Enhanced bone healing using collagen-hydroxyapatite scaffold implantation in the treatment of a large multiloculated mandibular aneurysmal bone cyst in a thoroughbred filly

Abstract

An unmet need remains for a bone graft substitute material that is biocompatible, biodegradable and capable of promoting osteogenesis safely in vivo. The aim of this study was to investigate the use of a novel collagen–hydroxyapatite (CHA) bone graft substitute in the clinical treatment of a mandibular bone cyst in a young horse and to assess its potential to enhance repair of the affected bone. A 2 year‐old thoroughbred filly, presenting with a multilobulated aneurysmal bone cyst, was treated using the CHA scaffold. Post‐operative clinical follow‐up was carried out at 2 weeks and 3, 6 and 14 months. Cortical thickening in the affected area was observed from computed tomography (CT) examination as early as 3 months post‐surgery. At 14 months, reduced enlargement of the operated mandible was observed, with no fluid‐filled area. The expansile cavity was occupied by moderately dense mineralized tissue and fat and the compact bone was remodelled, with a clearer definition between cortex and medulla observed. This report demonstrates the promotion of enhanced bone repair following application of the CHA scaffold material in this craniomaxillofacial indication, and thus the potential of this material for translation to human applications. Copyright © 2015 John Wiley & Sons, Ltd.

Rapid healing of a critical‐sized bone defect using a collagen‐hydroxyapatite scaffold to facilitate low dose, combinatorial growth factor delivery

“…A cell‐free (prior to implantation), biomimetic, and osteoconductive CHA scaffold was chosen as the optimal matrix to promote bone tissue regeneration due to its well‐documented efficacy in the healing of bone defects in vivo (David et al, ; Gleeson et al, ; Lyons et al, ). The use of this scaffold offered an alternative approach to two commonly employed methodologies in BTE—namely, the use of controlled delivery technologies and the use of supraphysiological protein doses.…”

Functionalization of a Collagen–Hydroxyapatite Scaffold with Osteostatin to Facilitate Enhanced Bone Regeneration

“…In addition, one variant has demonstrated clinical success in a large equine mandibular bone cyst in a recent study. [ 17,18 ] One way of further enhancing the regenerative properties of scaffolds is to functionalize them for the controlled delivery of therapeutic molecules with pro-anabolic or antiresorptive capacity. Related to this, our research group has evaluated the incorporation of drug eluting carriers in the form of thermosensitive liposomes or polymeric microparticles embedded within the scaffold structure in order to protect their sensitive therapeutic cargo.…”

Growth plate extracellular matrix-derived scaffolds for large bone defect healing

“…Porous scaffolds produced using collagen and hydroxyapatite, the two main constituents of bone, have been shown to encourage osteogenesis of MSCs in vitro and can support bone regeneration in vivo (Calabrese et al, 2016;Chen et al, 2015;Cunniffe et al, 2010;Cunniffe and O'Brien, 2011;David et al, 2015;Gleeson et al, GM Cunniffe et al Growth plate ECM scaffold for bone repair 2010; Prosecka et al, 2015;Villa et al, 2015). Ideally scaffolds have an open interconnected pore structure, with pore sizes in the range of 85-325 µm best supporting osteogenesis (Karageorgiou and Kaplan, 2005;Murphy et al, 2010;O'Brien et al, 2007).…”