Enhanced Differentiation of Wharton’s Jelly-Derived Mesenchymal Stem Cells in Insulin-Producing Cells by the Extract of Nigella sativa Seeds

Ranjbaran, Hossein; Abediankenari, Saeid; Amiri, Marzei Momeninezhad

doi:10.5812/ircmj.62111

Cited by 16 publications

(16 citation statements)

References 15 publications

(14 reference statements)

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“…MSCs can differentiate into insulin-producing cells (IPCs) both in vitro and in vivo, which underlies their potential for treating DM [ 98 ]. The transplantation of IPCs derived from different types of MSCs in vitro is effective in regulating blood glucose levels in experimental diabetic animals [ 99 , 100 , 101 ].…”

Section: Preclinical and Clinical Approaches To Msc In Diabetes-induced Cardiac Complicationsmentioning

confidence: 99%

Mesenchymal Stem Cell Therapy in Diabetic Cardiomyopathy

Gonçalves

Vasques

et al. 2022

Cells

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The incidence and prevalence of diabetes mellitus (DM) are increasing worldwide, and the resulting cardiac complications are the leading cause of death. Among these complications is diabetes-induced cardiomyopathy (DCM), which is the consequence of a pro-inflammatory condition, oxidative stress and fibrosis caused by hyperglycemia. Cardiac remodeling will lead to an imbalance in cell survival and death, which can promote cardiac dysfunction. Since the conventional treatment of DM generally does not address the prevention of cardiac remodeling, it is important to develop new alternatives for the treatment of cardiovascular complications induced by DM. Thus, therapy with mesenchymal stem cells has been shown to be a promising approach for the prevention of DCM because of their anti-apoptotic, anti-fibrotic and anti-inflammatory effects, which could improve cardiac function in patients with DM.

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Section: Preclinical and Clinical Approaches To Msc In Diabetes-induced Cardiac Complicationsmentioning

confidence: 99%

Mesenchymal Stem Cell Therapy in Diabetic Cardiomyopathy

Gonçalves

Vasques

et al. 2022

Cells

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“…However, spontaneous differentiation efficiency is extremely low [14]. Numerous studies have chemically [15][16][17] or genetically [18][19][20] induced MSCs into IPCs by effectively applying small molecules or genetic engineering to improve differentiation efficiency. Genetic engineering schemes are highly efficient, expensive, cumbersome, and time-consuming, and most of them use viruses as vectors, which are teratogenic and form tumors.…”

Section: Ipcs Transplantationmentioning

confidence: 99%

Mesenchymal Stem Cells: An Excellent Candidate for the Treatment of Diabetes Mellitus

Huang

Liu

2021

International Journal of Endocrinology

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Mesenchymal stem cells (MSCs) are adult stem cells (ASCs) known for repairing damaged cells, exerting anti-inflammatory responses and producing immunoregulatory effects that can be significantly induced into insulin-producing cells (IPCs), providing an inexhaustible supply of functional β cells for cell replacement therapy and disease modeling for diabetes. MSC therapy may be the most promising strategy for diabetes mellitus because of these significant merits. In this paper, we focused on MSC therapy for diabetes.

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“…Similarly, stored umbilical cord-derived MSCs can serve as an excellent autologous source for their donors in case of a future need. Recently, there is an increasing interest in the use of Wharton’s jelly-derived MSCs to generate IPCs [ 58 , 59 ]. It provides an unlimited source for MSCs without ethical considerations.…”

Section: Mesenchymal Stem/stromal Cellsmentioning

confidence: 99%

From Mesenchymal Stromal/Stem Cells to Insulin-Producing Cells: Progress and Challenges

Ghoneim

Refaie

Elbassiouny

et al. 2020

Stem Cell Rev and Rep

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Mesenchymal stromal cells (MSCs) are an attractive option for cell therapy for type 1 diabetes mellitus (DM). These cells can be obtained from many sources, but bone marrow and adipose tissue are the most studied. MSCs have distinct advantages since they are nonteratogenic, nonimmunogenic and have immunomodulatory functions. Insulin-producing cells (IPCs) can be generated from MSCs by gene transfection, gene editing or directed differentiation. For directed differentiation, MSCs are usually cultured in a glucose-rich medium with various growth and activation factors. The resulting IPCs can control chemically-induced diabetes in immune-deficient mice. These findings are comparable to those obtained from pluripotent cells. PD-L1 and PD-L2 expression by MSCs is upregulated under inflammatory conditions. Immunomodulation occurs due to the interaction between these ligands and PD-1 receptors on T lymphocytes. If this function is maintained after differentiation, life-long immunosuppression or encapsulation could be avoided. In the clinical setting, two sites can be used for transplantation of IPCs: the subcutaneous tissue and the omentum. A 2-stage procedure is required for the former and a laparoscopic procedure for the latter. For either site, cells should be transplanted within a scaffold, preferably one from fibrin. Several questions remain unanswered. Will the transplanted cells be affected by the antibodies involved in the pathogenesis of type 1 DM? What is the functional longevity of these cells following their transplantation? These issues have to be addressed before clinical translation is attempted.

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Enhanced Differentiation of Wharton’s Jelly-Derived Mesenchymal Stem Cells in Insulin-Producing Cells by the Extract of Nigella sativa Seeds

Cited by 16 publications

References 15 publications

Mesenchymal Stem Cell Therapy in Diabetic Cardiomyopathy

Mesenchymal Stem Cell Therapy in Diabetic Cardiomyopathy

Mesenchymal Stem Cells: An Excellent Candidate for the Treatment of Diabetes Mellitus

From Mesenchymal Stromal/Stem Cells to Insulin-Producing Cells: Progress and Challenges

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