Enhanced Dissolved Organic Matter Recovery from Saltwater Samples with Electrodialysis

Chambers, Luke R.; Ingall, Ellery D.; Saad, Emily M.; Longo, Amelia F.; Takeuchi, Masayuki; Tang, Yuanzhi; Benítez-Nelson, Claudia R.; Haley, Sheean T.; Dyhrman, Sonya T.; Brandes, Jay; Stubbins, Aron

doi:10.1007/s10498-016-9306-2

Cited by 12 publications

(30 citation statements)

References 39 publications

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“…This implies the isolation bias associated with ED was likely related to compound charge and mass. This was similar to that found by Chambers et al (). Linear regression analyses found a relationship ( α = 0.05; R 2 = 0.938) between DOP recovery and MW (Fig.…”

Section: Materials and Proceduressupporting

confidence: 93%

“…Previous studies utilizing ED/RO for environmental DOM isolation (determined as DOC recovery) have reported comparable recoveries: 76% ± 15%, 75% ± 12%, 59% ± 12%, 66% ± 3%, 67% ± 15%, 76% ± 7%, and 71% ± 7% (respectively, Vetter et al ; Gurtler et al and Koprivnjak et al ; Young and Ingall ; Chen et al ; Green et al ; Chambers et al ; Saad et al ). Differences in recovery efficiency are likely impacted by processing methodology (averages given by study), but consistent variability (also observed with SPE) is largely attributed to the inherent heterogeneity of DOM composition (Hertkorn et al ; Minor et al ).…”

Section: Discussionmentioning

confidence: 84%

“…Differences in recovery efficiency are likely impacted by processing methodology (averages given by study), but consistent variability (also observed with SPE) is largely attributed to the inherent heterogeneity of DOM composition (Hertkorn et al ; Minor et al ). With respect toward isolation biases due to molecular structure, Chambers et al () found overall similar recovery biases, but found greater bias of select DOC compounds with charge (∼ 30%, EDTA vs. vitamin B 12 ) than by size (∼ 10%, glucose‐6 vs. vitamin B 12 ). Bulk DOC : DOP ratios were found to be minimally impacted during the isolation process, further reflecting minimal isolation biases (Table ).…”

Section: Discussionmentioning

confidence: 90%

“…The secondary objective of this assessment was to examine potential biases to DOP composition and characterization introduced by solution 31 P‐NMR. The assessment further touched on comparisons with DOM isolation studies (Green et al ; Chambers et al ).…”

mentioning

confidence: 99%

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Isolation and molecular characterization of dissolved organic phosphorus using electrodialysis‐reverse osmosis and solution ³¹P‐NMR

Bell

Pellechia

Chambers

et al. 2017

Limnology & Ocean Methods

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The composition of dissolved organic phosphorus (DOP), which directly impacts its biological and chemical reactivity, remains poorly constrained due to methodological and operational challenges. A series of compound‐specific isolation experiments and bulk estuarine seawater isolations were conducted to assess the ability of coupled electrodialysis (ED) and reverse osmosis (RO) to isolate DOP from seawater for molecular characterization using solution 31P‐NMR analysis. These isolations were carried out using a recently developed small‐volume (24 L) ED/RO system. Compound‐specific isolation experiments (n = 7) averaged 64.1% ± 9.2% for final DOP recovery, while recoveries for estuarine seawater (n = 7) were on average 83.8% ± 10.7%. Nearly complete mass balance of DOP was observed for the compound‐specific isolations (88.1% ± 11.2%) with ED responsible for the majority of DOP loss (16% ± 5%). Isolation recovery was impacted by compound molecular weight rather than charge‐to‐size ratio. Isolation bias between bulk dissolved organic carbon and DOP was negligible. Solution 31P‐NMR confirmed that ED/RO isolation does not impact molecular integrity or DOP composition. In addition, solution 31P‐NMR accurately estimated several components (phosphonomethyl‐glycine, D‐glucose‐6‐phosphate, L‐α‐phosphatidylcholine) of an artificial complex DOP matrix. However, we suggest further refinements for ED/RO operation and significant caveats remain for 31P‐NMR sample preparation and the interpretation of 31P‐NMR spectra. Hydrolysis from NaOH dissolution was found to impact the ability to accurately resolve diester composition (ca. 40% underestimation) and discrimination between terminal and central phosphoanhydride atoms. Nonetheless, our results describe a viable option for the isolation and molecular characterization of marine DOP.

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Section: Materials and Proceduressupporting

confidence: 93%

Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 90%

mentioning

confidence: 99%

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Isolation and molecular characterization of dissolved organic phosphorus using electrodialysis‐reverse osmosis and solution ³¹P‐NMR

Bell

Pellechia

Chambers

et al. 2017

Limnology & Ocean Methods

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“…Chambers et al (2016) describe an improved method coupling different techniques to recover dissolved organic matter for improving its compositional characterization. Lastly, Komoda et al (2016) …”

Section: Contributions By Colleagues To This Special Issuementioning

confidence: 99%

A Tribute to Rick and Debbie Jahnke: From Deep Sea Pore Water to Coastal Permeable Sediments-Contributions that Cover the Oceans

Shaw

Emerson

Windom³

2016

Aquat Geochem

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Development of the Miniature Robotic Electrodialysis (MR ED) System for Small-Scale Desalting of Liquid Samples with Recovery of Organics

Bryson

Ingall

Hanna

et al. 2022

Preprint

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While liquid environments with high salt content are of broad interest to the Earth and Planetary Science communities, instruments face challenges in detecting organics in hypersaline samples due to the effects of salts. Therefore, technology to desalt samples before analysis by these instruments would be enabling for liquid sampling on missions to Mars or ocean worlds. Electrodialysis (ED) removes salt from aqueous solutions by applying an electric potential across a series of ionselective membranes, and is demonstrated to retain a significant percentage of dissolved organic molecules (DOM) in marine samples. However, current electrodialysis systems used for DOM recovery are too large for deployment on missions or for use in terrestrial fieldwork. Here we present the design and evaluation of the Minature Robotic Electrodialysis (MR ED) system, which is approximately 1/20th the size of heritage instruments and processes as little as 50 mL of sample at a time. We present tests of the instrument efficiency and DOM recovery using lab-created solutions as well as natural samples taken from an estuary of the Skidaway River (Savannah, GA) and from South Bay Saltworks (San Diego, CA). Our results show that the MR ED system removed 97-99% of the salts in most samples, with an average DOC recovery range from 53 to 77%, achieving similar capability to tabletop instruments. This work both demonstrates MR ED as a possible field instrument and increases the technology readiness level of miniaturized electrodialysis systems for future missions.

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Enhanced Dissolved Organic Matter Recovery from Saltwater Samples with Electrodialysis

Cited by 12 publications

References 39 publications

Isolation and molecular characterization of dissolved organic phosphorus using electrodialysis‐reverse osmosis and solution ³¹P‐NMR

Isolation and molecular characterization of dissolved organic phosphorus using electrodialysis‐reverse osmosis and solution ³¹P‐NMR

A Tribute to Rick and Debbie Jahnke: From Deep Sea Pore Water to Coastal Permeable Sediments-Contributions that Cover the Oceans

Development of the Miniature Robotic Electrodialysis (MR ED) System for Small-Scale Desalting of Liquid Samples with Recovery of Organics

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Enhanced Dissolved Organic Matter Recovery from Saltwater Samples with Electrodialysis

Cited by 12 publications

References 39 publications

Isolation and molecular characterization of dissolved organic phosphorus using electrodialysis‐reverse osmosis and solution 31P‐NMR

Isolation and molecular characterization of dissolved organic phosphorus using electrodialysis‐reverse osmosis and solution 31P‐NMR

A Tribute to Rick and Debbie Jahnke: From Deep Sea Pore Water to Coastal Permeable Sediments-Contributions that Cover the Oceans

Development of the Miniature Robotic Electrodialysis (MR ED) System for Small-Scale Desalting of Liquid Samples with Recovery of Organics

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Product

Resources

About

Isolation and molecular characterization of dissolved organic phosphorus using electrodialysis‐reverse osmosis and solution ³¹P‐NMR

Isolation and molecular characterization of dissolved organic phosphorus using electrodialysis‐reverse osmosis and solution ³¹P‐NMR