Enhanced Enzyme Activity of Thermoalkaliphilic Laccase (CtLac) of Caldalkalibacillus thermarum Strain TA2.A1 by Random and Site-directed Mutagenesis and Its Application to Lignin Degradation
Abstract:Background: Thermoalkaliphilic laccase, CtLac, has advantageous properties such as high enzyme activity and stability at 70℃ and pH 8.0 in the presence of organic solvents and surfactants, which confers a potential industrial application. Moreover, CtLac depolymerized lignin and produced high-value benzaldehyde chemicals such as vanillin. In the present study, the directed evolution approach using the combination of random and site-directed mutagenesis was performed to enhance the laccase activity of CtLac. Tw… Show more
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