Enhanced degradation of haloacid by heterologous expression in related <i>Burkholderia</i> species

Su, Xianbin; Deng, Liyu; Kong, Ka Fai; Tsang, Jwh

doi:10.1002/bit.24917

Cited by 11 publications

(6 citation statements)

References 56 publications

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“…Burkholderia bacteria have the ability to degrade environmental pollutants, B. xenovorans LB400 can decrease carcinogens (polychlorinated biphenyl, 2-aminophenol, Metallica) (Martıńez et al, 2007;Chirino et al, 2013;Reyes-Gallegos et al, 2016). The Burkholderia species MBA4 can enrich and bioremediate haloacids from soil (Su et al, 2013). Whether KJ-34 has the ability to decrease environmental pollutants is worthy of exploration.…”

Section: Discussionmentioning

confidence: 99%

Burkholderia gladioli strain KJ-34 exhibits broad-spectrum antifungal activity

Yang

Wang²,

Wan³

et al. 2023

Front. Plant Sci.

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IntroductionPlant pathogens are one of the major constraints on worldwide food production. The antibiotic properties of microbes identified as effective in managing plant pathogens are well documented.MethodsHere, we used antagonism experiments and untargeted metabolomics to isolate the potentially antifungal molecules produced by KJ-34.ResultsKJ-34 is a potential biocontrol bacterium isolated from the rhizosphere soil of rice and can fight multiple fungal pathogens (i.e. Ustilaginoidea virens, Alternaria solani, Fusarium oxysporum, Phytophthora capsica, Corynespora cassiicola). The favoured fermentation conditions are determined and the fermentation broth treatment can significantly inhibit the infection of Magnaporthe oryzae and Botryis cinerea. The fermentation broth suppression ratio is 75% and 82%, respectively. Fermentation broth treatment disrupted the spore germination and led to malformation of hyphae. Additionally, we found that the molecular weight of antifungal products were less than 1000 Da through semipermeable membranes on solid medium assay. To search the potentially antifungal molecules that produce by KJ-34, we used comparative and bioinformatics analyses of fermentation broth before and after optimization by mass spectrometry. Untargeted metabolomics analyses are presumed to have a library of antifungal agents including benzoylstaurosporine, morellin and scopolamine.DiscussionThese results suggest that KJ-34 produced various biological control agents to suppress multiple phytopathogenic fungi and showed a strong potential in the ecological technologies of prevention and protection.

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Section: Discussionmentioning

confidence: 99%

Burkholderia gladioli strain KJ-34 exhibits broad-spectrum antifungal activity

Yang

Wang²,

Wan³

et al. 2023

Front. Plant Sci.

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“…These compounds have genotoxic, mutagenic, cytotoxic, and tumorigenic effects in humans (Kim et al, 2020;Long et al, 2021;. In metabolically engineered Burkholderia species, the degradation activity of haloacetic acids can be increased by 4-8 times (Su et al, 2013). The bacterial degradation of haloacetic acids was found to be affected by water distribution system conditions, including pH, phosphate, total organic carbon and residual chlorine (Behbahani et al, 2018).…”

Section: Environmental Bioremediationmentioning

confidence: 99%

Mini Review: Advances in 2-Haloacid Dehalogenases

et al. 2021

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The 2-haloacid dehalogenases (EC 3.8.1.X) are industrially important enzymes that catalyze the cleavage of carbon–halogen bonds in 2-haloalkanoic acids, releasing halogen ions and producing corresponding 2-hydroxyl acids. These enzymes are of particular interest in environmental remediation and environmentally friendly synthesis of optically pure chiral compounds due to their ability to degrade a wide range of halogenated compounds with astonishing efficiency for enantiomer resolution. The 2-haloacid dehalogenases have been extensively studied with regard to their biochemical characterization, protein crystal structures, and catalytic mechanisms. This paper comprehensively reviews the source of isolation, classification, protein structures, reaction mechanisms, biochemical properties, and application of 2-haloacid dehalogenases; current trends and avenues for further development have also been included.

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“…It was also reported that MCA dehalogenase activity was found to be inducible, either in wild type of Pseudomonas sp. R1 (Ismail et al, 2008) or in its recombinant clone of Burkholderia spesies MBA4 (Su et al, 2013). Based on our result, we chose 1 mM of MCA concentration for initial optimization of inducer (IPTG) concentration.…”

Section: Growth Of E Coli Bl21 (De3)/pet-bcfd1 In Mca Containing Mediummentioning

confidence: 99%

Optimization of Monochloroacetic Acid Biodegradation by Recombinant E. coli BL21 (DE3)/pET-bcfd1 Carrying Haloacid Dehalogenase Gene from Bacillus cereus IndB1

Ratnaningsih

Ade

Putri

et al. 2021

Int. J. Renew. Energy Dev.

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In recent years, attention to microbial dehalogenase has continually increased due to its potential application, both in bioremediation and in the biosynthesis of fine chemicals. Many microbial recombinant strains carrying dehalogenase gene have been developed, particularly to increase the dehalogenase production and its quality. In this study, we aimed to find the optimum condition for the production of active haloacid dehalogenase by E. coli BL21 (DE3) harboring recombinant plasmid pET-bcfd1 that carried haloacid dehalogenase gene from Bacillus cereus IndB1 local strain. This would be examined by assessing the ability of whole cell life culture to degrade monochloroacetic acid (MCA) and quantifying the chloride ion released into the medium. Several variables were evaluated to find this optimal condition. We found that the best condition for MCA biodegradation using this recombinant clone was at 0.2 mM MCA, 10 μM of isopropyl β-D-1-thiogalactopyranoside (IPTG), 6 hours of pre-induction incubation at 37ºC with shaking, 2 hours IPTG induction at 30ºC with shaking, at pH 7 in Luria Bertani (LB) liquid medium without NaCl, which produced about 0.056 mM chloride ions. Inducer concentration, pre-induction incubation time and temperature, as well as induction time and temperature were apparent to be associated with the expression of the protein, while the MCA concentration and the pH of the medium influenced the ability of the recombinant E. coli BL21 (DE3)/pET-bcfd1 to grow in toxic environment. Our findings laid the foundation for exploration of dehalogenases from local Bacillus strains through genetic engineering for MCA biodegradation

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Enhanced degradation of haloacid by heterologous expression in related Burkholderia species

Cited by 11 publications

References 56 publications

Burkholderia gladioli strain KJ-34 exhibits broad-spectrum antifungal activity

Burkholderia gladioli strain KJ-34 exhibits broad-spectrum antifungal activity

Mini Review: Advances in 2-Haloacid Dehalogenases

Optimization of Monochloroacetic Acid Biodegradation by Recombinant E. coli BL21 (DE3)/pET-bcfd1 Carrying Haloacid Dehalogenase Gene from Bacillus cereus IndB1

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