Enhanced Bioactivity of Enzyme/MOF Biocomposite via Host Framework Engineering

Abstract

This study reports the successful development of a sustainable synthesis protocol for a phase-pure metal azolate framework (MAF-6) and its application in enzyme immobilization. An esterase@MAF-6 biocomposite was synthesized, and its catalytic performance was compared with that of esterase@ZIF-8 and esterase@ZIF-90 in transesterification reactions. Esterase@MAF-6, with its large pore aperture, showed superior enzymatic performance compared to esterase@ZIF-8 and esterase@ZIF-90 in catalyzing transesterification reactions using both n-propanol and benzyl alcohol as reactants. The hydrophobic nature of the MAF-6 platform was shown to activate the immobilized esterase into its open-lid conformation, which exhibited a 1.5- and 4-times enzymatic activity as compared to free esterase in catalyzing transesterification reaction using n-propanol and benzyl alcohol, respectively. The present work offers insights into the potential of MAF-6 as a promising matrix for enzyme immobilization and highlights the need to explore MOF matrices with expanded pore apertures to broaden their practical applications in biocatalysis.

Highly Enantioselective Catalysis by Enzyme Encapsulated in Metal Azolate Frameworks with Micelle-Controlled Pore Sizes

“…Traditional MAF-6 synthesis, common in typical ZIF synthesis, requires bases, ethanol solvent, organic templates, and high temperature risking enzyme deactivation. To meet our goal of an advanced biocatalyst, it is crucial to develop a synthetic approach for MAF-6 that prioritizes enzyme compatibility and environmental sustainability …”

“…To meet our goal of an advanced biocatalyst, it is crucial to develop a synthetic approach for MAF-6 that prioritizes enzyme compatibility and environmental sustainability. 41 In our study, we used water-soluble Zn(NO 3 ) 2 as the Zn 2+ source. Maintaining a constant Zn 2+ concentration (0.02 mol L −1 , 1 equiv) in aqueous solution at room temperature, we varied the 2-ethylimidazole (HeIM) concentration from 5 to 80 equiv relative to Zn 2+ .…”

Facile Construction of Stable Hierarchical Porous Metal–Organic Frameworks for In Situ Immobilization of β-Glucosidase Based on Competitive Coordination and Selective Etching Strategies

“…This method is economical, green, easy to process, and has minimal effect on the conformation of the enzyme. For large enzymes, it is especially advantageous. − In spite of many encouraging research reports, − most of the MOFs behave mainly as microporous or small mesoporous materials due to the limited reaction condition synthesis. Hierarchical porous enzyme@MOF composites reported in the literature were usually prepared by a hydrothermal method.…”

Recent Progress on Conversion of Lignocellulosic Biomass by MOF-Immobilized Enzyme

“…In light of the active sites and ligand functionalization, MOFs and their derivatives could be used to catalyze lignocellulosic biomass to various products through hydrolysis, dehydration, isomerization, oxidation, etc. Recently, MOFs attracted considerable attention as potential materials for enzyme immobilization [19][20][21][22]. Various organic linkers and metal nodes are available to tailor MOFs with tunable properties so that MOFs can be adapted for the fixing and protection of enzymes [23].…”