Enhanced cytotoxicity of dimethylformamide by ultrasound in vitro

Jeffers, Russell; Feng, Ruo; Fowlkes, J. Brian; Hunt, J.W.; Cain, Charles A.

doi:10.1109/ultsym.1992.275855

Cited by 1 publication

(1 citation statement)

References 21 publications

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“…To probe this issue further, we identified solvents thought to minimally affect cell viability that could also be used to electrospin PCL, namely HFIP41 and repeated the layering procedure. Using HFIP, 80% of the attached cells were alive (Figure 10) initially compared to 50% when using DMF/chloroform, both of which have previously documented mammalian cell cytotoxicity 59, 60. In addition, the magnitude and decay of cell viability are analogous to that of unattached cells from the DMF/chloroform validating this to be a suitable solvent for cell layering.…”

Section: Resultsmentioning

confidence: 82%

Mammalian Cell Viability in Electrospun Composite Nanofiber Structures

Canbolat

Tang

Bernacki

et al. 2011

Macromolecular Bioscience

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Incorporation of mammalian cells into nanofibers (cell electrospinning) and multilayered cell-nanofiber structures (cell layering) via electrospinning are promising techniques for tissue engineering applications. We investigate the viability of 3T3-L1 mouse fibroblasts after incorporation into poly(vinyl alcohol) nanofibers and multilayering with poly(caprolactone) nanofibers and analyze the possible factors that affect cell viability. We observe that cells do not survive cell electrospinning but survive cell layering. Assessing the factors involved in cell electrospinning, we find that dehydration and fiber stretching are the main causes of cell death. In cell layering, the choice of solvent is critical, as residual solvent in the electrospun fibers could be detrimental to the cells.

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Section: Resultsmentioning

confidence: 82%