Enhanced co-production of hydrogen and poly-(R)-3-hydroxybutyrate by recombinant PHB producing E. coli over-expressing hydrogenase 3 and acetyl-CoA synthetase

Wang, Ruiyan; Shi, Zhenyu; Chen, Jinchun; Wu, Qiong; Chen, Guoqiang

doi:10.1016/j.ymben.2012.07.003

Cited by 38 publications

(19 citation statements)

References 52 publications

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“…It was reported that the fdnG and focA mutants as hosts delivered a negative factor where no PHA was observed in most of the strains. A similar situation was observed in the other study presenting the deletion of fdnG that gave an opposite effect in PHA production (Wang et al, 2012). It was believed that the deletion of fdnG and focA influenced the nitrate respiration in E. coli metabolism, thus did not contribute to the PHA metabolism (Wang et al, 2012).…”

Section: Resultssupporting

confidence: 74%

Triple knockout of frdC gltA and pta genes enhanced pHA production in Escherichia coli

Biran

Yusoff

Maeda

et al. 2018

APJMBB

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Polyhydroxyalkanoate (PHA) is a linear polyester produced through the fermentation of sugar or lipid. Biosynthesis of PHA comprises three enzymes known as acetyl-CoA acetyltransferase (phaA), acetoacetyl-CoA reductase (phaB) and PHA synthase (phaC). Comamonas sp. is one of the strains commonly used for PHA production. In order to develop higher PHA production from bacterial respond strategy, PHA biosynthesis operon of Comamonas sp. EB172 was introduced into Escherichia coli BW25113 through a pGEM-T vector. E. coli was chosen due to the complete genome information available and the absence of depolymerisation gene, phaZ. In this study, the deletion of several single genes, which are frdC, gltA, and pta, was found to be associated with PHA metabolism activity in E. coli BW25113. P1 transduction was performed to construct multiple genes knockout. The engineered strain, E. coli BW25113 frdCgltApta::kan/pGEM'-phaCAB Co , yielded the highest PHA production at 64 wt.% with 1.4 fold higher than that of control strain of E. coli BW25113/pGEM'-phaCAB Co . This strain is potential for industrial application for higher PHA production from E. coli.

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Section: Resultssupporting

confidence: 74%

Triple knockout of frdC gltA and pta genes enhanced pHA production in Escherichia coli

Biran

Yusoff

Maeda

et al. 2018

APJMBB

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show abstract

“…Wang et al 30 enhanced coproduction of hydrogen and PHB by recombinant E. coli by over expressing hydrogenase 3 and acetyl-CoA synthetase. Wang et al 30 enhanced coproduction of hydrogen and PHB by recombinant E. coli by over expressing hydrogenase 3 and acetyl-CoA synthetase.…”

Section: Phb Production Using Recombinant Bacteriamentioning

confidence: 99%

Microbial Poly-3-Hydroxybutyrate and Related Copolymers

Sindhu

Pandey

2015

Industrial Biorefineries &Amp; White Biotechnology

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“…The overexpression of Zwf1p triggered the production of xylitol, GDP‐L‐fucose, e‐caprolactone by enhanced NADPH levels [12, 25, 26]. The Acs1p overexpression influenced the production of many biochemicals such as hydrogen, isopropanol, butanol by increasing the carbon flux into a desired product and decreasing acetate accumulation, which was toxic to cell growth [27, 28]. When cellobiose and xylose were consumed simultaneously, overexpression of S. cerevisiae Idp2p (isocitrate dehydrogenase) increased the NADPH availability and allowed a 63% improvement in xylitol productivity [29].…”

Section: Discussionmentioning

confidence: 99%

Dual utilization of NADPH and NADH cofactors enhances xylitol production in engineered Saccharomyces cerevisiae

Lee

et al. 2015

Biotechnology Journal

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Xylitol, a natural sweetener, can be produced by hydrogenation of xylose in hemicelluloses. In microbial processes, utilization of only NADPH cofactor limited commercialization of xylitol biosynthesis. To overcome this drawback, Saccharomyces cerevisiae D452-2 was engineered to express two types of xylose reductase (XR) with either NADPH-dependence or NADH-preference. Engineered S. cerevisiae DWM expressing both the XRs exhibited higher xylitol productivity than the yeast strain expressing NADPH-dependent XR only (DWW) in both batch and glucose-limited fed-batch cultures. Furthermore, the coexpression of S. cerevisiae ZWF1 and ACS1 genes in the DWM strain increased intracellular concentrations of NADPH and NADH and improved maximum xylitol productivity by 17%, relative to that for the DWM strain. Finally, the optimized fed-batch fermentation of S. cerevisiae DWM-ZWF1-ACS1 resulted in 196.2 g/L xylitol concentration, 4.27 g/L h productivity and almost the theoretical yield. Expression of the two types of XR utilizing both NADPH and NADH is a promising strategy to meet the industrial demands for microbial xylitol production.

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Enhanced co-production of hydrogen and poly-(R)-3-hydroxybutyrate by recombinant PHB producing E. coli over-expressing hydrogenase 3 and acetyl-CoA synthetase

Cited by 38 publications

References 52 publications

Triple knockout of frdC gltA and pta genes enhanced pHA production in Escherichia coli

Triple knockout of frdC gltA and pta genes enhanced pHA production in Escherichia coli

Microbial Poly-3-Hydroxybutyrate and Related Copolymers

Dual utilization of NADPH and NADH cofactors enhances xylitol production in engineered Saccharomyces cerevisiae

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