Enhanced biomass and phycocyanin production of Arthrospira (Spirulina) platensis by a cultivation management strategy: Light intensity and cell concentration

Chaiklahan, Ratana; Chirasuwan, Nattayaporn; Srinorasing, Thanyarat; Attasat, Shewin; Nopharatana, Annop; Bunnag, Boosya

doi:10.1016/j.biortech.2021.126077

Cited by 60 publications

(24 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The biomass productivity and biochemical composition of Spirulina biomasses are highly dependent on the adjusted cultivation conditions, such as temperature [ 9 ], light quality (spectrum) [ 10 , 11 , 12 ] and quantity (intensity) [ 13 , 14 ], and the availability of nutrients [ 15 ]. Spirulina is commonly cultivated in large-scale open raceway ponds using natural sunlight.…”

Section: Introductionmentioning

confidence: 99%

Modeling and Optimizing the Effect of Light Color, Sodium Chloride and Glucose Concentration on Biomass Production and the Quality of Arthrospira platensis Using Response Surface Methodology (RSM)

Nosratimovafagh

Fereidouni

Krujatz

2022

Life

View full text Add to dashboard Cite

Arthrospira platensis (Spirulina) biomass is a valuable source of sustainable proteins, and the basis for new food and feed products. State-of-the-art production of Spirulina biomass in open pond systems only allows limited control of essential process parameters, such as light color, salinity control, or mixotrophic growth, due to the high risk of contaminations. Closed photobioreactors offer a highly controllable system to optimize all process parameters affecting Spirulina biomass production (quantity) and biomass composition (quality). However, a comprehensive analysis of the impact of light color, salinity effects, and mixotrophic growth modes of Spirulina biomass production has not been performed yet. In this study, Response Surface Methodology (RSM) was employed to develop statistical models, and define optimal mixotrophic process conditions yielding maximum quantitative biomass productivity and high-quality biomass composition related to cellular protein and phycocyanin content. The individual and interaction effects of 0, 5, 15, and 30 g/L of sodium chloride (S), and 0, 1.5, 2, and 2.5 g/L of glucose (G) in three costume-made LED panels (L) where the dominant color was white (W), red (R), and yellow (Y) were investigated in a full factorial design. Spirulina was cultivated in 200 mL cell culture flasks in different treatments, and data were collected at the end of the log growth phase. The lack-of-fit test showed that the cubic model was the most suitable to predict the biomass concentration and protein content, and the two-factor interaction (2FI) was preferred to predict the cellular phycocyanin content (p > 0.05). The reduced models were produced by excluding insignificant terms (p > 0.05). The experimental validation of the RSM optimization showed that the highest biomass concentration (1.09, 1.08, and 0.85 g/L), with improved phycocyanin content of 82.27, 59.47, 107 mg/g, and protein content of 46.18, 39.76, 53.16%, was obtained under the process parameter configuration WL4.28S2.5G, RL10.63S1.33G, and YL1.00S0.88G, respectively.

show abstract

Section: Introductionmentioning

confidence: 99%

Modeling and Optimizing the Effect of Light Color, Sodium Chloride and Glucose Concentration on Biomass Production and the Quality of Arthrospira platensis Using Response Surface Methodology (RSM)

Nosratimovafagh

Fereidouni

Krujatz

2022

Life

View full text Add to dashboard Cite

show abstract

“…For example, the biomass with high phycocyanin content can be specially used for the development of natural food colors or other phycocyanin products, while the one containing high carbohydrates content has potential as a source for biofuel generation or active polysaccharides production. However, even though it was associated with genetic characteristics of algal strains, the substances contained in the Arthrospira biomass were influenced by factors such as amounts of nutrients (fertilizers), temperature, and light intensity [ 13 , 15 , 20 , 34 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

“…The maximum biomass productivity occurred on day 25, with 21.97 g m −2 d −1 , but the average biomass productivity (3.96 g m −2 d −1 ) was much lower than previously reported [ 16 , 38 ]. The medium components and environmental factors largely affect the growth [ 13 , 15 , 20 , 34 , 35 ]. The growth, shown as biomass production and productivity in our study, was significantly lower than the results from those studies mentioned above, which was mainly attributed to the following reasons.…”

Section: Discussionmentioning

confidence: 99%

“…Consequently, the biochemical composition plays a key role in the application directions of the Arthrospira biomass. However, the growth and biochemical composition of Arthrospira were influenced by many factors, such as nutrients, temperature, light, and salinity [ 13 , 14 , 15 ]. Strategies for regulating these factors have been developed to increase the accumulation of high-value products in Arthrospira for different application purposes [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Growth and Biochemical Composition Characteristics of Arthrospira platensis Induced by Simultaneous Nitrogen Deficiency and Seawater-Supplemented Medium in an Outdoor Raceway Pond in Winter

et al. 2021

Foods

View full text Add to dashboard Cite

Arthrospira platensis, a well-known cyanobacterium, is widely applied not only in human and animal nutrition but also in cosmetics for its high amounts of active products. The biochemical composition plays a key role in the application performance of the Arthrospira biomass. The present study aimed to evaluate the growth and biochemical composition characteristics of A. platensis, cultured with a nitrogen-free and seawater-supplemented medium in an outdoor raceway pond in winter. The results showed that the biomass yield could achieve 222.42 g m−2, and the carbohydrate content increased by 247% at the end of the culture period (26 d), compared with that of the starter culture. The daily and annual areal productivities were 3.96 g m−2 d−1 and 14.44 ton ha−1 yr−1 for biomass and 2.88 g m−2 d−1 and 10.53 ton ha−1 yr−1 for carbohydrates, respectively. On the contrary, a profound reduction was observed in protein, lipid, and pigment contents. Glucose, the main monosaccharide in the A. platensis biomass, increased from 77.81% to 93.75% of total monosaccharides. Based on these results, large-scale production of carbohydrate-rich A. platensis biomass was achieved via a low-cost culture, involving simultaneous nitrogen deficiency and supplementary seawater in winter.

show abstract

“…A. platensis, together with A. maxima, is the main source of commercial PC. This organism can produce about 20% of PC (with PC/APC ratio usually around 3), respect to the biomass dry weight (d.w.) (de Morais et al, 2018;Kannaujiya et al, 2017;Chaiklahan et al, 2022). However, PC commonly represents about 6-15% of the biomass d.w., especially when produced in large scale biomass cultivation ponds (Chaiklahan et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

High purity grade phycocyanin recovery by decupling cell lysis from the pigment extraction – an innovative approach

Lauceri¹,

Cavone²,

Zittelli³

et al. 2022

Preprint

View full text Add to dashboard Cite

Phycocyanin, a phycobiliprotein, is one of the few natural blue pigments available as food colorant and it is largely used in food industry and in cosmetics. We have devised an innovative two-step extraction process which allowed to obtain bright blue phycocyanin crude extracts with high purity grade P (within 2.5 and 3.5) directly from fresh biomass of Arthrospira platensis Gomont 1892 (commonly named Spirulina). We found out and for the first time exploited ammonium sulphate capability to minimize the release of water soluble phycobiliproteins in aqueous medium during ultrasound-assisted cell lysis/purification phase. The conventional sequence which is, extraction followed by purification, was reversed. The extraction phase was 2 decoupled from biomass cell lysis. Cell lysis, accomplished by ultrasonication in ammonium sulphate solution, was merged with purification in a single step, before the pigment extraction/recovering phase. The process was entirely carried out in aqueous solutions. No downstream purification was required to obtain products suitable for the most common phycocyanin applications (i.e., foods, nutraceuticals, cosmetics). Production time, hours instead of days, was reduced to the advantage of the product quality. The process has the great advantages of (1) direct use of extracting solutions that cannot be used in the ordinary ultrasound-assisted extraction of phycocyanin (because of the extensive simultaneous extraction of contaminant molecules), (2) gain of high commercial value phycocyanin due to the elevated purity grade and (3) direct production of highly concentrated bright blue pigment crude extracts (up to about 5 mg mL -1 ) immediately in hand to the market.

show abstract

Enhanced biomass and phycocyanin production of Arthrospira (Spirulina) platensis by a cultivation management strategy: Light intensity and cell concentration

Cited by 60 publications

References 33 publications

Modeling and Optimizing the Effect of Light Color, Sodium Chloride and Glucose Concentration on Biomass Production and the Quality of Arthrospira platensis Using Response Surface Methodology (RSM)

Modeling and Optimizing the Effect of Light Color, Sodium Chloride and Glucose Concentration on Biomass Production and the Quality of Arthrospira platensis Using Response Surface Methodology (RSM)

Growth and Biochemical Composition Characteristics of Arthrospira platensis Induced by Simultaneous Nitrogen Deficiency and Seawater-Supplemented Medium in an Outdoor Raceway Pond in Winter

High purity grade phycocyanin recovery by decupling cell lysis from the pigment extraction – an innovative approach

Contact Info

Product

Resources

About