Enhanced cleanup efficiency hydroxy functionalized-magnetic graphene oxide and its comparison with magnetic carboxyl-graphene for PRiME pass-through cleanup of strychnine and brucine in human plasma samples

Ma, Jianbo; Qiu, Hai-Wen; Rui, Qiu-Hong; Liao, Yanling; Chen, Yanmin; Xu, Jin; Zhang, Yun; Zhu, Yan; Zhao, Yanfang

doi:10.1016/j.aca.2018.03.008

Cited by 19 publications

(6 citation statements)

References 27 publications

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“…Brucine is one of the major bioactive and toxic constituents of Strychnos nux-vomica L., which is a traditional Chinese medicine, and widely used for the treatment of rheumatic pain, cancers and so on (Savalia et al 2017; Ma et al 2018). While, clinical applications of brucine are limited due to its severe adverse drug effects, especially neurotoxicity (Li et al 2018; ).…”

Section: Introductionmentioning

confidence: 99%

Brucine-Induced Neurotoxicity by Targeting Caspase 3: Involvement of PPARγ/NF-κB/Apoptosis Signaling Pathway

et al. 2022

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Brucine, a weak alkaline indole alkaloid, is one of the main bioactive and toxic constituents of Strychnos nux-vomica L., which exerts multiple pharmacological activities, such as anti-tumor, anti-in ammatory, analgesic effect. However, its potential toxic effects limited its clinical application, especially central nervous system toxicity. The present study was designed to investigate the neurotoxicity and mechanism of brucine. Our results showed that brucine signi cantly induced Neuro-2a cells and primary astrocytes death, as evidenced by MTT assay and LDH release. Moreover, transcriptome analysis indicated that PPAR/NF-κB and apoptosis signaling pathways were involved in the brucine-induced cytotoxicity in Neuro-2a cells. Subsequently, in fact, brucine evidently inhibited PPARγ, promoted phosphorylation of NF-κB. Furthermore, PPARγ inhibitor aggravated the neurotoxicity, while, NF-κB inhibitor substantially reversed brucine-induced neurotoxicity. Moreover, brucine also signi cantly induced neuronal apoptosis and triggered increase in ratio of Bax/Bcl-2, level of cleaved caspase-3, as well as its activity as evidenced by TUNEL staining and Western blot. Furthermore, molecular docking analysis predicted that brucine directly bound to caspase 3. Intriguingly, a caspase 3 inhibitor (Z-DEVE-FMK) largely abolished the neurotoxicity of brucine. Our results reveal that brucine-induced neurotoxicity via activation of PPARγ/NF-κB/caspase 3-dependent apoptosis pathway. These ndings will provide a novel strategy against brucine-induced neurotoxicity.

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Section: Introductionmentioning

confidence: 99%

Brucine-Induced Neurotoxicity by Targeting Caspase 3: Involvement of PPARγ/NF-κB/Apoptosis Signaling Pathway

et al. 2022

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“…In 2018, an enhanced cleanup efficiency hydroxy functionalized-magnetic GO (EH-MAG-GO) modified by hydroxyl group was successfully synthesized by Ma and co-workers ( Figure 16 and Figure 17 ) [ 9 ]. EH-MAG-GO was used as an adsorbent in the PRiME pass-through cleanup procedure for the cleanup of strychnine and brucine prior to LC-MS/MS analysis.…”

Section: Synthesis Of Nanomaterials and Their Applications In Sample ...mentioning

confidence: 99%

“…At present, solid phase extraction (SPE), with higher adsorption rate and better reproducibility, is widely used in the preconcentration of low concentration substances and the purification of complex samples [ 4 ]. As well as the SPE methods, various efficient and environmentally friendly pretreatment methods based on SPE, including solid phase microextraction (SPME), dispersed solid phase extraction (dSPE), magnetic solid-phase extraction (MSPE), the QuEChERS (quick, easy, cheap, effective, rugged, and safe) method [ 5 , 6 , 7 , 8 ], and the PRiME (process, robustness, improvements, matrix effects, and ease of use) pass-through cleanup procedure [ 9 , 10 , 11 , 12 ], have been developed. In these sample pretreatment methods, adsorbent with uniform particle size distribution, good dispersion, and high-efficiency adsorption capacity is the key factor for the performance of sample pretreatment.…”

Section: Introductionmentioning

confidence: 99%

Nanomaterials with Excellent Adsorption Characteristics for Sample Pretreatment: A Review

Liu

et al. 2022

Nanomaterials

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Sample pretreatment in analytical chemistry is critical, and the selection of materials for sample pretreatment is a key factor for high enrichment ability, good practicality, and satisfactory recoveries. In this review, the recent progress of the sample pretreatment methods based on various nanomaterials (i.e., carbon nanomaterials, porous nanomaterials, and magnetic nanomaterials) with excellent adsorption efficiency, selectivity, and reproducibility, as well as their applications, are presented. Due to the unique nanoscale physical–chemical properties, magnetic nanomaterials have been used for the extraction of target analytes by easy-to-handle magnetic separation under a magnetic field, which can avoid cumbersome centrifugation and filtration steps. This review also highlights the preparation process and reaction mechanism of nanomaterials used in the sample pretreatment methods, which have been applied for the extraction organophosphorus pesticides, fluoroquinolone antibiotics, phenoxy carboxylic acids, tetracycline antibiotics, hazardous metal ions, and rosmarinic acid. In addition, the remaining challenges and future directions for nanomaterials used as sorbents in the sample pretreatment are discussed.

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“…12 Furthermore, based on the PRiME passthrough cleanup method, our group has developed various carbon nanosorbents, i.e., magnetic modified carboxylgraphene and carboxyl-carbon nanotubes, and hydroxy functionalized-magnetic graphene oxide for the cleanup endogenous matrices in human plasma samples. [13][14][15][16][17] Furthermore, another TiO 2 modified covalent organic framework was prepared and used as a sorbent in the on-line pass-through cleanup method prior to the determination of anesthetic drugs in human plasma. 18 Although these carbon nanosorbents mentioned above have been successfully applied to the cleanup of blood and plasma samples and the proposed analytical method has excellent selectivity and sensitivity, the main drawback of these carbon nanosorbents is the low productivity, which greatly restricts their application in practical work.…”

Section: Introductionmentioning

confidence: 99%

Nano-titania modified cupric-based metal–organic frameworks for the on-line pass-through cleanup of phenothiazine drugs and metabolites in human plasma

Gan,

Zhao,

et al. 2022

New J. Chem.

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Enhanced cleanup efficiency hydroxy functionalized-magnetic graphene oxide and its comparison with magnetic carboxyl-graphene for PRiME pass-through cleanup of strychnine and brucine in human plasma samples

Cited by 19 publications

References 27 publications

Brucine-Induced Neurotoxicity by Targeting Caspase 3: Involvement of PPARγ/NF-κB/Apoptosis Signaling Pathway

Brucine-Induced Neurotoxicity by Targeting Caspase 3: Involvement of PPARγ/NF-κB/Apoptosis Signaling Pathway

Nanomaterials with Excellent Adsorption Characteristics for Sample Pretreatment: A Review

Nano-titania modified cupric-based metal–organic frameworks for the on-line pass-through cleanup of phenothiazine drugs and metabolites in human plasma

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