Enhanced decolourization of congo red dye under submerged fermentation (SMF) process by newly isolated Bacillus subtilis SPR42

Abstract

JANS Enhanced decolourization of congo red dye under submerged fermentation (SMF) process by newly isolated Bacillus subtilis SPR 42Baljeet Singh Saharan* and Poonam Ranga Department of Microbiology, Kurukshetra University, Kurukshetra-136 119 (Haryana), INDIA *Corresponding author. E-mail: baljeet@daad-alumni.de Abstract: Studies were carried out on the decolourization of textile azo dye using newly isolated aerobic bacterial culture. Among the 58 strains of aerobic bacteria isolated from soil contaminated with textile industry (Shivalik polymer Ltd. Faridabad) effluent, three showed remarkable ability in decolourizing the widely utilized azo dye (Congo Red). These strains also readily grew in and decolourized the high concentrations of dye (100 mgL -1 ). The aerobic bacterial isolate SPR 42 , was able to decolourize the Congo Red dye at a concentration of 100 mgL -1 upto 94% within 24 hrs at static conditions. The temperature and pH for optimum growth and activity of the isolate were reported as 37 o C and 8.5, respectively. The colorless bacterial biomass after decolourization suggested that decolourization was due to biodegradation, rather than inactive surface adsorption. Phenotypic characterization and phylogenetic analysis based on 16S rDNA sequence comparisons indicate that the strain SPR 42 identified as Bacillus subtilis. This isolate can be a potential strain for biological treatment of effluents of TPI (Textile Processing Industry).

Biotechnological Production of Polyhydroxyalkanoates: A Review on Trends and Latest Developments

“…Waste disposal creates toxic, growth inhibiting, and unfamiliar environmental conditions [2]. Even though, at such state of physiological stress, PHA producers have been reported to be residing with properties like degrading dyes, aromatic compounds, and left over organic matter discharge from industries [3][4][5][6][7]. This implausible behavioural property can be considered towards industrial production aspects and issues related to removal of pollutants like PAHs, pesticides, dyestuffs, and so forth.…”

Biological Decolorization of Reactive Textile Dye Yellow CRG

“…TS-2 showed high decolorization (77.9%) when grown at 35 º C temperature whereas slow decolorization was reported at 25ºC and 45ºC (Figure 4). Saharan and Ranga (2011) studied the Bacillus subtilis under different temperature for enhanced decolorization of Congo red dye in submerged fermentation. The cultured Bacillus subtilis under different temperature (25ºC.…”