was estimated.

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Economic production of eco-friendly dehairing keratinase and antioxidant feather hydrolyzate using Bacillus halotolerans

Ismail,

Nour,

Nashy

et al. 2024

Biomass Conv. Bioref.

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show abstract

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Enhanced Bacterial α-Amylase Production Using Mutant Strains Through Submerged Fermentation

Cited by 1 publication

References 36 publications

Economic production of eco-friendly dehairing keratinase and antioxidant feather hydrolyzate using Bacillus halotolerans

Economic production of eco-friendly dehairing keratinase and antioxidant feather hydrolyzate using Bacillus halotolerans

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