Enhanced CO2 fixation and lipid production of Chlorella vulgaris through the carbonic anhydrase complex

You, Sung Hyun; Ko, Young Jin; Shin, Seung-Soo; Hwang, Dong hyeuk; Kang, Dae Hee; Park, Hyeon Min; Han, Sung Ok

doi:10.1016/j.biortech.2020.124072

Cited by 29 publications

(12 citation statements)

References 46 publications

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“…Recently, thermostable CA was immobilized on the surface of Chlorella sorokiniana, or CA was complexed for immobilization on the surface of C. vulgaris . However, this study is the first report in which CA was expressed in the cytosol and CO 2 was successfully fixed into fatty acids.…”

Section: Results and Discussionmentioning

confidence: 89%

“…In addition, bicarbonate is a cosubstrate used to produce malonyl-CoA from acetyl-CoA at the initiation of fatty acid biosynthesis. Previous studies of photosynthetic green algae have shown increased lipid production upon the addition of bicarbonate. ,, Therefore, the effect of bicarbonate addition on Y. lipolytica was analyzed in this study.…”

Section: Results and Discussionmentioning

confidence: 99%

“…In metabolic engineering studies in which microorganisms utilize CO 2 to balance NADH and produce useful products, CO 2 was successfully immobilized, but its amount was small and unstable . In the field of CO 2 utilization research, for decades, studies have been conducted to fix CO 2 in an enzymatic manner using CA. ,, Research studies have mainly focused on increasing enzymatic activity or thermal stability. In order to increase the CA activity, CA was immobilized on the surface of E.…”

Section: Results and Discussionmentioning

confidence: 99%

“…The samples were treated with Folch’s reagent and derivatized in the same manner as in previous studies. Samples were analyzed by an Agilent GC 7890 with an FID and HP-5MS column (30 m, 0.25 mm i.d., 0.25 μm film) . The GC analysis conditions are as follows: the starting temperature was maintained at 100 °C for 5 min, the temperature of the oven increased 15 °C per min for 10 min to reach 250 °C, and the temperature was maintained at 250 °C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…Carbonic anhydrase (CA), an enzyme that converts CO 2 to bicarbonate, was complexed and immobilized on the surface of C. vulgaris to increase growth and lipid production . However, the production of fatty acids or value-added fatty acid-derived biochemicals has not been reported in non-photosynthetic microorganisms with CO 2 utilization to the best of our knowledge.…”

Section: Introductionmentioning

confidence: 99%

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Non-Photosynthetic CO₂ Utilization to Increase Fatty Acid Production in Yarrowia lipolytica

You

Park

Lee

et al. 2021

J. Agric. Food Chem.

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Metabolic engineering of non-photosynthetic microorganisms to increase the utilization of CO2 has been focused on as a green strategy to convert CO2 into valuable products such as fatty acids. In this study, a CO2 utilization pathway involving carbonic anhydrase and biotin carboxylase was formed to recycle CO2 in the oleaginous yeast Yarrowia lipolytica, thereby increasing the production of fatty acids. In the recombinant strain in which the CO2 utilization pathway was introduced, the production of fatty acids was 10.7 g/L, which was 1.5-fold higher than that of the wild-type strain. The resulting strain had a 1.4-fold increase in dry cell mass compared to the wild-type strain. In addition, linoleic acid was 47.7% in the fatty acid composition of the final strain, which was increased by 11.6% compared to the wild-type strain. These results can be applied as an essential technology for developing efficient and eco-friendly processes by directly utilizing CO2.

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Section: Results and Discussionmentioning

confidence: 89%

Section: Results and Discussionmentioning

confidence: 99%

Section: Results and Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Non-Photosynthetic CO₂ Utilization to Increase Fatty Acid Production in Yarrowia lipolytica

You

Park

Lee

et al. 2021

J. Agric. Food Chem.

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Photosynthesis Characterization of Mutant Algae and Enhanced Carbon Fixation of Algae–Bacteria Symbiosis Treating Municipal Wastewater

Zhao,

Liu,

Wang

et al. 2024

Fuel Cells

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Algae–bacteria symbiosis (ABS) as a sustainable wastewater treatment process has drawn mounting attention. However, nontrivial CO2 emissions were still present in municipal wastewater treatment due to the inadequate carbon fixation efficiency of microalgae under low carbon level. The obtained UV‐induced mutant Chlorella vulgaris MIHL4 performed higher carbon fixation capability (14.5%) and biomass productivity (25.3%) with improved photosynthetic fluorescence parameters and enzyme activities compared to wild‐type C. vulgaris. Transcriptome analyses showed pathways related to the carbon fixation and carbon catabolism were significantly up‐regulated in MIHL4. Compared with ABS inoculated with wild‐type C. vulgaris, CO2 emissions were significantly reduced by 32.1%–38.3% in ABS inoculated with MIHL4, where the biomass growth, metabolic activity, and sludge granulation were enhanced. Chlorella responsible for carbon fixation was the dominant population (19.3%) in ABS inoculated with MIHL4, in which the abundance of functional microbes and genes associated with photosynthesis as well as nutrient removal increased.

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Embracing Industry 4.0 Ingredients in Synthetic Biology for the Development of Carbon–Neutral Economy

Gurawalia,

Shinde,

Sharma

2024

Green Energy and Technology

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Enhanced CO2 fixation and lipid production of Chlorella vulgaris through the carbonic anhydrase complex

Cited by 29 publications

References 46 publications

Non-Photosynthetic CO₂ Utilization to Increase Fatty Acid Production in Yarrowia lipolytica

Non-Photosynthetic CO₂ Utilization to Increase Fatty Acid Production in Yarrowia lipolytica

Photosynthesis Characterization of Mutant Algae and Enhanced Carbon Fixation of Algae–Bacteria Symbiosis Treating Municipal Wastewater

Embracing Industry 4.0 Ingredients in Synthetic Biology for the Development of Carbon–Neutral Economy

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Enhanced CO2 fixation and lipid production of Chlorella vulgaris through the carbonic anhydrase complex

Cited by 29 publications

References 46 publications

Non-Photosynthetic CO2 Utilization to Increase Fatty Acid Production in Yarrowia lipolytica

Non-Photosynthetic CO2 Utilization to Increase Fatty Acid Production in Yarrowia lipolytica

Photosynthesis Characterization of Mutant Algae and Enhanced Carbon Fixation of Algae–Bacteria Symbiosis Treating Municipal Wastewater

Embracing Industry 4.0 Ingredients in Synthetic Biology for the Development of Carbon–Neutral Economy

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Product

Resources

About

Non-Photosynthetic CO₂ Utilization to Increase Fatty Acid Production in Yarrowia lipolytica

Non-Photosynthetic CO₂ Utilization to Increase Fatty Acid Production in Yarrowia lipolytica