Enhanced Cytotoxic Activity of Macrophages and Suppressed Tumor Metastases in Mice Irradiated with Low Doses of X- rays

Nowosielska, Ewa M.; Wrembel-Wargocka, Jolanta; Cheda, Aneta; Lisiak, Emil; Janiak, Marek K.

doi:10.1269/jrr.0572

Cited by 49 publications

(39 citation statements)

References 24 publications

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“…injected with 1 ml of 10% Sephadex G-25 (Pharmacia, Uppsala, Sweden) and peritoneal exudate cells were collected on the selected days after cessation of the irradiations (in each case the cells were collected and pooled from at least five mice), resuspended in CM and incubated on glass Petri dishes for 2 h in SC. The glass-adherent cells with morphological features of a typical macrophage were then harvested and resuspended in CM (Nowosielska et al 2006b). …”

Section: Preparation Of Peritoneal Macrophagesmentioning

confidence: 99%

Effect of Low Doses of Low-Let Radiation on the Innate Anti-Tumor Reactions in Radioresistant and Radiosensitive Mice

Nowosielska¹,

Cheda²,

Wrembel-Wargocka³

et al. 2012

Dose-Response

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ᮀ BALB/c and C57BL/6 mice differ in their Th1/Th2 lymphocyte and M1/M2 macrophage phenotypes, radiosensitivity, and post-irradiation tumor incidence. In this study we evaluated the effects of repeated low-level exposures to X-rays on the development of artificial tumor colonies in the lungs of animals from the two strains and cytotoxic activities of natural killer (NK) cells and macrophages obtained from these mice. After ten daily irradiations of BALB/c or C57BL/6 mice with 0.01, 0.02, and 0.1 Gy X-rays NK cell-enriched splenocytes collected from the animals demonstrated significant and comparable up-regulation of their anti-tumor cytotoxic function. Likewise, peritoneal macrophages collected from the two irradiated strains of mice exhibited the similarly stimulated cytotoxicities against susceptible tumor cells and produced significantly more nitric oxide. These results were accompanied by the significantly reduced numbers of the neoplastic colonies induced in the lungs by intravenous injection of syngeneic tumor cells. The obtained results indicate that ten low-level irradiations with X-rays stimulate the generally similar anti-tumor reactions in BALB/c and C57BL/6 mice.

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Section: Preparation Of Peritoneal Macrophagesmentioning

confidence: 99%

Effect of Low Doses of Low-Let Radiation on the Innate Anti-Tumor Reactions in Radioresistant and Radiosensitive Mice

Nowosielska¹,

Cheda²,

Wrembel-Wargocka³

et al. 2012

Dose-Response

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“…injection of mice with carrageenan (CGN -a lysosomedisrupting and phagocyte-damaging compound) (Frank et al 2003) 24 hours before single WBI resulted in the almost total abrogation of the synthesis of NO in the collected peritoneal Mφ regardless of whether the cells were obtained from the irradiated or sham-exposed mice , Nowosielska et al 2006b, 2008). This finding supports a possible involvement of NO in the Mφ-mediated anti-tumor effect of the low-level exposures to X-rays.…”

Section: E M Nowosielska and Othersmentioning

confidence: 99%

“…However, peritoneal Mφ collected from the CGN-treated animals still exhibited cytotoxic activity in vitro, even in the absence of IFN-γ and LPS in the incubation medium. This observation can be explained by triggering of the synthesis of NO in the effector peritoneal Mφ by the target cells (Nowosielska et al 2006b) and/or involvement of other stimulatory mechanisms in the Mφ-mediated function. Indeed, suppression of the activity of the inducible nitric oxide synthase (iNOS) by aminoguanidine (AG -a classical inhibitor of iNOS) markedly reduced the cytolytic function of the effector peritoneal Mφ obtained from both the sham-exposed and irradiated mice (Table 4) (Nowosielska et al 2006b).…”

Section: E M Nowosielska and Othersmentioning

confidence: 99%

Immunological Mechanism of the Low-Dose Radiation-Induced Suppression of Cancer Metastases in a Mouse Model

Nowosielska¹,

Cheda²,

Wrembel-Wargocka³

et al. 2009

Dose-Response

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ᮀ According to the doctrine underlying the current radiation protection regulations each, no matter how small, exposure to ionizing radiation may be carcinogenic. However, numerous epidemiological observations demonstrate that cancer incidence and/or mortality are not elevated among inhabitants of the high-versus low-natural-background radiation areas and homes. Results of our own and other authors' studies described in this paper bear testimony to the possibility that stimulation of the anti-neoplastic immune surveillance mediated by NK lymphocytes and activated macrophages explains, at least partially, the accumulating epidemiological and experimental evidence indicating that lowlevel exposures to the low-linear energy transfer (LET) radiation inhibit the development of spontaneous and artificial metastases in humans and laboratory animals, respectively. The results presented also suggest the possibility of using low-level X-and gamma-ray exposures to cure cancer and to prevent cancer metastases. For a broader perspective, the results presented may help towards relaxing the current radiation protection regulations, especially as they apply to diagnostic and therapeutic exposures of patients to the indicated forms of radiation.

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“…It is known that an immune system plays an important role in antitumor effects of the low dose ionizing radiation. Several studies demonstrated a reduced lung metastasis in animals irradiated with the low dose radiation (<1 Gy) [7][8][9]. This effect can be associated with an activation of natural killer cells and macrophages, as well as an increased production of cytokines that play an important role in anti-metastatic effects of the ionizing radiation.…”

Section: Introductionmentioning

confidence: 99%

Macrophage and tumor cell responses to repetitive pulsed X-ray radiation

Buldakov¹,

Tretyakova²,

Ryabov

et al. 2017

J. Phys.: Conf. Ser.

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On the question of use of dosimetric devices in the registration of pulsed X-ray radiation L V Vladimirov, B V Artemiev, S E Okhrimenko et al. Abstract. To study a response of tumor cells and macrophages to the repetitive pulsed lowdose X-ray radiation. Methods. Tumor growth and lung metastasis of mice with an injected Lewis lung carcinoma were analysed, using C57Bl6. Monocytes were isolated from a human blood, using CD14+ magnetic beads. IL6, IL1-betta, and TNF-alpha were determined by ELISA. For macrophage phenotyping, a confocal microscopy was applied. "Sinus-150" was used for the generation of pulsed X-ray radiation (the absorbed dose was below 0.1 Gy, the pulse repetition frequency was 10 pulse/sec). The irradiation of mice by 0.1 Gy pulsed X-rays significantly inhibited the growth of primary tumor and reduced the number of metastatic colonies in the lung. Furthermore, the changes in macrophage phenotype and cytokine secretion were observed after repetitive pulsed X-ray radiation. Conclusion. Macrophages and tumor cells had a different response to a low-dose pulsed X-ray radiation. An activation of the immune system through changes of a macrophage phenotype can result in a significant antitumor effect of the low-dose repetitive pulsed X-ray radiation.

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Enhanced Cytotoxic Activity of Macrophages and Suppressed Tumor Metastases in Mice Irradiated with Low Doses of X- rays

Cited by 49 publications

References 24 publications

Effect of Low Doses of Low-Let Radiation on the Innate Anti-Tumor Reactions in Radioresistant and Radiosensitive Mice

Effect of Low Doses of Low-Let Radiation on the Innate Anti-Tumor Reactions in Radioresistant and Radiosensitive Mice

Immunological Mechanism of the Low-Dose Radiation-Induced Suppression of Cancer Metastases in a Mouse Model

Macrophage and tumor cell responses to repetitive pulsed X-ray radiation

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