Enhanced biological properties of collagen/chitosan-coated poly(ε-caprolactone) scaffold by surface modification with GHK-Cu peptide and 58S bioglass

Abstract

Bioactive glasses and peptides have shown promising results in improving wound healing and skin repair. The present study explores the effectiveness of surface modification of collagen/chitosan-coated electrospun poly(ε-caprolactone) scaffold with 58S bioactive glass or GHK-Cu peptide. To coat scaffolds with the bioactive glass, we prepared suspensions of silanized bioactive glass powder with three different concentrations and the scaffolds were pipetted with suspensions. Similarly, GHK-Cu-coated scaffolds were prepared by pipetting adequate amount of 1-mM solution of peptide (in milli-Q) on the surface of scaffolds. ATR-FTIR spectroscopy indicated the successful modification of collagen/chitosan-coated electrospun poly(εcaprolactone) scaffold with bioactive glass and GHK-Cu. Microstructural investigations and in vitro studies such as cell adhesion, cell viability and antibacterial assay were performed. All samples demonstrated desirable cell attachment. Compared to poly(ε-caprolactone)/collagen/chitosan, the cell proliferation of GHK-Cu and bioactive glass-coated (concentrations of 0.01 and 0.1) scaffolds increased significantly at days 3 and 7, respectively. Poly(ε-caprolactone)/collagen/chitosan-uncoated scaffold and scaffolds coated with GHK-Cu and bioactive glass revealed desirable antibacterial properties but the antibacterial activity of GHK-Cu-coated sample turned out to be superior. These findings indicated that biological properties of collagen/ chitosan-coated synthetic polymer could be improved by GHK-Cu and bioactive glass.

Bioactive glass-based fibrous wound dressings

“…Inorganic BG fibers are trivially much stiffer than skin but the BG-incorporated polymer (e.g. BG/PCL [ 137 ]) fibers can be so developed to properly match the skin’s elastic modulus. These properties can be adjusted in BG/polymer nanofibers to match that of the skin tissue through proper selection of polymer matrix, the quantity of the BG filler, and surface chemistry of the filler (in the presence/absence of coupling agents), which affects the physicochemical interaction (bonding) between the BG particles and the polymer.…”

Collagen/Chitosan Functionalization of Complex 3D Structures Fabricated by Laser Direct Writing via Two-Photon Polymerization for Enhanced Osteogenesis

“…A major point is related to the chemical composition of the Col/CT coating that may account, at least to a certain extent, for samples’ wettability. In this regard, we argue that the dip coating technique used for the functionalization our structures is generally recognized as suitable for coating complex shapes and curved parts (like those in the 3D geometries presented in this study) that could not be coated by any technique [ 27 ]. Moreover, the chemical composition of other complex structures functionalized by dip coating in Col and CT has been extensively investigated by Fourier transformed infrared spectroscopy, for both individual materials as well as in blends, and therefore the results could be easily extrapolated to our experimental conditions [ 28 ].…”

Antibacterial multicomponent electrospun nanofibrous mat through the synergistic effect of biopolymers

“…50,51 Because of its good cell permeability, collagen incorporated nanofibrous mat can be employed in wound areas for repairing and regeneration of damaged tissue. 52,53 Collagen is a protein polymer that exhibits good properties that enable the culture of muscle and lung cells, fibroblasts, spinal ganglion cell line. If repair, restoration of the structure of disrupted injured tissues is needed, collagen acts as an active bio-compound.…”