Enhanced efficiency of genetic programming toward cardiomyocyte creation through topographical cues

Morez, Constant; Noseda, Michela; Paiva, Marta; Belian, Elisa; Schneider, Michael; Stevens, Molly M.

doi:10.1016/j.biomaterials.2015.07.063

Cited by 86 publications

(97 citation statements)

References 61 publications

(92 reference statements)

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“…In-vitro models indicate that muscle progenitor cells utilize alignment cues during myogenesis [51, 52]. Similar alignment-sensitive responses have been reported for other cells, including cardiac muscle and tendons [53, 54]. Yet, while the many in-vitro alignment studies support the motivation of aligned regenerative scaffolds, we recognize that in-vivo evidence indicating that scaffold alignment is critical to muscle implant success does not exist.…”

Section: Discussionsupporting

confidence: 58%

The characterization of decellularized human skeletal muscle as a blueprint for mimetic scaffolds

Wilson

Terlouw

Roberts

et al. 2016

J Mater Sci: Mater Med

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The use of decellularized skeletal muscle (DSM) as a cell substrate and scaffold for the repair of volumetric muscle loss injuries has shown therapeutic promise. The performance of DSM materials motivated our interest in exploring the chemical and physical properties of this promising material. We suggest that these properties could serve as a blueprint for the development of next generation engineered materials with DSM mimetic properties. In this study, whole human lower limb rectus femoris (n=10) and upper limb supraspinatus muscle samples (n=10) were collected from both male and female tissue donors. Skeletal muscle samples were decellularized and nine property values, capturing key compositional, architectural, and mechanical properties, were measured and statistically analyzed. Mean values for each property were determined across muscle types and genders. Additionally, the influence of muscle type (upper versus lower limb) and donor gender (male versus female) on each of the DSM material properties was examined. The data suggests that DSM materials prepared from lower limb rectus femoris samples have an increased modulus and contain a higher collagen content then upper limb supraspinatus muscles. Specifically, lower limb rectus femoris DSM material modulus and collagen content was approximately twice that of lower limb supraspinatus DSM samples. While muscle type did show some influence on material properties, we did not find significant trends related to gender. The material properties reported herein may be used as a blueprint for the data-driven design of next generation engineered scaffolds with muscle mimetic properties, as well as inputs for computational and physical models of skeletal muscle.

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Section: Discussionsupporting

confidence: 58%

The characterization of decellularized human skeletal muscle as a blueprint for mimetic scaffolds

Wilson

Terlouw

Roberts

et al. 2016

J Mater Sci: Mater Med

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show abstract

“…39,40 Similar alignment-sensitive responses have been reported for other cells, including cardiac muscle and tendons. 41,42 Yet, while the many in vitro alignment studies support the motivation of aligned regenerative scaffolds, we recognize that in vivo evidence indicating that scaffold alignment is critical to muscle implant success does not yet exist. The lack of in vivo data supporting the importance of scaffold alignment in muscle regenerative therapies would appear to motivate future studies.…”

Section: Decellularized Skeletal Muscle With Minced Muscle Autograftsmentioning

confidence: 99%

Codelivery of Infusion Decellularized Skeletal Muscle with Minced Muscle Autografts Improved Recovery from Volumetric Muscle Loss Injury in a Rat Model

Kasukonis

Kim

Brown

et al. 2016

Tissue Engineering Part A

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Skeletal muscle is capable of robust self-repair following mild trauma, yet in cases of traumatic volumetric muscle loss (VML), where more than 20% of a muscle's mass is lost, this capacity is overwhelmed. Current autogenic whole muscle transfer techniques are imperfect, which has motivated the exploration of implantable scaffolding strategies. In this study, the use of an allogeneic decellularized skeletal muscle (DSM) scaffold with and without the addition of minced muscle (MM) autograft tissue was explored as a repair strategy using a lower-limb VML injury model (n = 8/sample group). We found that the repair of VML injuries using DSM + MM scaffolds significantly increased recovery of peak contractile force (81 -3% of normal contralateral muscle) compared to unrepaired VML controls (62 -4%). Similar significant improvements were measured for restoration of muscle mass (88 -3%) in response to DSM + MM repair compared to unrepaired VML controls (79 -3%). Histological findings revealed a marked decrease in collagen dense repair tissue formation both at and away from the implant site for DSM + MM repaired muscles. The addition of MM to DSM significantly increased MyoD expression, compared to isolated DSM treatment (21-fold increase) and unrepaired VML (37-fold) controls. These findings support the further exploration of both DSM and MM as promising strategies for the repair of VML injury.

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“…Apart from biochemical methods, biophysical strategies have recently been proposed to improve the efficiency of cell and lineage reprogramming131415. This strategy utilises cell-surface interactions, a well-defined and stable method, to modulate the cellular response.…”

mentioning

confidence: 99%

Binary colloidal crystals (BCCs) as a feeder-free system to generate human induced pluripotent stem cells (hiPSCs)

Wang

Hung

Thissen

et al. 2016

Sci Rep

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Human induced pluripotent stem cells (hiPSCs) are capable of differentiating into any cell type and provide significant advances to cell therapy and regenerative medicine. However, the current protocol for hiPSC generation is relatively inefficient and often results in many partially reprogrammed colonies, which increases the cost and reduces the applicability of hiPSCs. Biophysical stimulation, in particular from tuning cell-surface interactions, can trigger specific cellular responses that could in turn promote the reprogramming process. In this study, human fibroblasts were reprogrammed into hiPSCs using a feeder-free system and episomal vectors using novel substrates based on binary colloidal crystals (BCCs). BCCs are made from two different spherical particle materials (Si and PMMA) ranging in size from nanometers to micrometers that self-assemble into hexagonal close-packed arrays. Our results show that the BCCs, particularly those made from a crystal of 2 μm Si and 0.11 μm PMMA particles (2SiPM) facilitate the reprogramming process and increase the proportion of fully reprogrammed hiPSC colonies, even without a vitronectin coating. Subsequent isolation of clonal hiPSC lines demonstrates that they express pluripotent markers (OCT4 and TRA-1-60). This proof-of-concept study demonstrates that cell reprogramming can be improved on substrates where surface properties are tailored to the application.

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Enhanced efficiency of genetic programming toward cardiomyocyte creation through topographical cues

Cited by 86 publications

References 61 publications

The characterization of decellularized human skeletal muscle as a blueprint for mimetic scaffolds

The characterization of decellularized human skeletal muscle as a blueprint for mimetic scaffolds

Codelivery of Infusion Decellularized Skeletal Muscle with Minced Muscle Autografts Improved Recovery from Volumetric Muscle Loss Injury in a Rat Model

Binary colloidal crystals (BCCs) as a feeder-free system to generate human induced pluripotent stem cells (hiPSCs)

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