Enhanced electrochemiluminescence biosensing of gene-specific methylation in thyroid cancer patients' plasma based integrated graphitic carbon nitride-encapsulated metal-organic framework nanozyme optimized by central composite design

Khoshfetrat, Seyyed Mehdi; Dorraji, Parisa Seyed; Fotouhi, Lida; Hosseini, Mohammad Javad; Khatami, Fatemeh; Moazami, Hamid Reza; Omidfar, Kobra

doi:10.1016/j.snb.2022.131895

Cited by 35 publications

(9 citation statements)

References 44 publications

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“…Notably, herein, a combination of the following factors can simultaneously achieve target recognition and multiple signal amplification: (i) dual-aptamer (Tro4 and Tro6) in the format of the sandwich form, leading to the recognition of the cTnI specifically; (ii) CC has a high electrocatalytic activity that can be used to accelerate electron transfer toward the electro-oxidation of hydrazine by lowering the overvoltage; (iii) AuNPs have a high electrical conductivity, which can increase the aptasensor’s sensitivity; (iv) High porosity of NH 2 -MIL-53(Fe), which can improve CC loading capacity and promote electron transfer; (v) Effectively increases the sensitivity from the benefits of the synergistic effect of the Au/MIL-53(Fe) and CC; (vi) MNPs reduced background current , while increasing the signal-to-noise ratio ( S / N ) in complex human plasma, where cTnI coexists with other proteins that may significantly interfere with the electrochemical signal. To optimize different parameters, a multivariate method was applied, which provided advantages as opposed to the one-variable-at-a-time (OVAT) approach, such as fewer trials, simpler data evaluation, and investigation of the relationships between factors (for a detailed discussion about optimization of cTnI, see Supporting Information, Section S10, and related Tables S1, S2, and Figure S1). An iron-based MOF (Materiaux Institut Lavoisier MOF 53) formed by the integration of hexagonal FeO 6 chains with a 2-aminoterephthalic acid dicarboxylate anion was used as a signaling agent, linking AuNPs, aptamers, and CC platforms.…”

Section: Resultsmentioning

confidence: 99%

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Electrochemical Sandwich-Type Aptasensor Based on the Multifunctional Catechol-Loaded Au/MIL-53(Fe) for Detection of Cardiac Troponin I

Momeni,

Khoshfetrat,

Zarei

2023

ACS Appl. Nano Mater.

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Accurate detection of irreversible acute myocardial infarction (AMI) is always a challenge in clinical emergencies, and its early diagnosis can increase the patient's chance of surviving. Herein, an electrochemical aptasensor with excellent sensitivity and accuracy was developed for the detection of cardiac troponin I (cTnI). This aptasensor is prepared using sandwiching the cTnI biomarker between the magnetic nanoparticles/Tro6 aptamer (MNPs/Tro6) and multifunctional catechol-(CC)-loaded Tro4/ Au/MIL-53(Fe)-based nanocomposite [Tro4/CC/Au/MIL-53(Fe)]. The CC/Au/MIL-53(Fe) provides high electrocatalysis toward electro-oxidation of hydrazine, numerous active sites as the signal amplifier and Tro4 aptamer carrier, on the one hand, and the MNPs, which provide the high complex media's signal-to-noise ratio (S/N), on the other hand. Analysis of the electrochemical impedance spectroscopy data revealed that the double layer capacitance (surface roughness effect) contributed to the CC/Au/MIL-53(Fe)'s increased activity in part but that the intrinsic activity was mostly responsible (synergistic effect). The optimized sandwich-type aptasensor by a face-centered central composite design allows for the detection of cTnI (0.5 pg mL −1 ) with a broad dynamic range (2 pg mL −1 to 150 ng mL −1 ), addressing the clinical necessity of AMI diagnosis as well as exceptional selectivity among different biomarkers. The recommended method was effectively used to assess cTnI in AMI patient plasma, above 90% clinical sensitivity, highlighting the capability of the platform for bioanalysis in real-world samples.

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Section: Resultsmentioning

confidence: 99%

“…Detection of cTnI. The carboxylate-modified MNPs and aminated Tro6 were synthesized in accordance with our previously described study 39 (details are provided in the Supporting Information, Sections S3 and S4).…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Electrochemical Sandwich-Type Aptasensor Based on the Multifunctional Catechol-Loaded Au/MIL-53(Fe) for Detection of Cardiac Troponin I

Momeni,

Khoshfetrat,

Zarei

2023

ACS Appl. Nano Mater.

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“…MNP-based electrochemical biosensors have also shown potential in the sensitive detection of DNA methylation, which is crucial in various biomedical applications ( Parihar and Khan, 2023 ). Khoshfetrat et al (2022) designed a magnetic nano biosensor for DNA methylation analysis by combining it with a highly sensitive electrochemiluminescence immuno-DNA sensor. This sensor utilized a sandwiching approach, where the target methylated DNA was placed between MNPs and an anti-5-methylcytosine monoclonal antibody (MNPs/anti-5mc) and a phosphorylated DNA capture probe.…”

Section: Application Of Mnps-based Biosensor For Poctmentioning

confidence: 99%

Advancements in magnetic nanoparticle-based biosensors for point-of-care testing

Wang,

Jin,

Hang-Mei Leung

et al. 2024

Front. Bioeng. Biotechnol.

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The significance of point-of-care testing (POCT) in early clinical diagnosis and personalized patient care is increasingly recognized as a crucial tool in reducing disease outbreaks and improving patient survival rates. Within the realm of POCT, biosensors utilizing magnetic nanoparticles (MNPs) have emerged as a subject of substantial interest. This review aims to provide a comprehensive evaluation of the current landscape of POCT, emphasizing its growing significance within clinical practice. Subsequently, the current status of the combination of MNPs in the Biological detection has been presented. Furthermore, it delves into the specific domain of MNP-based biosensors, assessing their potential impact on POCT. By combining existing research and spotlighting pivotal discoveries, this review enhances our comprehension of the advancements and promising prospects offered by MNP-based biosensors in the context of POCT. It seeks to facilitate informed decision-making among healthcare professionals and researchers while also promoting further exploration in this promising field of study.

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“…Moreover, due to the strong electron-donating nature, this nanomaterial has a high electrocatalytic activity [ 71 ], so this property has been mainly exploited to anchor species and increase the selectivity in the design of electrochemical (bio)sensors. Table 4 summarizes the main characteristics of selected electrochemical (bio)sensors involving gC 3 N 4 [ 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 ]. A hybrid nanozyme consisting of in situ growing PtNPs on gC 3 N 4 nanosheets with enhanced peroxidase-mi-micking catalytic activity was prepared.…”

Section: Nanozymes Involving Graphitic Carbon Nitridementioning

confidence: 99%

Carbon-Based Enzyme Mimetics for Electrochemical Biosensing

Sánchez-Tirado,

Yáñez-Sedeño,

Pingarrón

2023

Micromachines

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Natural enzymes are used as special reagents for the preparation of electrochemical (bio)sensors due to their ability to catalyze processes, improving the selectivity of detection. However, some drawbacks, such as denaturation in harsh experimental conditions and their rapid de- gradation, as well as the high cost and difficulties in recycling them, restrict their practical applications. Nowadays, the use of artificial enzymes, mostly based on nanomaterials, mimicking the functions of natural products, has been growing. These so-called nanozymes present several advantages over natural enzymes, such as enhanced stability, low cost, easy production, and rapid activity. These outstanding features are responsible for their widespread use in areas such as catalysis, energy, imaging, sensing, or biomedicine. These materials can be divided into two main groups: metal and carbon-based nanozymes. The latter provides additional advantages compared to metal nanozymes, i.e., stable and tuneable activity and good biocompatibility, mimicking enzyme activities such as those of peroxidase, catalase, oxidase, superoxide dismutase, nuclease, or phosphatase. In this review article, we have focused on the use of carbon-based nanozymes for the preparation of electrochemical (bio)sensors. The main features of the most recent applications have been revised and illustrated with examples selected from the literature over the last four years (since 2020).

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Enhanced electrochemiluminescence biosensing of gene-specific methylation in thyroid cancer patients' plasma based integrated graphitic carbon nitride-encapsulated metal-organic framework nanozyme optimized by central composite design

Cited by 35 publications

References 44 publications

Electrochemical Sandwich-Type Aptasensor Based on the Multifunctional Catechol-Loaded Au/MIL-53(Fe) for Detection of Cardiac Troponin I

Electrochemical Sandwich-Type Aptasensor Based on the Multifunctional Catechol-Loaded Au/MIL-53(Fe) for Detection of Cardiac Troponin I

Advancements in magnetic nanoparticle-based biosensors for point-of-care testing

Carbon-Based Enzyme Mimetics for Electrochemical Biosensing

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