Abstract:Nanofibers have attracted increasing attention in drug delivery and other biomedical applications due to their some special properties. The present study aims to prepare a fiber-based nanosolid dispersion system to enhance the bioavailability of curcumin (CUR). CUR-loaded polyvinyl pyrrolidone (CUR@PVP) nanofibers were successfully prepared via electrospinning. Scanning electron microscopy (SEM) was employed to observe the morphology of the nanofibers, and the SEM image showed that the drug-loaded nanofibers w… Show more
“…For instance, the average diameter of PVP fibers was decreased following the incorporation of curcumin. 24 The same …”
Section: Morphology Of the Filamentsmentioning
confidence: 68%
“…[15][16][17][18] Numerous studies have proposed electrospun meshes loaded with curcumin, given the potential for health care applications. A wide range of polymers has been used, including polycaprolactone (PCL), [19][20][21] polylactic acid (PLA), 22 polylactic-co-glycolic acid, 23 polyvinylpyrrolidone (PVP), 24 silk, 25 and cellulose acetate. 26 Because of the hydrophobic nature of curcumin, the use of hydrophobic polymers such as degradable polyesters is particularly attractive to achieve a slow and prolonged release.…”
Section: Introductionmentioning
confidence: 99%
“…This result, together with the mixed results obtained from the scratch test, indicated that C1 might be at the threshold of being toxic for NHDFs. Therefore, although such concentrations might be attractive for anti-cancer therapies 35 or antimicrobial applications, 24 they are unlikely to be encouraging tissue regeneration. These results are consistent with previously published data, showing that curcumin inhibits cell growth or induces apoptosis in a variety of cell lines, including fibroblasts, at concentrations ranging from 10 to 100 μM.…”
Electrospun filaments represent a new generation of medical textiles with promising applications in soft tissue repair. A potential strategy to improve their design is to combine them with bioactive molecules. Curcumin, a natural compound found in turmeric, is particularly attractive for its antioxidant, anti-inflammatory, and antimicrobial properties. However, investigating the range of relevant doses of curcumin in materials designed for tissue regeneration has remained limited. In this paper, a wide range of curcumin concentrations was explored and the potential of the resulting materials for soft tissue repair applications was assessed. Polydioxanone (PDO) filaments were prepared with various amounts of curcumin: 0%, 0.001%, 0.01%, 0.1%, 1%, and 10% (weight to weight ratio). The results from the present study showed that, at low doses (≤0.1%), the addition of curcumin has no influence on the spinning process or on the physicochemical properties of the filaments, whereas higher doses lead to smaller fiber diameters and improved mechanical properties. Moreover, filaments with 0.001% and 0.01% curcumin stimulate the metabolic activity and proliferation of normal human dermal fibroblasts (NHDFs) compared with the no-filament control. However, this stimulation is not significant when compared to the control filaments (0%). Highly dosed filaments induce either the inhibition of proliferation (with 1%) or cell apoptosis (with 10%) as a result of the concentrations of curcumin found in the medium (9 and 32 μM, respectively), which are near or above the known toxicity threshold of curcumin (~10 μM). Moreover, filaments with 10% curcumin increase the catalase activity and glutathione content in NHDFs, indicating an increased production of reactive oxygen species resulting from the large concentration of curcumin. Overall, this study suggested that PDO electrospun filaments loaded with low amounts of curcumin are more promising compared with higher concentrations for stimulating tissue repair. This study also highlighted the need to explore lower concentrations when using polymers as PDO, such as those with polycaprolactone and other degradable polyesters.
“…For instance, the average diameter of PVP fibers was decreased following the incorporation of curcumin. 24 The same …”
Section: Morphology Of the Filamentsmentioning
confidence: 68%
“…[15][16][17][18] Numerous studies have proposed electrospun meshes loaded with curcumin, given the potential for health care applications. A wide range of polymers has been used, including polycaprolactone (PCL), [19][20][21] polylactic acid (PLA), 22 polylactic-co-glycolic acid, 23 polyvinylpyrrolidone (PVP), 24 silk, 25 and cellulose acetate. 26 Because of the hydrophobic nature of curcumin, the use of hydrophobic polymers such as degradable polyesters is particularly attractive to achieve a slow and prolonged release.…”
Section: Introductionmentioning
confidence: 99%
“…This result, together with the mixed results obtained from the scratch test, indicated that C1 might be at the threshold of being toxic for NHDFs. Therefore, although such concentrations might be attractive for anti-cancer therapies 35 or antimicrobial applications, 24 they are unlikely to be encouraging tissue regeneration. These results are consistent with previously published data, showing that curcumin inhibits cell growth or induces apoptosis in a variety of cell lines, including fibroblasts, at concentrations ranging from 10 to 100 μM.…”
Electrospun filaments represent a new generation of medical textiles with promising applications in soft tissue repair. A potential strategy to improve their design is to combine them with bioactive molecules. Curcumin, a natural compound found in turmeric, is particularly attractive for its antioxidant, anti-inflammatory, and antimicrobial properties. However, investigating the range of relevant doses of curcumin in materials designed for tissue regeneration has remained limited. In this paper, a wide range of curcumin concentrations was explored and the potential of the resulting materials for soft tissue repair applications was assessed. Polydioxanone (PDO) filaments were prepared with various amounts of curcumin: 0%, 0.001%, 0.01%, 0.1%, 1%, and 10% (weight to weight ratio). The results from the present study showed that, at low doses (≤0.1%), the addition of curcumin has no influence on the spinning process or on the physicochemical properties of the filaments, whereas higher doses lead to smaller fiber diameters and improved mechanical properties. Moreover, filaments with 0.001% and 0.01% curcumin stimulate the metabolic activity and proliferation of normal human dermal fibroblasts (NHDFs) compared with the no-filament control. However, this stimulation is not significant when compared to the control filaments (0%). Highly dosed filaments induce either the inhibition of proliferation (with 1%) or cell apoptosis (with 10%) as a result of the concentrations of curcumin found in the medium (9 and 32 μM, respectively), which are near or above the known toxicity threshold of curcumin (~10 μM). Moreover, filaments with 10% curcumin increase the catalase activity and glutathione content in NHDFs, indicating an increased production of reactive oxygen species resulting from the large concentration of curcumin. Overall, this study suggested that PDO electrospun filaments loaded with low amounts of curcumin are more promising compared with higher concentrations for stimulating tissue repair. This study also highlighted the need to explore lower concentrations when using polymers as PDO, such as those with polycaprolactone and other degradable polyesters.
“…Curcumin, hardly soluble in water, forms particles that are >20 μM in diameter in aqueous solutions whereas the average diameter of nano-curcumin in aqueous solutions is 100 times smaller (0.2 μM) [17]. Furthermore, nano-curcumin is more stable due to the formation of an amorphous state upon hydrogen bonding [53]. This suggests that monitoring of retinal toxicity is not required during concomitant intake of nano-curcumin; this may not hold true for standard curcumin.…”
Introduction: Curcumin has multiple biological effects including the modulation of protein homeostasis by the ubiquitin-proteasome system. The purpose of this study was to assess the in vitro cytotoxic and oxidative effects of nano-curcumin and standard curcumin and characterize their effects on proteasome regulation in retinal pigment epithelial (RPE) cells. Methods: Viability, cell cycle progression, and reactive oxygen species (ROS) production were determined after treatment with nano-curcumin or curcumin. Subsequently, the effects of nano-curcumin and curcumin on proteasome activity and the gene and protein expression of proteasome subunits PA28α, α7, β5, and β5i were assessed. Results: Nano-curcumin (5-100 μM) did not show significant cytotoxicity or anti-oxidative effects against H2O2-induced oxidative stress, whereas curcumin (≥10 μM) was cytotoxic and a potent inducer of ROS production. Both nano-curcumin and curcumin induced changes in proteasome-mediated proteolytic activity characterized by increased activity of the proteasome subunits β2 and β5i/β1 and reduced activity of β5/β1i. Likewise, nano-curcumin and curcumin affected mRNA and protein levels of household and immunoproteasome subunits. Conclusions: Nano-curcumin is less toxic to RPE cells and less prone to induce ROS production than curcumin. Both nano-curcumin and curcumin increase proteasome-mediated proteolytic activity. These results suggest that nano-curcumin may be regarded as a proteasome-modulating agent of limited cytotoxicity for RPE cells.
“…8b displays much higher tumor inhibition efficiency compared to PVP and CUR groups, which are related to the enhanced bioavailability of the CUR nanosolid dispersion.Fig. 8
a Photo of the tumor at day 32; b tumor volume x time ## denotes significant difference ( P < 0.01) against CUR group and ** denotes significant difference ( P < 0.01) against control group[Open access: (Wang et al 2015)]
…”
Melanoma is the most aggressive type of skin cancer and has very high rates of mortality. An early stage melanoma can be surgically removed, with a survival rate of 99%. This literature review intends to elucidate the possibilities to treat melanoma skin cancer using hybrid nanofibers developed by advanced electrospinning process. In this review we have shown that the enhanced permeability and retention is the basis for using nanotechnology, aiming topical drug delivery. The importance of the detection of skin cancer in the early stages is directly related to non-metastatic effects and survival rates of melanoma cells. Inhibitors of protein kinase are already available in the market for melanoma treatment and are approved by the FDA; these agents are cobimetinib, dabrafenib, ipilimumab, nivolumab, trametinib, and vemurafenib. We also report a case study involving two different approaches for targeting melanoma skin cancer therapy, namely, magnetic-based core–shell particles and electrospun mats.
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