Enhanced denitrification of sewage via bio-microcapsules embedding heterotrophic nitrification-aerobic denitrification bacteria Acinetobacter pittii SY9 and corn cob

Shi, Yunqi; Hu, Yongyou; Wang, Guobin; Xie, Jieyun; Zhu, Xiaoqiang

doi:10.1016/j.biortech.2022.127260

Cited by 21 publications

(6 citation statements)

References 32 publications

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“…The average concentrations were 823.18 mg/L, 276.50 mg/L, and 92.46 respectively, corresponding to the HRTs of 7, 24, and 16 h. This pattern aligns with the sequence in which the HRTs were evaluated during the operation time of the columns, indicating that COD decreased as the experiment progressed. These results are consistent with findings reported by other researchers, where carbon release was greater at the beginning of the experiment and decreased over time [21,70,71]. This increase in COD is attributed to the soluble organic carbon released through autolysis, which is rapidly consumed by microorganisms.…”

Section: • Characteristics Of Carbon Releasesupporting

confidence: 93%

Enhanced Biological Nitrate Removal from Groundwater in Humid Tropical Regions Using Corn Cob-Based Permeable Reactive Barriers: A Case Study from Panama

Sánchez Hidalgo,

Ortega,

Deago

2024

Water

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Nitrate contamination in groundwater is a global concern due to its widespread presence and consequential social, environmental, and economic ramifications. This study investigates the efficacy of biological denitrification in a humid tropical setting, utilizing corn cob in batch and column tests to assess nitrate removal under varying conditions. Batch tests demonstrated the nitrate removal efficiencies of 93.14%, 91.58%, 90.77%, and 98.74% for initial concentrations of 22.18 ± 2.82 mg/L, 27.3 mg/L, 69.1 ± 1.2 mg/L and 115.08 ± 1.88 mg/L, respectively. In the column test, the removal efficiency was 99.86%, 87.13%, and 74%, and the denitrification rate was 32.82, 53.43, and 83.53 mg NO3−-N/L d, for a hydraulic retention time (HRT) of 24 h, 16 h, and 7 h, respectively. Predominantly, nitrate removal occurred via biological denitrification, particularly favoring a 24 h HRT. The corn cob effectively removed high nitrate concentrations of up to 115 mg NO3−-N/L. Scanning electron microscopy and Fourier transform infrared spectroscopy revealed surface characteristic changes of the carbon source pre- and post-denitrification. This research sheds light on the potential of biological denitrification using corn cob in humid tropical environments, offering a promising avenue for addressing nitrate contamination challenges in groundwater systems.

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Section: • Characteristics Of Carbon Releasesupporting

confidence: 93%

Enhanced Biological Nitrate Removal from Groundwater in Humid Tropical Regions Using Corn Cob-Based Permeable Reactive Barriers: A Case Study from Panama

Sánchez Hidalgo,

Ortega,

Deago

2024

Water

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“…This may be attributable to the complexity of sewage composition, which may hinder microbial osmoregulation and impede intracellular enzymatic activity, thereby exerting irreversible effects on strain growth and metabolism. In the later stages, enhancing the denitrification capacity of strain HS2 can be achieved through techniques such as the encapsulation of the microorganism in biopolymeric microcapsules along with organic substrates [ 46 , 47 ]. Additionally, the co-cultivation of fungi and bacteria could be employed to augment the denitrification efficiency of sewage [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Denitrification Characteristics of the Low-Temperature Tolerant Denitrification Strain Achromobacter spiritinus HS2 and Its Application

Gao,

Zhang,

et al. 2024

Microorganisms

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The low-temperature environment significantly inhibits the growth and metabolism of denitrifying bacteria, leading to an excessive concentration of ammonia nitrogen and total nitrogen in sewage treatment plants during the cold season. In this study, an efficient denitrifying strain of heterotrophic nitrification–aerobic denitrification (HN–AD) bacteria named HS2 was isolated and screened from industrial sewage of a chemical factory in Inner Mongolia at 8 °C. The strain was confirmed to be Achromobacter spiritinus, a colorless rod-shaped bacterium. When cultured with sodium succinate as the carbon source, a carbon-to-nitrogen ratio of 20–30, a shaking rate of 150–180 r/min, and an initial pH of 6–10, the strain HS2 exhibited excellent nitrogen removal at 8 °C. Through the results of whole-genome sequencing, gene amplification, and gas product detection, the strain HS2 was determined to possess key enzyme genes in both nitrification and denitrification pathways, suggesting a HN–AD pathway of NH4+-N → NH2OH → NO2−N → NO → N2O → N2. At 8 °C, the strain HS2 could completely remove ammonia nitrogen from industrial sewage with an initial concentration of 127.23 mg/L. Microbial species diversity analysis of the final sewage confirmed Achromobacter sp. as the dominant genus, which indicated that the low-temperature denitrifying strain HS2 plays an important role in nitrogen removal in actual low-temperature sewage.

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“…The whole gene sequencing and gene amplification were not deep enough to investigate the nitrogen metabolism pathway of strain HS2, and the nitrogen transformation pathway could be investigated by using isotope tracer assay to measure nitrogenous compounds, or qPCR to measure the expression of genes related to the HN-AD process [50]. Ozkaraova et al [32]studied organic substrates such as peanut, walnut and almond shells and luffa sponge enhanced denitrification in wastewater treatment. The use of low-temperature biochar enhanced the nitrogen removal capacity of combinatorial bacteria in the study by Jiang et al [9].…”

Section: Discussionmentioning

confidence: 99%

Denitrification Characteristics of the Low-Temperature Tolerant Denitrification Strain <em>Achromobacter spiritinus</em> HS2 and Its Application

Gao,

Zhang,

et al. 2024

Preprint

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The low-temperature environment significantly inhibits the growth and metabolism of denitrifying bacteria, leading to an excessive concentration of ammonia nitrogen and total nitrogen in sewage treatment plants during the cold season. In this study, an efficient denitrifying strain of heterotrophic nitrification–aerobic denitrification(HN-AD) bacteria named HS2 was isolated and screened from industrial sewage of a chemical factory in Inner Mongolia at 8°C. The strain was confirmed to be Achromobacter spiritinus, a colorless rod-shaped bacterium. When cultured with sodium succinate as the carbon source, a carbon-to-nitrogen ratio of 20-30, a shaking rate of 150-180 r/min, and the initial pH of 6-10, the Strain HS2 exhibited excellent nitrogen removal at 8°C. Through the results of whole-genome sequencing, gene amplification, and gas product detection, the Strain HS2 was determined to possess key enzyme genes in both nitrification and denitrification pathways, suggesting a HN-AD pathway of NH4+-N→NH2OH→NO2--N→NO→N2O→N2. At 8°C, the Strain HS2 could completely remove ammonia nitrogen from industrial sewage with an initial concentration of 127.23 mg/L. Microbial species diversity analysis of the final sewage confirmed Achromobacter sp. as the dominant genus, this indicated that the low-temperature denitrifying strain HS2 plays an important role in nitrogen removal of actual low-temperature sewage.

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Enhanced denitrification of sewage via bio-microcapsules embedding heterotrophic nitrification-aerobic denitrification bacteria Acinetobacter pittii SY9 and corn cob

Cited by 21 publications

References 32 publications

Enhanced Biological Nitrate Removal from Groundwater in Humid Tropical Regions Using Corn Cob-Based Permeable Reactive Barriers: A Case Study from Panama

Enhanced Biological Nitrate Removal from Groundwater in Humid Tropical Regions Using Corn Cob-Based Permeable Reactive Barriers: A Case Study from Panama

Denitrification Characteristics of the Low-Temperature Tolerant Denitrification Strain Achromobacter spiritinus HS2 and Its Application

Denitrification Characteristics of the Low-Temperature Tolerant Denitrification Strain <em>Achromobacter spiritinus</em> HS2 and Its Application

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