Enhanced biomineralization and protein adsorption capacity of 3D chitosan/hydroxyapatite biomimetic scaffolds applied for bone-tissue engineering

Nga, Nguyen Kim; Tam, Lai Thi Thanh; Hà, Nguyễn Thu; Pham, Hung Viet; Huy, Tran Quang

doi:10.1039/d0ra09432c

Cited by 68 publications

(41 citation statements)

References 57 publications

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“…Similar findings were observed for nZnO-loaded hydrogels showing increased BSA adsorption on hydrogel samples by increasing the amount of nZnO, attributed to the reduced hydrophilicity compared to the hydrogel matrices (PVA and CS) [43]. Likewise, addition of hydroxyapatite nano-crystallites modified the characteristics of the chitosan scaffold surfaces (e.g., chemical composition, electric charge and morphology), thereby greatly increasing the quantity of active sites on the composite scaffolds for possible interactions with proteins [88].…”

Section: Protein Adsorptionsupporting

confidence: 72%

Glibenclamide Nanocrystal-Loaded Bioactive Polymeric Scaffolds for Skin Regeneration: In Vitro Characterization and Preclinical Evaluation

et al. 2021

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Skin restoration following full-thickness injury poses significant clinical challenges including inflammation and scarring. Medicated scaffolds formulated from natural bioactive polymers present an attractive platform for promoting wound healing. Glibenclamide was formulated in collagen/chitosan composite scaffolds to fulfill this aim. Glibenclamide was forged into nanocrystals with optimized colloidal properties (particle size of 352.2 nm, and polydispersity index of 0.29) using Kolliphor as a stabilizer to allow loading into the hydrophilic polymeric matrix. Scaffolds were prepared by the freeze drying method using different total polymer contents (3–6%) and collagen/chitosan ratios (0.25–2). A total polymer content of 3% at a collagen/chitosan ratio of 2:1 (SCGL3-2) was selected based on the results of in vitro characterization including the swelling index (1095.21), porosity (94.08%), mechanical strength, rate of degradation and in vitro drug release. SCGL3-2 was shown to be hemocompatible based on the results of protein binding, blood clotting and percentage hemolysis assays. In vitro cell culture studies on HSF cells demonstrated the biocompatibility of nanocrystals and SCGL3-2. In vivo studies on a rat model of a full-thickness wound presented rapid closure with enhanced histological and immunohistochemical parameters, revealing the success of the scaffold in reducing inflammation and promoting wound healing without scar formation. Hence, SCGL3-2 could be considered a potential dermal substitute for skin regeneration.

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Section: Protein Adsorptionsupporting

confidence: 72%

Glibenclamide Nanocrystal-Loaded Bioactive Polymeric Scaffolds for Skin Regeneration: In Vitro Characterization and Preclinical Evaluation

et al. 2021

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“…The higher protein adsorption capability of the three‐dimensional‐PLA/Gel/Mg‐nHAp scaffolds might be due to their suitable porous architecture as well as the presence of organic and inorganic components mimicking the biological bone matrix and apatite. [ 36 ] The photographic images of PLA, PLA/Gel and PLA/Gel/Mg‐nHAp scaffolds are shown in Figure S6.…”

Section: Resultsmentioning

confidence: 99%

Three‐dimensional‐poly(lactic acid) scaffolds coated with gelatin/magnesium‐doped nano‐hydroxyapatite for bone tissue engineering

Swetha

Balagangadharan

Lavanya

et al. 2021

Biotechnology Journal

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Background Treatment of critical‐sized bone defects has progressively evolved over the years from metallic implants to more ingenious three‐dimensional‐based scaffolds. The use of three‐dimensional scaffolds for bone regeneration from biodegradable polymers like poly(lactic acid) (PLA) is gaining popularity. Scaffolds with surface functionalization using gelatin (Gel) have the advantages of biocompatibility and cell adhesion. Nano‐hydroxyapatite (nHAp) is one of the most promising implant materials utilized in orthopaedics. The osteogenic potential of the nHAp can be improved by the substitution of magnesium (Mg) ions onto the crystal lattice of nHAp. Thus, the goal of this work was to make three‐dimensional‐PLA scaffolds covered with Gel/Mg‐nHAp for osteogenic effect. Methods and results The designed three‐dimensional‐PLA/Gel/Mg‐nHAp scaffolds were attributed to various characterizations for the examination of their physicochemical, mechanical properties, cyto‐compatibility, and biodegradability as well as their ability to promote osteogenesis in vitro. Mouse mesenchymal stem cells (mMSCs) were cytocompatible with these scaffolds. The osteogenic potential of three‐dimensional‐PLA/Gel/Mg‐nHAp scaffolds employing mMSCs was validated at the cellular and molecular levels. The three‐dimensional‐PLA/Gel/Mg‐nHAp scaffolds stimulated the differentiation of mMSCs towards osteoblastic lineage. Conclusion Based on these findings, we suggest that the three‐dimensional‐PLA/Gel/Mg‐nHAp scaffolds' osteogenic capability may be advantageous in the mending of bone defects in orthopedic applications.

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“…Compared with chemically synthesized HAP, 52 the intensity of the diffraction peaks of biogenic HAP was relatively low, which could be attributed to the existence of biomolecules in biogenic HAP, and the facile biological synthesis parameters such as the pH and temperature. 53–56 SEM imaging showed that the biogenic HAP formed disc-shaped nanoparticles with a diameter range of 50–100 nm (Fig. 1b).…”

Section: Resultsmentioning

confidence: 99%

Cd stress alleviation in mung-bean seedlings with biogenic hydroxyapatite nanoparticles as ecofriendly remediation agents

Shen

Zhang

et al. 2022

Environ. Sci.: Nano

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Enhanced biomineralization and protein adsorption capacity of 3D chitosan/hydroxyapatite biomimetic scaffolds applied for bone-tissue engineering

Abstract: This work presents the enhanced biomineralization and protein adsorption capacity of 3D chitosan/hydroxyapatite (CS/HAp) biomimetic scaffolds synthesized from natural sources applied for bone-tissue engineering (BTE).

Cited by 68 publications

References 57 publications

Glibenclamide Nanocrystal-Loaded Bioactive Polymeric Scaffolds for Skin Regeneration: In Vitro Characterization and Preclinical Evaluation

Glibenclamide Nanocrystal-Loaded Bioactive Polymeric Scaffolds for Skin Regeneration: In Vitro Characterization and Preclinical Evaluation

Three‐dimensional‐poly(lactic acid) scaffolds coated with gelatin/magnesium‐doped nano‐hydroxyapatite for bone tissue engineering

Cd stress alleviation in mung-bean seedlings with biogenic hydroxyapatite nanoparticles as ecofriendly remediation agents

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