Enhanced Cellular Mobility Guided by TiO2 Nanotube Surfaces

Brammer, Karla S.; Oh, Seunghan; Gallagher, J; Jin, Sung‐Ho

doi:10.1021/nl072572o

Cited by 263 publications

(243 citation statements)

References 22 publications

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“…We prepared nominally 30-, 50-, 70-, and 100-nm TiO 2 nanotubes on Ti substrates by anodization (25), as depicted in Fig. 1, to determine how the size of the nanotubes would play a role in the cellular response and differentiation of hMSCs [detailed experimental descriptions can be found in Materials and Methods and in supporting information (SI) Materials and Methods].…”

Section: Resultsmentioning

confidence: 99%

“…Fabrication of the nanostructured titanium dioxide (TiO 2 ) nanotube arrays has been a primary subject of investigation lately because of the wide range of TiO 2 applications in the fields of solar cells (13)(14)(15)(16), photocatalysis (17)(18)(19), photoelectrolysis (20), sensors (21,22), and biomaterials (23)(24)(25). The presence of a vertically aligned TiO 2 nanotube surface on Ti foils had a critical effect that improved the proliferation and mineralization of osteoblasts (24) and enhanced the mobility, vasodilation, and monolayer formation of endothelial cells (25) because of the unique nanotopographical features and high biocompatibility of the TiO 2 nanotube surface.…”

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confidence: 99%

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Stem cell fate dictated solely by altered nanotube dimension

Oh¹,

Brammer²,

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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1,103

914

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Two important goals in stem cell research are to control the cell proliferation without differentiation and to direct the differentiation into a specific cell lineage when desired. Here, we demonstrate such paths by controlling only the nanotopography of culture substrates. Altering the dimensions of nanotubular-shaped titanium oxide surface structures independently allowed either augmented human mesenchymal stem cell (hMSC) adhesion or a specific differentiation of hMSCs into osteoblasts by using only the geometric cues, absent of osteogenic inducing media. hMSC behavior in response to defined nanotube sizes revealed a very dramatic change in hMSC behavior in a relatively narrow range of nanotube dimensions. Small (Ϸ30-nm diameter) nanotubes promoted adhesion without noticeable differentiation, whereas larger (Ϸ70-to 100-nm diameter) nanotubes elicited a dramatic stem cell elongation (Ϸ10-fold increased), which induced cytoskeletal stress and selective differentiation into osteoblast-like cells, offering a promising nanotechnology-based route for unique orthopedics-related hMSC treatments.differentiation ͉ mesenchymal ͉ nanotopography ͉ osteogenesis ͉ proliferation N anostructures are of particular interest because they have the advantageous feature of a high surface-to-volume ratio, and they elicit a higher degree of biological plasticity compared with conventional micro-or macrostructures. In the field of biomaterial development and in vivo implant technology, the nanoscale structure and morphologenic factor of the surface have played a critical role in accelerating the rate of cell proliferation and enhancing tissue acceptance with a reduced immune response (1, 2). In terms of in vitro cell biology, there has also been much attention placed on cellular responses to their structural surroundings (3). In fact, it has been observed that macro-, micro-and nano-sized topographical factors stimulate behavioral changes in both cells and tissues. Recent studies related to the effect of nanotopography on cellular behavior indicated that osteoblast adhesion and functionality was enhanced by 30% when cultured on a nanograined Al 2 O 3 and TiO 2 substrate (4-6) compared with those cultured on a micrograined surface, and nanostructures such as TiO 2 nanotubes with Ͻ100-nm spacing showed superior characteristics in bone mineral synthesis (5). However, most of the previous studies on nanostructures and cell responses have mainly used oriented, patterned, or semiordered polymer arrays (7-9) and alumina/ polymer hybrid patterned arrays (10).The material and mechanical characteristics of titanium (Ti) metal, which has a thin native oxide layer of TiO 2 , make it an ideal orthopedic material that bonds directly to the adjacent bone surface (11,12). Fabrication of the nanostructured titanium dioxide (TiO 2 ) nanotube arrays has been a primary subject of investigation lately because of the wide range of TiO 2 applications in the fields of solar cells (13-16), photocatalysis (17-19), photoelectrolysis (20), sensors (21,22), and b...

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Stem cell fate dictated solely by altered nanotube dimension

Oh¹,

Brammer²,

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

1,103

914

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show abstract

“…These materials include the traditional pharmaceutical excipients approved by the FDA such as natural polymers, a range of synthetic polymers, and phospholipids, but also more exotic systems including hydrogels and even inorganic material such as TiO 2 [41][42][43][44]. Naturally-occurring polymers remain a key focus of research interest because of their abundant supply and relatively environmentally-friendly preparation routes.…”

Section: Introductionmentioning

confidence: 99%

Nanosized sustained-release drug depots fabricated using modified tri-axial electrospinning

Yang

et al. 2017

Acta Biomaterialia

123

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Nanosized sustainedrelease drug depots fabricated using modified tri-axial electrospinning, Acta Biomaterialia (2017), doi: http:// dx.doi.org/10. 1016/j.actbio.2017.01.069 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.Nanosized sustained-release drug depots fabricated using modified tri-axial electrospinning Abstract:Nanoscale drug depots, comprising a drug reservoir surrounded by a carrier membrane, are much sought after in contemporary pharmaceutical research. Using cellulose acetate (CA) as a filament-forming polymeric matrix and ferulic acid (FA) as a model drug, nanoscale drug depots in the form of core-shell fibers were designed and fabricated using a modified tri-axial electrospinning process. This employed a solvent mixture as the outer working fluid, as a result of which a robust and continuous preparation process could be achieved. The fiber-based depots had a linear morphology, smooth surfaces, and an average diameter of 0.62 ± 0.07 µm. Electron microscopy data showed them to have clear core-shell structures, with the FA encapsulated inside a CA shell. X-ray diffraction and IR spectroscopy results verified that FA was present in the crystalline physical form. In vitro dissolution tests revealed that the fibers were able to provide close to zero-order release over 36 h, with no initial burst release and minimal tailing-off. The release properties of the depot systems were much improved over monolithic CA/FA fibers, which exhibited a significant burst release and also considerable tailing-off at the end of the release experiment. Here we thus demonstrate the concept of using modified tri-axial electrospinning to design and develop new types of heterogeneous nanoscale biomaterials.

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“…To address overloading, processing titanium through severe plastic deformation (SPD), such as high pressure torsion (HPT) [4], can increase hardness and strength whilst maintaining good ductility without introducing harmful or costly additional elements [5]. In addition, modifying the natural oxide layer of titanium, which provides remarkable surface properties, can address issues related to insufficient osseointegration [6] and undesired cell growth [7][8][9]. Titanium dioxide nanotubes (TNT) made by electrochemical anodization can modify the oxide layer, which can be utilized to positively affect cellular behaviours, such as migration [9], adhesion [10], proliferation [7,10,11] and differentiation [12,13].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, modifying the natural oxide layer of titanium, which provides remarkable surface properties, can address issues related to insufficient osseointegration [6] and undesired cell growth [7][8][9]. Titanium dioxide nanotubes (TNT) made by electrochemical anodization can modify the oxide layer, which can be utilized to positively affect cellular behaviours, such as migration [9], adhesion [10], proliferation [7,10,11] and differentiation [12,13]. By modifying the surfaces of SPD processed titanium with TNT layers, there is a possibility to simultaneously increase the bulk and surface properties, therefore further increasing the success rate of titanium implants.…”

Section: Introductionmentioning

confidence: 99%

The influence of surface roughness and high pressure torsion on the growth of anodic titania nanotubes on pure titanium

Gao

Starink

2016

Applied Surface Science

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Anodic titanium dioxide nanotube (TNT) arrays have wide applications in photocatalytic, catalysis, electronics, solar cells and biomedical implants. When TNT coatings are combined with severe plastic deformation (SPD), metal processing techniques which efficiently improve the strength of metals, a new generation of biomedical implant is made possible with both improved bulk and surface properties. This work investigated the effect of processing by high pressure torsion (HPT) and different mechanical preparations on the substrate and subsequently on the morphology of TNT layers. HPT processing was applied to refine the grain size of commercially pure titanium samples and substantially improved their strength and hardness. Subsequent anodization at 30 V in 0.25 wt. % NH 4 F for 2 hours to form TNT layers on sample surfaces prepared with different mechanical preparation methods was carried out. It appeared that the local roughness of the titanium surface on a microscopic level affected the TNT morphology more than the macroscopic surface roughness. For HPTprocessed sample, the substrate has to be pre-treated by a mechanical preparation finer than 4000 grit for HPT to have a significant influence on TNTs. During the formation of TNT layers the oxide dissolution rate was increased for the ultrafine-grained microstructure formed due to HPT processing.

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Enhanced Cellular Mobility Guided by TiO2 Nanotube Surfaces

Cited by 263 publications

References 22 publications

Stem cell fate dictated solely by altered nanotube dimension

Stem cell fate dictated solely by altered nanotube dimension

Nanosized sustained-release drug depots fabricated using modified tri-axial electrospinning

The influence of surface roughness and high pressure torsion on the growth of anodic titania nanotubes on pure titanium

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