Enhanced biodegradation of crude oil in soil by a developed bacterial consortium and indigenous plant growth promoting bacteria

Abstract

Aims This study aimed to develop an efficient, cost‐effective and eco‐friendly bacterial consortium to degrade petroleum sludge. Methods and Results Four bacterial strains belonging to genera Acinetobacter and Pseudomonas were selected to constitute three different consortia based on their initial concentration. The highest degradation rate (78%) of 1% (v/v) crude oil after 4 weeks of incubation was recorded when the concentration of biosurfactant (BS) producing isolate was high. Genes, such as alkB, almA, cyp153, pah‐rhdGN, nah, phnAC and cat23 were detected using the polymerase chain reaction method and their induction levels were optimal at pH 7·0. A crude oil sludge was artificially constituted, and its bacterial composition was investigated using 16S rRNA gene amplicon sequencing. The results showed that the soil bacterial community was dominated by plant growth‐promoting bacteria (PGPB) after crude oil treatment. Conclusions Our findings indicate the decontamination of the crude oil contaminated soil was more effective in the presence of both the constituted consortium and PGPB compared to the presence of PGPB alone. Significance and Impact of the Study This study showed that the PGPB (Taibaiella) present in petroleum uncontaminated soil can promote the soil decontamination. The addition of both efficient hydrocarbon‐degrading and BS producing bacteria is also necessary to improve the decontamination.

Genetically Modified Sugarcane Intercropping Soybean Impact on Rhizosphere Bacterial Communities and Co-occurrence Patterns

“…Therefore, the bacteria showed a higher population correlation, in other words, the rhizosphere between GM sugarcane and soybean root had higher contact levels ( Lareen et al, 2016 ). In addition, genus such as Taibaiella and Rhodanobacter that respond to GM sugarcane soybean intercropping have been identified as PGPR and play an important role in hydrocarbon degradation ( Zhang et al, 2013 ; Gutierrez, 2019 ; Diallo et al, 2021 ). The GM sugarcane–soybean intercropping pattern significantly increased the biomass these of the two crops compared with that of WT intercropping pattern ( Table 1 ); thus, intercropping of GM sugarcane and soybean will promote the growth of both species.…”

Metagenomics and Metatranscriptomics Suggest Pathways of 3-Chloroaniline Degradation in Wastewater Reactors

“…However, the family has not been directly associated with the degradation of aromatic compounds. The third genus we identified, Taibaiella , has been found in phenol-degrading microbial communities in microbial fuel cells (Shen et al ., 2020), in petroleum-contaminated soil (Diallo et al ., 2021) and in toxic landfill leachate (El-Fadel et al ., 2018), indicating tolerance to xenobiotics, if not an ability to degrade them. Gemmatimonas, OLB8 , and Taibaiella were significantly correlated with 3-CA degradation not only due to their stress tolerance, but may also have been responsible for its degradation.…”

“…Commonly found in activated sludge as a hydrolyser, it may break down complex organics (McIlroy and Nielsen, 2014; Szabó et al, 2017; Kondrotaite et al, 2022). The third genus, Taibaiella , has been found in phenol-degrading communities in microbial fuel cells (Shen et al, 2020), in petroleum-contaminated soil (Diallo et al, 2021), and in toxic landfill leachate (El-Fadel et al, 2018), indicating tolerance to xenobiotics, if not the ability to degrade them. Other ASVs that correlated (but not significantly) with 3-CA degradation included Dokdonella , Burkholderia ottowia , and two species of the order Sphingobacteriales .…”

Establishing and Optimizing a Bacterial Consortia for Effective Biodegradation of Petroleum Contaminants: Advancing Classical Microbiology via Experimental and Mathematical Approach

“…As of today, little is known about the physiology of this strain particularly, which makes it difficult to speculate the underlying reasons/mechanisms of the synergy. Nevertheless, members of Brevundimonas genus have been found to work effectively in a consortium with other strains for enhanced oil degradation [49]. Although negative correlations were found in Y2, Y3, Y4, Y5, Y6, Y7, and Y8 (PAR < 0), p-values of Y2, Y5, Y7, and Y8 were higher than 0.05, suggesting that correlations of these strains to oil biodegradation were not significant.…”