Enhanced Chondrogenic Capacity of Mesenchymal Stem Cells After TNFα Pre-treatment

Voskamp, Chantal; Koevoet, Wendy; Somoza, Rodrigo A.; Caplan, Arnold I.; Lefebvre, Véronique; Osch, Gerjo J.V.M. van; Narcisi, Roberto

doi:10.3389/fbioe.2020.00658

Cited by 14 publications

(12 citation statements)

References 47 publications

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“…Nowadays, lots of studies were evaluated for the enhanced efficacy of MSC using culturing platform for therapeutic application. Exposure to TNF-α during in vitro culture, MSCs reveals an increase in migration, proliferation, and the osteogenic capacity [115]. Poly-L-lactide-co-ε-caprolactone (PLCL) electrospun fiber sheets also enhanced the paracrine signaling of MSCs for cartilage regeneration [114].…”

Section: Enhanced Msc Functionmentioning

confidence: 99%

State of the Art: The Immunomodulatory Role of MSCs for Osteoarthritis

Kwon

Kim

Jeon

et al. 2022

IJMS

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Osteoarthritis (OA) has generally been introduced as a degenerative disease; however, it has recently been understood as a low-grade chronic inflammatory process that could promote symptoms and accelerate the progression of OA. Current treatment strategies, including corticosteroid injections, have no impact on the OA disease progression. Mesenchymal stem cells (MSCs) based therapy seem to be in the spotlight as a disease-modifying treatment because this strategy provides enlarged anti-inflammatory and chondroprotective effects. Currently, bone marrow, adipose derived, synovium-derived, and Wharton’s jelly-derived MSCs are the most widely used types of MSCs in the cartilage engineering. MSCs exert immunomodulatory, immunosuppressive, antiapoptotic, and chondrogenic effects mainly by paracrine effect. Because MSCs disappear from the tissue quickly after administration, recently, MSCs-derived exosomes received the focus for the next-generation treatment strategy for OA. MSCs-derived exosomes contain a variety of miRNAs. Exosomal miRNAs have a critical role in cartilage regeneration by immunomodulatory function such as promoting chondrocyte proliferation, matrix secretion, and subsiding inflammation. In the future, a personalized exosome can be packaged with ideal miRNA and proteins for chondrogenesis by enriching techniques. In addition, the target specific exosomes could be a gamechanger for OA. However, we should consider the off-target side effects due to multiple gene targets of miRNA.

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Section: Enhanced Msc Functionmentioning

confidence: 99%

State of the Art: The Immunomodulatory Role of MSCs for Osteoarthritis

Kwon

Kim

Jeon

et al. 2022

IJMS

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“…TNF-α and IL-1β might hamper chondrogenesis of mesenchymal stromal cells, through NF-κB pathway, inhibition of SOX9 expression, and increase the synthesis of degradative enzymes involved in cartilage degeneration, like matrix metalloproteinases (MMPs) ( Jagielski et al, 2014 ). Other studies suggest that in vitro exposure to TNF-α might increase proliferation, migration, and osteogenic differentiation of MSCs ( Voskamp et al, 2020 ). In our system, BM- or WJ-3D culture showed an upregulation of all pro-inflammatory cytokine genes explored at day 8, except for IL-6 that showed a poor upregulation followed by a decrease at day 16, as also observed at the protein levels by measuring cytokines in culture medium.…”

Section: Discussionmentioning

confidence: 99%

3D in-vitro cultures of human bone marrow and Wharton’s jelly derived mesenchymal stromal cells show high chondrogenic potential

Lamparelli

Ciardulli

Giudice

et al. 2022

Front. Bioeng. Biotechnol.

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In this study, chondrogenic potentials of 3D high-density cultures of Bone Marrow (BM) and Wharton’s Jelly (WJ)-derived mesenchymal stromal cells (MSCs) was investigated by chondrogenesis- and cytokine-related gene expression over a 16-day culture period supplemented with human transforming growth factor (hTGF)-β1 at 10 ng/ml. In BM-MSC 3D models, a marked upregulation of chondrogenesis-related genes, such as SOX9, COL2A1, and ACAN (all p < 0.05) and formation of spherical pellets with structured type II collagen fibers were observed. Similarly, WJ-based high-density culture appeared higher in size and more regular in shape, with a significant overexpression of COL2A1 and ACAN (all p < 0.05) at day 16. Moreover, a similar upregulation trend was documented for IL-6 and IL-10 expression in both BM and WJ 3D systems. In conclusion, MSC-based high-density cultures can be considered a promising in vitro model of cartilage regeneration and tissue engineering. Moreover, our data support the use of WJ-MSCs as a valid alternative for chondrogenic commitment of stem cells in regenerative medicine.

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“…31 Tumour necrosis factor α (TNF-α) exposure during MSCs expansion could modulate SOX11 levels and WNT/β-catenin signalling, increasing the chondrogenic differentiation capacity. 100 Besides adding growth factors directly, chondrogenic genes have been transfected into MSCs to increase growth factor secretion and promote chondrogenic differentiation. Liu et al 101 testified that ADSCs with BMP-14 gene transfer by adenovirus could enhance chondrogenic differentiation of ADSCs and accelerate cartilage defect repair in rabbits.…”

Section: Human Articular Ccsmentioning

confidence: 99%

“…PDGF‐BB could promote the migration of BMSCs in fibrin‐HA and HA‐Tyramine hydrogels with different crosslinking densities in vitro 31 . Tumour necrosis factor α (TNF‐α) exposure during MSCs expansion could modulate SOX11 levels and WNT/β‐catenin signalling, increasing the chondrogenic differentiation capacity 100 …”

Section: Chondrogenic Culture Of Adscsmentioning

confidence: 99%

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Cartilage 3D bioprinting for rhinoplasty using adipose‐derived stem cells as seed cells: Review and recent advances

et al. 2023

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Nasal deformities due to various causes affect the aesthetics and use of the nose, in which case rhinoplasty is necessary. However, the lack of cartilage for grafting has been a major problem and tissue engineering seems to be a promising solution. 3D bioprinting has become one of the most advanced tissue engineering methods. To construct ideal cartilage, bio-ink, seed cells, growth factors and other methods to promote chondrogenesis should be considered and weighed carefully. With continuous progress in the field, bio-ink choices are becoming increasingly abundant, from a single hydrogel to a combination of hydrogels with various characteristics, and more 3D bioprinting methods are also emerging. Adipose-derived stem cells (ADSCs) have become one of the most popular seed cells in cartilage 3D bioprinting, owing to their abundance, excellent proliferative potential, minimal morbidity during harvest and lack of ethical considerations limitations. In addition, the coculture of ADSCs and chondrocytes is commonly used to achieve better chondrogenesis. To promote chondrogenic differentiation of ADSCs and construct ideal highly bionic tissue-engineered cartilage, researchers have used a variety of methods, including adding appropriate growth factors, applying biomechanical stimuli and reducing oxygen tension. According to the process and sequence of cartilage 3D bioprinting, this review summarizes and discusses the selection of hydrogel and seed cells (centered on ADSCs), the design of printing, and methods for inducing the chondrogenesis of ADSCs. | INTRODUCTIONTrauma, burn, tumour, surgery, or congenital malformation of nasal cartilage may compromise a nasal deformity or lead to nasal airway dysfunction, affecting the aesthetics and utility of the nose. 1 Rhinoplasty usually requires trimming of nasal cartilage and implantation of grafts. Therefore, finding the most suitable and compatible cartilage graft material is essential. Ideally, the engineered cartilage graft should have properties to meet the clinical needs of the recipient site, including being able to withstand the external forces in the nasal reconstructive environment, biocompatible, and biologically active to support and promote tissue integration and healing. Besides, it should be readily available or easy to access without contributing to donor site morbidity. Moreover, from a surgical point of view, the graft material needs to be big enough in the case of intraoperative trimming to meet the needs of patients.Chong Zhang and Guanhuier Wang contributed equally to this work.

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Enhanced Chondrogenic Capacity of Mesenchymal Stem Cells After TNFα Pre-treatment

Cited by 14 publications

References 47 publications

State of the Art: The Immunomodulatory Role of MSCs for Osteoarthritis

State of the Art: The Immunomodulatory Role of MSCs for Osteoarthritis

3D in-vitro cultures of human bone marrow and Wharton’s jelly derived mesenchymal stromal cells show high chondrogenic potential

Cartilage 3D bioprinting for rhinoplasty using adipose‐derived stem cells as seed cells: Review and recent advances

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