Enhanced Biodegradation of Phenylurea Herbicides by Ochrobactrum anthrophi CD3 Assessment of Its Feasibility in Diuron-Contaminated Soils

Lara-Moreno, Alba; Morillo, E.; Villaverde, Jaime

doi:10.3390/ijerph19031365

Cited by 13 publications

(5 citation statements)

References 54 publications

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“…Another possibility could be that RAMEB was also acting as a biostimulant for the soil microbiota activity. This fact has been previously demonstrated for other CDs [25,30,[63][64][65]. Although RAMEB is considered a poorly biodegradable CD [66], Fava et al [67] observed that aerobic microorganisms isolated from polychlorinated-biphenyls-contaminated soil were able to metabolise RAMEB as only carbon and energy source.…”

Section: Discussionmentioning

confidence: 59%

Chlorpyrifos Removal in an Artificially Contaminated Soil Using Novel Bacterial Strains and Cyclodextrin. Evaluation of Its Effectiveness by Ecotoxicity Studies

et al. 2022

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The removal of chlorpyrifos (CLP) from the environment is a matter of general interest, because it is one of the most widely used insecticides in the world but presents a high toxicity and persistence in the environment. Biological strategies are considered as a good option to remediate different environmental compartments. Assisted natural attenuation was used to find the ability of different kinds of soils to mineralise CLP. In this way, two soils showed the capacity to degrade CLP (R and LL up to 47.3% and 61.4% after 100 d, respectively). Thus, two CLP-degrading strains, Bacillus megaterium CCLP1 and Bacillus safensis CCLP2 were isolated from them, showing the capacity to degrade up to 99.1 and 98.9% of CLP in a solution with an initial concentration of 10 mg L−1 after 60 d. Different strategies were considered for increasing the effectiveness of soil bioremediation: (i) biostimulation, using a nutrients solution (NS); (ii) bioaugmentation, using B. megaterium CCLP1 or B. safensis CCLP2; (iii) bioavailability enhancement, using randomly methylated β-cyclodextrin (RAMEB), a biodegradable compound. When bioaugmentation and RAMEB were jointly inoculated and applied, the best biodegradation results were achieved (around 70%). At the end of the biodegradation assay, a toxicity test was used to check the final state of the bioremediated soil, observing that when the degrading strains studied were individually inoculated into the soil, the toxicity was reduced to undetectable levels.

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Section: Discussionmentioning

confidence: 59%

Chlorpyrifos Removal in an Artificially Contaminated Soil Using Novel Bacterial Strains and Cyclodextrin. Evaluation of Its Effectiveness by Ecotoxicity Studies

et al. 2022

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“…Some bacteria capable of degrading diuron and linuron to mono- or dichloroaniline, such as 34DCA and 4-chloroaniline, have been reported [ 5 , 6 , 7 ]. Sphingomonas sp.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the characterization of mono-and dichloroaniline biodegradation is important for the remediation of environments contaminated by phenylurea herbicides. Some bacteria capable of degrading diuron and linuron to mono-or dichloroaniline, such as 34DCA and 4-chloroaniline, have been reported [5][6][7]. Sphingomonas sp.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the 3,4-Dichloroaniline Degradation Gene Cluster in Acinetobacter soli GFJ2

Gibu,

Kasai,

Sato

et al. 2024

Microorganisms

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3,4-Dichloroaniline (34DCA), a major metabolite of phenylurea herbicides, causes environmental contamination owing to its toxicity and recalcitrant properties. Acinetobacter soli strain GFJ2, isolated from soil potentially contaminated with herbicides, can degrade 34DCA. This study aimed to identify and characterize the 34DCA degradation gene cluster responsible for the conversion of 34DCA to 4,5-dichlorocatechol in the strain GFJ2. Genome analysis revealed one chromosome and seven plasmids in GFJ2, comprising 21, 75, and 3309 copies of rRNA, 75 tRNA, and protein-encoding genes, respectively. A gene cluster responsible for 34DCA degradation was identified, comprising dcdA, dcdB, and dcdC, which encode dioxygenase, flavin reductase, and aldehyde dehydrogenase, respectively. Transcriptional analysis indicated that this gene cluster is constructed as an operon, induced during 34DCA utilization. The heterologous expression of dcdA and dcdB in Escherichia coli confirmed their activity in degrading 34DCA to an intermediate metabolite, converted to 4,5-dichlorocatechol via a reaction involving the dcdC gene product, suggesting their involvement in 34DCA conversion to 4,5-dichlorocatechol. Deletion mutants of dcdA and dcdB lost 34DCA degradation ability, confirming their importance in 34DCA utilization in GFJ2. This study provides insights into the genetic mechanisms of 34DCA degradation by GFJ2, with potential applications in the bioremediation of environments contaminated by phenylurea herbicides.

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“…Hathout et al, ( 2022 ) data revealed that agricultural soil samples from Egypt governorates were highly contaminated (from 10 to 80%) by different pesticide residues. Many researchers have reported that IPU has deleterious effects on microbial communities and is carcinogenic for animals and humans (Vallotton et al 2009 ; Alba et al 2022 ). The IPU is documented to induce oxidative stress that results in the damage of proteins, lipids, and other cellular components, thus inhibiting growth or leading to organism death (Rutherford and Krieger-Liszkay 2001 ).…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of isoproturon by Escherichia coli expressing a Pseudomonas putida catechol 1,2-dioxygenase gene

Elarabi,

Abdelhadi,

Nassrallah

et al. 2023

AMB Expr

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The phenylurea herbicides are persistent in soil and water, necessitating the creation of methods for removing them from the environment. This study aimed to examine the soil microbial diversity, searching for local bacterial isolates able to efficiently degrade the phenylurea herbicide isoproturon, 3-(4-isopropylphenyl)-1, 1-dimethylurea (IPU). The best isolates able to effectively degrade IPU were selected, characterized, and identified as Pseudomonas putida and Acinetobacter johnsonii. The catechol 1, 2-dioxygenase enzyme's catA gene was amplified, cloned, and expressed in E. coli M15. The Expressed E. coli showed high degradation efficiency (44.80%) as analyzed by HPLC after 15 days of inoculation in comparison to P. putida (21.60%). The expression of the catA gene in P. putida and expressed E. coli was measured using quantitative polymerase chain reaction (qPCR). The results displayed a significant increase in the mRNA levels of the catA gene by increasing the incubation time with IPU. Hydrophilic interaction chromatography (HILIC) mass spectrometry analysis revealed that three intermediate metabolites, 1-(4-isopropylphenyl)-3-methylurea (MDIPU), 4-Isopropylaniline (4-IA) and 1-(4-isopropylphenyl) urea (DDIPU) were generated by both P. putida and expressed E. coli. In addition, IPU-induced catA activity was detected in both P. putida and expressed E. coli. The supernatant of both P. putida and expressed E. coli had a significant influence on weed growth. The study clearly exhibited that P. putida and expressed E. coli were capable of metabolizing IPU influentially and thus could be utilized for bioremediation and biodegradation technology development.

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Enhanced Biodegradation of Phenylurea Herbicides by Ochrobactrum anthrophi CD3 Assessment of Its Feasibility in Diuron-Contaminated Soils

Cited by 13 publications

References 54 publications

Chlorpyrifos Removal in an Artificially Contaminated Soil Using Novel Bacterial Strains and Cyclodextrin. Evaluation of Its Effectiveness by Ecotoxicity Studies

Chlorpyrifos Removal in an Artificially Contaminated Soil Using Novel Bacterial Strains and Cyclodextrin. Evaluation of Its Effectiveness by Ecotoxicity Studies

Characterization of the 3,4-Dichloroaniline Degradation Gene Cluster in Acinetobacter soli GFJ2

Biodegradation of isoproturon by Escherichia coli expressing a Pseudomonas putida catechol 1,2-dioxygenase gene

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