Enhanced brightness and photostability dye-sensitized Nd-doped rare earth nanocomplex for in vivo NIR-IIb vascular and orthotopic tumor imaging

Abstract

Rare-earth doped nanoparticles (RENPs) have shown promise in biomedical imaging, particularly in the NIR-IIb region, due to their deep tissue penetration and minimal light scattering. However, challenges such as low extinction coefficients, narrow excitation spectra, and susceptibility to quenching in aqueous environments limit their effectiveness. To overcome these obstacles, we developed a novel dye-sensitized, onion-like Nd-doped RENP nanocomplex designed to enhance NIR-IIb imaging performance. The onion-like Nd-RENP nanocomplex significantly boosts emission intensity at 1525 nm within the NIR-IIb range by reducing quenching and improving spectral overlap. The integration of an IR783-containing micellar layer further stabilizes the NIR dye, protecting it from quenching and reducing photobleaching. In vivo imaging studies demonstrated a 75-fold increase in luminance and a 9-fold improvement in photostability compared to free NIR dyes in aqueous solutions. Time-dependent in vivo studies confirmed the nanocomplex's capability for prolonged vascular and tumor imaging, maintaining high-resolution images for over an hour. Additionally, the nanocomplex supported successful 3D imaging reconstruction of biological tissues. The dye-sensitized onion-like Nd-RENP nanocomplex presents a significant advancement in NIR-IIb imaging, providing enhanced brightness and photostability. Its ability to maintain clear and stable imaging over extended periods suggests potential applications in dynamic vascular and tumor-targeted imaging. This innovation holds promise for future biomedical imaging technologies, particularly in areas requiring high-resolution and long-duration monitoring.