Enhanced efficacy with reduced toxicity of chemotherapy drug 5-fluorouracil by synergistic treatment with Abnormal Savda Munziq from Uyghur medicine

Abstract

BackgroundAbnormal Savda Munziq (ASMq) is a traditional prescription in Uyghur Medicine, and its treatment of complex diseases such as tumors and asthma has been proven to be effective in Uyghur medical clinical practice. The efficacy-enhancing and toxicity-reducing properties of ASMq were studied on mice with transplanted cervical cancer (U27) tumors, which were treated with 5-fluorouracil (5-FU) in this work.MethodsTo investigate the synergistic effect of ASMq and 5-FU on U27 cells, inhibitory effects on cell proliferation were determined through a MTT assay. 48 Kunming mice which were randomly divided in to 6 groups: control group, model group, 5-FU group, 5-FU combine with ASMq low-dose group, 5-FU combine with ASMq medium-dose group, and 5-FU combine with ASMq high- dose group, the inhibition rate of the tumor, the viscera indexes, and the content of serum tumor necrosis factor-α (TNF-α), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined. The expression levels of transforming growth factor-β1 (TGF-β1) and human papillomavirus type 16 E2 (HPV16 E2) protein were assessed by Western blot. Pathological changes in the liver were observed.ResultThe inhibition rates of tumors, the 5-FU + ASMq.H group(80.64%), 5-FU + ASMq.M group (90.67%), 5-FU + ASMq.L group (72.03%) and 5-FU group (66.89%), clearly indicated that the effects of tumor inhibition. The thymus index and spleen index were increased, and the serum concentration of TNF-α increased while ALT and AST concentrations were decreased, and TNF-α protein expression were increased while TGF-β1 and HPV16 E2 were decreased. ASMq might can improve livers central vein hyperemia and interstitial edema, and preserve the radial structure of the hepatic cords.ConclusionsThe results suggested that ASMq might reduce toxicity and enhance the efficacy of the chemotherapeutic drug 5-fluorouracil in the treatment of cervical carcinoma.

Evaluating the mucoprotective effects of glycyrrhizic acid-loaded polymeric nanoparticles in a murine model of 5-fluorouracil-induced intestinal mucositis via suppression of inflammatory mediators and oxidative stress

“…In previous animal experimental models, 5-FU signi cantly affect weight of spleen, kidney and to lesser extent liver (Gelen et al 2018;Whittaker et al 2016;Yang et al 2017). In the current experiment, visceral organs weight assessment indicated that 5-FU decline splenic weight about 66% and kidney weight about 32%, as compared to the normal control (Fig.…”

Evaluating the Mucoprotective Effects of Glycyrrhizic Acid Loaded Polymeric Nanoparticles Against 5-Fluorouracil Induced Intestinal Mucositis in Murine Model via Suppression of Inflammatory Mediators and Oxidative Stress

“…In previous animal experimental models, 5-FU signi cantly affect weight of spleen, kidney and to lesser extent liver (Gelen et al 2018;Whittaker et al 2016;Yang et al 2017). In the current experiment, visceral organs weight assessment indicated that 5-FU decline splenic weight about 66% and kidney weight about 32%, as compared to the normal control (Fig.…”

Effect of monosodium glutamate on serum sex hormones and uterine histology in female rats along with its molecular docking and in-silico toxicity

“…After overnight incubation in a CO 2 incubator at 37 °C, cells were treated with a 2-fold serial range of concentrations of MSG (100–0.78 μg/ml) followed by incubation for 24, 48, and 72 h. After that, MTT (5 mg/ml) was added to each well and re-incubated for 4 h. Formazan crystals were dissolved in DMSO, and optical density was recorded using a microplate reader at 570 nm. The cell viability percentage was estimated in comparison to the control [ 51 ]. The assay was carried out in triplicates measurement and at least three independent experiments.…”