Enhanced Degradation of the Fungicide Vinclozolin: Isolation and Characterisation of a Responsible Organism

Abstract

Enhanced degradation of the fungicide vinclozolin was stimulated by multiple successive applications to a soil without any history of previous pesticide input. A vinclozolin-degrading bacterium isolated from this soil was identified as a strain of Pseudomonas putida. This organism metabolised vinclozolin as a source of carbon, but it would neither grow with nor transform any other closely related dicarboximide fungicides nor the degradation product, 3,5-DCA. The degradation of vinclozolin by cultures of P. putida St-1 was investigated under various culture conditions; biodegradation was optimal at 23"C, pH 6-5 and inoculum densities of lo7 cells ml-' but cultures would grow from as little as 100 cells ml-l. Amendments of the vinclozolin-degrading isolate to soil previously untreated with the fungicide caused rapid degradation of applied vinclozolin, whereas amendments of boiled cells, or viable cells grown in the absence of vinclozolin, produced no discernible effect on the rate of vinclozolin degradation.

Biodegradation kinetics of the benzimidazole fungicide thiophanate-methyl by bacteria isolated from loamy sand soil

“…It's a reason that pesticide degradation by bacteria and fungi has received much attention (Cain and Mitchell 1996;Mercadier et al 1998;Pai et al 2001;Rama and Ligy 2009). A number of bacterial species are known to degrade benzimidazole structures and most of them were isolated from fungicide contaminated soils.…”

Factors in Geotropospheric Particle–Gas Transport of Semivolatile Organic Compounds

“…A strain of Corynebacterim species was found to degrade vinclozolin to M1-butenoic acid, but did not mediate the M2-enanilide reaction. Pseudomonas putida metabolizes vinclozolin as a food carbon source, but has not been observed to degrade the dicarboximide compounds (Cain and Mitchell, 1996). Other species of Pseudomonas have degraded the dicarboximide iprodione (Mercadier, et al, 1997).…”

“…The total dicarboximide flux was approximately equal for sterile and non-sterile soil, but the chemical speciation was different. The post-application flux of the parent compound vinclozolin was greater in the first 600min from sterilized soil than the parent flux from non-sterile soil under these experimental conditions, similar to previous findings in microbial studies (Mercadier, et al 1998;Mercadier, et al 1997;Ou et al, 1997;Cain and Mitchell 1996).…”

Allochthonous DOC in floodplain rivers: identifying sources using solid phase microextraction with gas chromatography

“…An algal culture was grown from 1.5 mL inoculum (filtered water from Kurrajong Billabong) in 1500 mL of basal salts medium (after Cain and Mitchell, 1996) in a 2 L Schott bottle. The Schott bottle was loosely capped and allowed to stand in a well-lit position at ambient temperature (20 -308C) and out of direct sunlight for 9 months.…”