Enhanced biodegradation of hydrocarbons by Pseudomonas aeruginosa-encapsulated alginate/gellan gum microbeads

Park, Hyejoo; Kim, Hyojeon; Kim, Ga-Yeong; Lee, Mi-Young; Kim, Young; Kang, Seoktae

doi:10.1016/j.jhazmat.2020.124752

Cited by 19 publications

(8 citation statements)

References 30 publications

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“…Park et al produced Pseudomonas-aeruginosa-encapsulated alginate/gellan gum microbeads to investigate the biodegradation of diesel-contaminated groundwater. The results confirmed that encapsulation could protect microorganisms from toxic contaminants, and the activity of microorganisms could be maintained with the protective barrier of biodegradable molecules [22]. The combination of gellan gum and alginates is also applied in pharmaceutical and medical fields.…”

Section: Introductionmentioning

confidence: 53%

Stability Studies, Biodegradation Tests, and Mechanical Properties of Sodium Alginate and Gellan Gum Beads Containing Surfactant

et al. 2023

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The excessive presence of single-use plastics is rapidly degrading our natural environment on a global scale due to their inherent resistance to decomposition. Wet wipes used for personal or household purposes contribute significantly to the accumulation of plastic waste. One potential solution to address this problem involves developing eco-friendly materials that possess the ability to degrade naturally while retaining their washing capabilities. For this purpose, the beads from sodium alginate, gellan gum, and a mixture of these natural polymers containing surfactant were produced using the ionotropic gelation method. Stability studies of the beads by observing their appearance and diameter were performed after incubation in solutions of different pH values. The images showed that macroparticles were reduced in size in an acidic medium and swelled in solution of pH-neutral phosphate-buffered saline. Moreover, all the beads first swelled and then degraded in alkaline conditions. The beads based on gellan gum and combining both polymers were the least sensitive to pH changes. The compression tests revealed that the stiffness of all macroparticles decreased with the increasing pH of the solutions in which they were immersed. The studied beads were more rigid in an acidic solution than in alkaline conditions. The biodegradation of macroparticles was assessed using a respirometric method in soil and seawater. It is important to note that the macroparticles degraded more rapidly in soil than in seawater.

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Section: Introductionmentioning

confidence: 53%

Stability Studies, Biodegradation Tests, and Mechanical Properties of Sodium Alginate and Gellan Gum Beads Containing Surfactant

et al. 2023

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“…GG microspheres produced through W/O emulsion were crosslinked with calcium and nickel ions. Calcium was chosen as it is the most widely used crosslinker for GG microspheres by researchers, with proven efficacy in drug delivery [43], immobilization of cells and enzymes [32,44] and degradation of pollutants [31]. Nickel was selected since our research group had previously demonstrated this ion yielded the best capture and purification results for COMT, through a similar GG batch method [35].…”

Section: Characterization Of Gellan Gum Microspheresmentioning

confidence: 99%

“…Gellan Gum (GG) is a natural linear anionic exopolysaccharide secreted by Sphingomonas paucimobilis, which consists of four repeating carbohydrates, including two β-Dglucoses, one α-L-rhamnose, and one β-D-glucuronic acid [27,28]. Due to its properties of biocompatibility, biodegradability, hydrophilicity, mucoadhesive features and good gelling capacity, GG has found remarkable success in the fields of food [29], tissue engineering [30], bioremediation [31], biosynthesis [32] and drug delivery [33]. Indeed, GG-based materials have been shown to promote strong adsorption of small drug molecules [34].…”

Section: Introductionmentioning

confidence: 99%

Specific Six-Transmembrane Epithelial Antigen of the Prostate 1 Capture with Gellan Gum Microspheres: Design, Optimization and Integration

Batista-Silva

Gomes

Barroca-Ferreira

et al. 2023

IJMS

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This work demonstrates the potential of calcium- and nickel-crosslinked Gellan Gum (GG) microspheres to capture the Six-Transmembrane Epithelial Antigen of the Prostate 1 (STEAP1) directly from complex Komagataella pastoris mini-bioreactor lysates in a batch method. Calcium-crosslinked microspheres were applied in an ionic exchange strategy, by manipulation of pH and ionic strength, whereas nickel-crosslinked microspheres were applied in an affinity strategy, mirroring a standard immobilized metal affinity chromatography. Both formulations presented small diameters, with appreciable crosslinker content, but calcium-crosslinked microspheres were far smoother. The most promising results were obtained for the ionic strategy, wherein calcium-crosslinked GG microspheres were able to completely bind 0.1% (v/v) DM solubilized STEAP1 in lysate samples (~7 mg/mL). The target protein was eluted in a complexed state at pH 11 with 500 mM NaCl in 10 mM Tris buffer, in a single step with minimal losses. Coupling the batch clarified sample with a co-immunoprecipitation polishing step yields a sample of monomeric STEAP1 with a high degree of purity. For the first time, we demonstrate the potential of a gellan batch method to function as a clarification and primary capture method towards STEAP1, a membrane protein, simplifying and reducing the costs of standard purification workflows.

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“…Few measures espoused include the use of in situ burning, chemical dispersion, adsorption, and bioremediation. Even though bioremediation is considered to be a sustainable and eco-friendly approach, , yet the slow degradation rate and harsh environmental conditions limit the actual applications of this method. , Various bacterial genus Bacillus , Pseudomonas , Brevundimonas, Alcanivorax , and Acinetobacter have been explored for their oil biodegradation ability however their intolerance to higher concentrations of oil makes them unsuitable for in situ applications . Among the various oil-degrading bacteria, Pseudomonas genus has been well-reported to metabolize petroleum hydrocarbons of diverse chain lengths and structures.…”

Section: Introductionmentioning

confidence: 99%

“…1,3 Various bacterial genus Bacillus, Pseudomonas, Brevundimonas, Alcanivorax, and Acinetobacter have been explored for their oil biodegradation ability however their intolerance to higher concentrations of oil makes them unsuitable for in situ applications. 4 Among the various oildegrading bacteria, Pseudomonas genus has been well-reported to metabolize petroleum hydrocarbons of diverse chain lengths and structures. In a study, P. aeruginosa D5D1 exhibited 24.6, 38.3, and 43.6% biodegradation ability for C12−16, C17−20, and C21−25 hydrocarbons contents, respectively, in diesel oil (10% v/v, initial concentration).…”

Section: Introductionmentioning

confidence: 99%

Hydrophobic Surface Induced Biosorption and Microbial Ex Situ Remediation of Oil-Contaminated Sites

Sharma

Pandey

2021

Ind. Eng. Chem. Res.

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Microbial bioremediation of spilled oil is the most sustainable and eco-friendly approach, yet the slower biodegradation rate remains a challenge for researchers. An integrated strategy of biodegradation along with a faster removal method like adsorption is one of the options available to address this challenge. In this study, we have explored the surface-induced biosorption coupled microbial biodegradation approach for effective remediation. Low-cost bagasse was used as a biosorbent and its surface hydrophobicity was improved by forming octyl self-assembled monolayers to enhance the surface-oil interactions (adsorption). Pseudomonas aeruginosa was exploited for biodegradation because of its known oil degradation and biosurfactant production abilities. An enhanced oil adsorption capacity of 6.9 ± 0.3 g/g was obtained after surface modification of the bagasse. The rate of adsorption was 2.5 fold higher as compared to unmodified bagasse. A kinetic model is proposed for the integrated process, which agreed to simultaneous biosorption and biodegradation. The microbial oil biodegradation rate was improved by 17 fold in the case of the integrated process. This integrated approach was found to synergistically improve the overall remediation of crude oil, which was also correlated with alkane hydroxylase activity and biosurfactant production. Preadsorbed oil on hydrophobic bagasse improved oil availability for the adhered microbes. Further, the synthesis of biosurfactant amended the biodegradation process. Hence, in the foreseeable future, hydrophobic biosorbents with excellent adsorption capacity, stability, and reusability shall be potential carriers for the bioremediation of contaminated sites.

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Enhanced biodegradation of hydrocarbons by Pseudomonas aeruginosa-encapsulated alginate/gellan gum microbeads

Cited by 19 publications

References 30 publications

Stability Studies, Biodegradation Tests, and Mechanical Properties of Sodium Alginate and Gellan Gum Beads Containing Surfactant

Stability Studies, Biodegradation Tests, and Mechanical Properties of Sodium Alginate and Gellan Gum Beads Containing Surfactant

Specific Six-Transmembrane Epithelial Antigen of the Prostate 1 Capture with Gellan Gum Microspheres: Design, Optimization and Integration

Hydrophobic Surface Induced Biosorption and Microbial Ex Situ Remediation of Oil-Contaminated Sites

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