Enhanced CXCL1 production and angiogenesis in adenosine‐mediated lung disease

Mohsenin, Amir; Burdick, Marie D.; Molina, Jose G.; Keane, Michael P.; Blackburn, Michael R.

doi:10.1096/fj.06-7301com

Cited by 32 publications

(27 citation statements)

References 57 publications

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“…These include cytokines CXCL1, CXCL6, and CXCL8, and VEGFA that can all function as growth and angiogenic factors (38,39). Furthermore, increased expression of CXCL1, CXCL8, and VEGFA correlated significantly with severe disease ( Table 4), suggesting that angiogenesis, a histological hallmark of RRP, is central to the pathology of this disease.…”

Section: Discussionmentioning

confidence: 99%

Immune Dysregulation and Tumor-Associated Gene Changes in Recurrent Respiratory Papillomatosis: A Paired Microarray Analysis

DeVoti

Rosenthal

et al. 2008

Mol Med

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Recurrent respiratory papillomas (RRP) are benign airway tumors, caused primarily by human papillomaviruses (HPV) types 6 and 11. The disease is characterized by multiple recurrences after surgical removal, with limited effective therapy. To identify novel targets for future therapy, we established transcriptional profiles for actively growing papillomas compared with autologous, clinically normal, laryngeal epithelia (adjacent tissue). Total ribonucleic acid (RNA) from 12 papillomas and 12 adjacent tissues were analyzed by microarray, and the matched sets of tissues compared by paired t test, to identify differentially expressed genes in papilloma tissues while minimizing variations intrinsic to individual patients. Quantitative polymerase chain reaction (PCR) was used to confirm the relative expression levels for a subset of genes. Within the 109 differentially expressed transcripts whose expression varied at least three-fold were two large groups of genes with related functions. The first group consisted of 18 genes related to host defense, including both innate and adaptive immunity. The second group contained 37 genes that likely contribute to growth of papillomas as benign tumors, since the altered pattern of expression also had been reported previously in many cancers. Our results support our previous studies that document a systemic T H 2-like adaptive immune response in RRP, and suggest that there is a role for altered innate immunity in RRP as well. We propose that HPV 6 and 11 infection establishes a tumorigenic microenvironment characterized by alteration of both innate inflammatory signals and adaptive immune responses that prevent effective T H 1-like response, in conjunction with altered expression of numerous genes that regulate cellular growth and differentiation.

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Section: Discussionmentioning

confidence: 99%

Immune Dysregulation and Tumor-Associated Gene Changes in Recurrent Respiratory Papillomatosis: A Paired Microarray Analysis

DeVoti

Rosenthal

et al. 2008

Mol Med

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“…(Table 1). All primers were designed and tested in house, except for Cxcl-1 [29]. Relative amounts of mRNA were determined using the Pfaffl-method [30] with HPRT and GAPDH as reference genes.…”

Section: Single Cell Suspensions Musclementioning

confidence: 99%

Dynamics of APC recruitment at the site of injection following injection of vaccine adjuvants

Aalst

Ludwig

Kooten

et al. 2017

Vaccine

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a b s t r a c tVaccines often contain adjuvants to strengthen the response to the vaccine antigen. However, their modes of action at the site of injection (SOI) are poorly understood. Therefore, we assessed the local effects of adjuvant on the innate immune system in mice. We investigated the safe, widely used adjuvants MF59 and aluminum hydroxide (alum), as well as trehalose-6,60 -dibehenate (TDB), Complete Freund's Adjuvant (CFA) and the Toll-Like-Receptor-ligands lipopolysaccharide (LPS) and Pam3CysSerLys4 (Pam 3 CSK 4 ). We assessed muscle immune cell infiltration after adjuvant injection and observed 16 h post immunization (hpi) an increased influx with CFA, MF59 and TDB, but not with alum, LPS or Pam 3 CSK 4 . An elevated influx with the latter three became visible only 72 hpi. Contribution of granulocytes, macrophages and dendritic cells to the influx differed per adjuvant and in time. Adjuvants generally induced a local pro-inflammatory micro-milieu that was transient except for CFA and TDB. The gene expression of CXCL-1, CCL-2 and CCL-5, involved in recruitment of immune cells, varied per adjuvant and corresponded grossly with the observed influx of granulocytes and monocytes/ macrophages. Muscles injected with CFA or MF59 (when co-injected with peptide) resulted in APC ex vivo capable to induce proliferation of peptide-specific T-cells. By adding in vitro an excess of peptide to the APC/T cell co-cultures, we observed an adjuvant-enhanced co-stimulation or antigen presentation by APC after CFA-but not MF59-injection. After TDB-injection this effect was observed only at 72 hpi, but not 24 hpi. Thus the cellular influx profile and the local cytokine and chemokine micro-milieu in the muscle were strongly influenced by the type of adjuvant. Additionally, the capacity of muscle APC to load and present antigen was affected by the adjuvant. These findings may assist the development of novel adjuvanted vaccines in a more rational manner.

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“…The overexpression of this miRNA significantly suppressed the expression of CXCL1 (a member of the CXC chemokine family) [63] . CXCL1, which is involved in EC biogenesis and angiogenesis, is known to promote neovascularization by binding G-protein-coupled receptors [64,65] .…”

Section: Endothelial Cell Differentiationmentioning

confidence: 99%

MicroRNAs as novel regulators of stem cell fate

Choi

Hwang³

2013

WJSC

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Mounting evidence in stem cell biology has shown that microRNAs (miRNAs) play a crucial role in cell fate specification, including stem cell self-renewal, lineagespecific differentiation, and somatic cell reprogramming. These functions are tightly regulated by specific gene expression patterns that involve miRNAs and transcription factors. To maintain stem cell pluripotency, specific miRNAs suppress transcription factors that promote differentiation, whereas to initiate differentiation, lineagespecific miRNAs are upregulated via the inhibition of transcription factors that promote self-renewal. Small molecules can be used in a similar manner as natural miRNAs, and a number of natural and synthetic small molecules have been isolated and developed to regulate stem cell fate. Using miRNAs as novel regulators of stem cell fate will provide insight into stem cell biology and aid in understanding the molecular mechanisms and crosstalk between miRNAs and stem cells.Ultimately, advances in the regulation of stem cell fate will contribute to the development of effective medical therapies for tissue repair and regeneration. This review summarizes the current insights into stem cell fate determination by miRNAs with a focus on stem cell self-renewal, differentiation, and reprogramming. Small molecules that control stem cell fate are also highlighted.© 2013 Baishideng. All rights reserved. Key words:MicroRNA; Stem cell fate; Differentiation; Self-renewal; Reprogramming; Small molecule Core tip: Stem cells are important in regenerative medicine applications due to their capacity to self-renew and differentiate into specific cell types. MicroRNAs (miRNAs) are short non-coding RNAs that negatively regulate gene expression at the post-transcriptional level. Recent studies suggest that miRNAs are key molecules in the regulation of stem cell fate decisions; this regulation is manifested as the fine tuning of cell-and tissue-specific gene expression. This review summarizes the current insights into stem cell fate determination by miRNAs and focuses on stem cell self-renewal, differentiation, and reprogramming. Small molecules that control stem cell fate are also highlighted.

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Enhanced CXCL1 production and angiogenesis in adenosine‐mediated lung disease

Cited by 32 publications

References 57 publications

Immune Dysregulation and Tumor-Associated Gene Changes in Recurrent Respiratory Papillomatosis: A Paired Microarray Analysis

Immune Dysregulation and Tumor-Associated Gene Changes in Recurrent Respiratory Papillomatosis: A Paired Microarray Analysis

Dynamics of APC recruitment at the site of injection following injection of vaccine adjuvants

MicroRNAs as novel regulators of stem cell fate

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