Enhanced C-5 sugar production from pine needle waste biomass using Bacillus sp. XPB-11 mutant and its biotransformation to bioethanol

Upcycling of tetra pack waste cellulose into reducing sugars for bioethanol production using Saccharomyces cerevisiae

“…Bioethanol offers the potential to mitigate fuel resource depletion, provide a renewable energy source, and reduce harmful emissions compared to conventional fossil fuels (Dwivedi et al, 2022). Cellulases, enzymes capable of hydrolyzing cellulose into glucose, are essential for utilizing tetra pack waste to produce bioethanol (Kucharska et al, 2018;Suri et al, 2021). The ndings of this research offer a sustainable solution for managing tetra pack waste, transforming it into bioethanol, thereby reducing environmental waste and providing a clean, renewable energy source to safeguard the environment.…”

“…The production of cellulase from Bacillus sp. RL-07 carried out by following the methodology outlined by Suri et al (2021). Initially, a loopful of Bacillus sp.…”

“…Cellulase activity was determined using the Dinitrosalicylic acid assay (DNSA), as per the modi ed method described by Suri et al (2021). Initially, 0.5 ml of cellulase was diluted with an equal volume of citrate buffer (0.05 M, pH 4.8).…”

Strategy of Coniferous Needle Biorefinery into Value-Added Products to Implement Circular Bioeconomy Concepts in Forestry Side Stream Utilization

“…Coniferous needles have also been investigated for bioethanol production through enzymatic or chemical hydrolysis. However, the yield obtained cannot be considered as high (bioethanol yield of 3.89%, compared to wheat at 39% and corn at 41%) due to the low cellulose content, and other components of the needles appear to inhibit the fermentation process [34,35]. Moreover, coniferous needles have been used as fibres in combination with synthetic (urea-formaldehyde, resorcinol-formaldehyde, and others) and biodegradable (poly lactic acid and others) polymers or concrete to develop composites with enhanced mechanical properties [36,37].…”

“…Coniferous tree greenery has been used for energy production, and gasification is considered a promising approach for generating bioenergy [31]. Another thermochemical processing approach, pyrolysis, has been proposed to produce biochar from coniferous needles, which can be used for energy production, in agriculture, and as a sorbent [32][33][34]. Coniferous needles have also been investigated for bioethanol production through enzymatic or chemical hydrolysis.…”