Enhanced exopolysaccharide yield and antioxidant activities of Schizophyllum commune fermented products by the addition of Radix Puerariae

Abstract

To increase the production of exopolysaccharides and expand the application of Schizophyllum commune fermentation liquid, the traditional Chinese medicine Radix Puerariae with outstanding biological activity was selected as a culture additive.

Mechanism of Longevity Extension of Caenorhabditis elegans Induced by Schizophyllum commune Fermented Supernatant With Added Radix Puerariae

“…As shown in Table 1 , the total phenolics and total flavonoids of SC-RP were 3,731.56 ± 54.07 mg GAE/100 g, FW and 27.97 ± 0.41 mg CE/100 g, FW, respectively. SC-RP also contains 6.76 ± 0.47 mg/mL schizophyllan and 4.66 ± 0.05 mg/mL total protein ( 18 ). Moreover, the addition of RP introduces puerarin into the fermentation products, with a content of 56.45 ± 3.26 mg/100 g, FW.…”

“…Radix Puerariae (RP) is rich in isoflavones, which are primary polyphenolic antioxidants found in medicinal and edible plants (16,17). Our previous study have confirmed that adding RP to the S. commune fermentation system can increase the antioxidant activity of the fermented supernatant, and these antioxidant activities are mainly contributed by the puerarin from RP and some new ingredients synthesized during fermentation such as resveratrol (18). Furthermore, the above results proves the feasibility of Schizophyllum liquid fermentation system as a bioreactor and provides a reference for the biotransformation of edible medicinal fungi such as Cordyceps militaris and Ganoderma lucidum et al…”

“…# P < 0.05, ## P< 0.01, and ### P < 0.001 indicated statistical significance in different SC-RP treated groups. with the resulting fermented supernatant (SC-RP) containing a considerable phytochemical composition (18). The phenolic content of SC-RP was 8-to 18-times higher when compared with that of a blueberry extract (38), which suggests that SC-RP is a potentially abundant source of phytochemicals.…”

“…The S. commune strain was cultured initially in a seed medium as a fermented seed at 28 • C for 3 days. The seed culture was inoculated with a 10.0 % inoculum size (v/v) to fermentation medium containing 12.80 g/L RP, and cultivation was carried out on a rotary shaker (ZAZY-78BN, ZHICHU, China) at 160 rpm and 28 • C. After 7.0 d fermentation, the fermentation broth was used a centrifuge (GL-21M, CENCE, China) at 15000 g for 10 min to separate the fungi and supernatant, and the SC-RP was sterilized through a 0.22-µm filter membrane and collected for further study (18).…”

“…The antioxidant activity in vitro of fermented supernatant cultured from S. commune in RP supplemented medium was verified ( 18 ). However, whether the significant antioxidant activity of SC-RP can play an anti-aging effect remains unclear.…”