Enhanced depolluting capabilities of microbial bioelectrochemical systems by synthetic biology

Tao, Le; Song, Maoyong; Jiang, Guibin

doi:10.1016/j.synbio.2023.05.005

Cited by 5 publications

(3 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bioelectrochemical systems (BESs) are an emerging sustainable technology for the simultaneous production of energy and wastewater treatment that has significantly increased during the past fifteen years [ 1 , 2 ]. Applications of BESs include microbial desalination cells (MDCs) for water desalting and microbial fuel cells (MFCs), which use microbial metabolism to generate energy from organic materials.…”

Section: Introductionmentioning

confidence: 99%

“…In addition to these natural characteristics, Pseudomonas can be easily modified with several synthetic engineering techniques and tools that can be useful to increase productivity in MFCs, such as increasing power density and current density [ 23 , 24 , 25 , 26 , 27 , 28 ]. Moreover, by synthetic biology techniques, EET kinetics can be improved, which is useful for microbial anodic electro-respiration and to promote pollutant removal at both the anode and cathode [ 1 ]. Additionally, it is possible to stimulate the growth of a biofilm on the electrode, increasing the number of microorganisms that can be used for subsequent catalytic reactions [ 38 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Synthetic Biology Toolkit for a New Species of Pseudomonas Promissory for Electricity Generation in Microbial Fuel Cells

Rabiço,

Pedrino,

Narcizo

et al. 2023

Microorganisms

View full text Add to dashboard Cite

Microbial fuel cells (MFCs) offer sustainable solutions for various biotechnological applications and are a crucial area of research in biotechnology. MFCs can effectively treat various refuse, such as wastewater and biodiesel waste by decomposing organic matter and generating electricity. Certain Pseudomonas species possess extracellular electron transfer (EET) pathways, enabling them to transfer electrons from organic compounds to the MFC’s anode. Moreover, Pseudomonas species can grow under low-oxygen conditions, which is advantageous considering that the electron transfer process in an MFC typically leads to reduced oxygen levels at the anode. This study focuses on evaluating MFCs inoculated with a new Pseudomonas species grown with 1 g.L−1 glycerol, a common byproduct of biodiesel production. Pseudomonas sp. BJa5 exhibited a maximum power density of 39 mW.m−2. Also, the observed voltammograms and genome analysis indicate the potential production of novel redox mediators by BJa5. Additionally, we investigated the bacterium’s potential as a synthetic biology non-model chassis. Through testing various genetic parts, including constitutive promoters, replication origins and cargos using pSEVA vectors as a scaffold, we assessed the bacterium’s suitability. Overall, our findings offer valuable insights into utilizing Pseudomonas spp. BJa5 as a novel chassis for MFCs. Synthetic biology approaches can further enhance the performance of this bacterium in MFCs, providing avenues for improvement.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Synthetic Biology Toolkit for a New Species of Pseudomonas Promissory for Electricity Generation in Microbial Fuel Cells

Rabiço,

Pedrino,

Narcizo

et al. 2023

Microorganisms

View full text Add to dashboard Cite

show abstract

“…In addition to these natural characteristics, Pseudomonas can be easily modified with several synthetic engineering techniques and tools that can be useful to increase productivity in MFCs, such as increasing power density and current density [23][24][25][26][27][28]. Moreover, by synthetic biology techniques, EET kinetics can be improved, which is useful for microbial anodic electro-respiration and to promote pollutant removal at both the anode and cathode [1]. Additionally, it is possible to stimulate the growth of a biofilm on the electrode, increasing the number of microorganisms that can be used for subsequent catalytic reactions [38].…”

Section: Introductionmentioning

confidence: 99%

Synthetic Biology Toolkit for a New Species of <em>Pseudomonas</em> Promissory for Electricity Generation in Microbial Fuel Cells

Rabiço,

Pedrino,

Narcizo

et al. 2023

Preprint

View full text Add to dashboard Cite

Microbial fuel cells (MFCs) offer sustainable solutions for various biotechnological applications and are a crucial area of research in biotechnology. MFCs can effectively treat wastewater and biodiesel waste by decomposing organic matter and generating electricity. Certain Pseudomonas species possess extracellular electron transfer (EET) pathways, enabling them to transfer electrons from organic compounds to the MFC's anode. Moreover, Pseudomonas species can grow under low oxygen conditions, which is advantageous considering that the electron transfer process in an MFC typically leads to reduced oxygen levels at the anode. This study focuses on evaluating MFCs inoculated with a new Pseudomonas species, grown with 1 g.L-1 glycerol, a common byproduct of biodiesel production. Pseudomonas sp. BJa5 exhibited a maximum power density of 39 mW.m-2. Also, the observed voltammograms and genome analysis indicate the potential production of novel redox mediators by BJa5. Additionally, we investigated the bacterium's potential as a synthetic biology non model chassis. Through testing various genetic parts, including constitutive promoters, replication origins, and cargos using pSEVA vectors as a scaffold, we assessed the bacterium's suitability. Overall, our findings offer valuable insights into utilizing Pseudomonas spp. BJa5 as a novel chassis for MFCs. Synthetic biology approaches can further enhance the performance of this bacterium in MFCs, providing avenues for improvement.

show abstract

Applications of Microbial Electrolysis Cells in Anaerobic Digestion

Zulkornain,

Mohamed,

Salehmin

2024

Sustainable Materials and Technology

View full text Add to dashboard Cite

Enhanced depolluting capabilities of microbial bioelectrochemical systems by synthetic biology

Cited by 5 publications

References 39 publications

Synthetic Biology Toolkit for a New Species of Pseudomonas Promissory for Electricity Generation in Microbial Fuel Cells

Synthetic Biology Toolkit for a New Species of Pseudomonas Promissory for Electricity Generation in Microbial Fuel Cells

Synthetic Biology Toolkit for a New Species of <em>Pseudomonas</em> Promissory for Electricity Generation in Microbial Fuel Cells

Applications of Microbial Electrolysis Cells in Anaerobic Digestion

Contact Info

Product

Resources

About