Enhanced chondrogenesis differentiation of human induced pluripotent stem cells by MicroRNA-140 and transforming growth factor beta 3 (TGFβ3)

Mahboudi, Hossein; Soleimani, Masoud; Enderami, Seyed Ehsan; Kehtari, Mousa; Ardeshirylajimi, Abdolreza; Eftekhary, Mohamad; Kazemi, Bahram

doi:10.1016/j.biologicals.2018.01.005

Cited by 21 publications

(15 citation statements)

References 39 publications

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“…Immunofluorescence staining confirmed the presence of cells, positively stained for NANOG, but after a prolonged time of differentiation, their amount decreased. A similar observation was noticed in several iPSC cell-based protocols [24,30,40,41]. Further, we investigated how those conditions and proposed changes will influence the chondrogenic differentiation process via the analysis of the key role gene regulators of chondrogenesis such as FGFR3, SMAD3, NCAM1 , and CDH2 .…”

Section: Discussionsupporting

confidence: 68%

Chondrogenic Differentiation of Pluripotent Stem Cells under Controllable Serum-Free Conditions

Lach

Wróblewska

Kulcenty

et al. 2019

IJMS

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The repair of damaged articular cartilage using currently available implantation techniques is not sufficient for the full recovery of patients. Pluripotent stem cells (iPSC)-based therapies could bring new perspectives in the treatment of joint diseases. A number of protocols of in vitro differentiation of iPSC in chondrocytes for regenerative purposes have been recently described. However, in order to use these cells in clinics, the elimination of animal serum and feeder cells is essential. In our study, a strictly defined and controllable protocol was designed for the differentiation of pluripotent stem cells (BG01V, ND 41658*H, GPCCi001-A) in chondrocyte-like cells in serum- and a feeder cell-free system, using the embryoid bodies step. The extension of the protocol and culture conditions (monolayer versus 3D culture) was also tested after the initial 21 days of chondrogenic differentiation. Promotion of the chondrogenic differentiation in 3D culture via the elevated expression of genes related to chondrogenesis was achieved. Using immunofluorescence and immunohistochemistry staining techniques, the increased deposition of the specific extracellular matrix was indicated. As a result, chondrocyte-like cells in the early stages of their differentiation using pellet culture under fully controlled and defined conditions were obtained.

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Section: Discussionsupporting

confidence: 68%

Chondrogenic Differentiation of Pluripotent Stem Cells under Controllable Serum-Free Conditions

Lach

Wróblewska

Kulcenty

et al. 2019

IJMS

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“…These cells have several limitations that introduce induced pluripotent stem cells (iPSCs), which could be derived from epithelial cells with overexpression of the four transcription factors, which are specifically for embryonic stage (Ardeshirylajimi, ; Takahashi & Yamanaka, ). These iPSCs can be differentiated into neurons (Salimi, Nadri, Ghollasi, Khajeh, & Soleimani, ), heart muscle (Takahashi et al, ), cartilage (Mahboudi et al, ), bone (Ardeshirylajimi & Soleimani, ), hepatocytes (Mahmoodinia Maymand et al, ), and insulin producing cells (Mansour et al, ). These cells have the same differentiation potential as that of embryonic stem cells (ESCs), but without its ethical issues, and as these cells are taken from the patient themselves, the rejection percentage of the transplant is zero; also, its potential is not age‐dependent and is increased in adult stem cells (Hwang et al, ; Jung, ).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Poly (3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) improved osteogenic differentiation of the human induced pluripotent stem cells while considered as an artificial extracellular matrix

Hosseini

Soleimanifar

Aidun

et al. 2018

Journal Cellular Physiology

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Cocell polymers can be the best implants for replacing bone defects in patients. The pluripotent stem cells produced from the patient and the nanofibrous polymeric scaffold that can be completely degraded in the body and its produced monomers could be also usable are the best options for this implant. In this study, electrospun poly (3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) (PHBV) nanofibers were fabricated and characterized and then osteogenic differentiation of the human‐induced pluripotent stem cells (iPSCs) was investigated while cultured on PHBV scaffold. MTT results showed that cultured iPSCs on PHBV proliferation were increased compared to those cultured on tissue culture polystyrene (TCPS) as the control. Alkaline phosphatase (ALP) activity and calcium content were also significantly increased in iPSCs cultured on PHBV compared to the cultured on TCPS under osteogenic medium. Gene expression evaluation demonstrated that Runx2, collagen type I, ALP, osteonectin, and osteocalcin were upregulated in iPSCs cultured on PHBV scaffold in comparison with those cultured on TCPS for 2 weeks. Western blot analysis have shown that osteocalcin and osteopontin expression as two major osteogenic markers were increased in iPSCs cultured on PHBV scaffold. According to the results, nanofiber‐based PHBV has a promising potential to increase osteogenic differentiation of the stem cells and iPSCs‐PHBV as a cell‐co‐polymer construct demonstrated that has a great efficiency for use as a bone tissue engineered bioimplant.

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“… 17 MiR-140 has also been validated to enhance chondrogenesis differentiation of human iPSCs with transforming growth factor beta 3 (TGFβ3). 18 …”

Section: Discussionmentioning

confidence: 99%

MicroRNA-22 enhances the differentiation of mouse induced pluripotent stem cells into alveolar epithelial type II cells

et al. 2020

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Considerable evidence has verified that microRNAs (miRNAs) play important roles in various cellular processes including differentiation. However, the regulatory roles of miRNAs involved in the differentiation of induced pluripotent stem cells (iPSC) into lung epithelial cells are still unknown. In this study, we first evaluated the current protocols to differentiate iPSC into alveolar epithelial type II (AEC II) cells, but the efficiency is low. We next identified that miR-22 can efficiently enhance the differentiation of iPSC into AEC II cells under the stimulation of proper growth factors and growing on appropriate matrix. Moreover, the AEC II cells generated from iPSC with miR-22 overexpression can proliferate and secrete lung surfactant. Here, we discovered a previously unknown interaction between miR-22 and iPSC differentiation but also provide a potential target for the effective derivation of AEC II from iPSCs for cell-based therapy.

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Enhanced chondrogenesis differentiation of human induced pluripotent stem cells by MicroRNA-140 and transforming growth factor beta 3 (TGFβ3)

Cited by 21 publications

References 39 publications

Chondrogenic Differentiation of Pluripotent Stem Cells under Controllable Serum-Free Conditions

Chondrogenic Differentiation of Pluripotent Stem Cells under Controllable Serum-Free Conditions

Poly (3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) improved osteogenic differentiation of the human induced pluripotent stem cells while considered as an artificial extracellular matrix

MicroRNA-22 enhances the differentiation of mouse induced pluripotent stem cells into alveolar epithelial type II cells

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