Enhanced expression of an endoglucanase in Bacillus subtilis by using the sucrose-inducible sacB promoter and improved properties of the recombinant enzyme

Liu, Sen-Lin; Du, Kun

doi:10.1016/j.pep.2012.03.015

Cited by 28 publications

(28 citation statements)

References 25 publications

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“…Eight antagonistic isolates were screened by plate assays for phosphate solubilization , indole‐3‐acetic acid (IAA) secretion indicating plant growth promotion , and siderophore production , as well as chitinase , glucanase , and protease activities. Tests were conducted at 25 °C.…”

Section: Methodsmentioning

confidence: 99%

Native soil bacteria isolates in Mexico exhibit a promising antagonistic effect against Fusarium oxysporum f. sp. radicis‐lycopersici

Cordero-Ramírez

López-Rivera

Figueroa-López

et al. 2013

J. Basic Microbiol.

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Sinaloa state accounts for 23% of Mexico's tomato production. One constraint on this important crop is the Fusarium crown and root rot, caused by Fusarium oxysporum f. sp. radicis-lycopersici, which has been reported to reduce crop yield by up to 50%. In this study, we set out to identify bacterial populations which could be used to control this disease through natural antagonism. Five tomato rhizospheric soil samples were collected, dried for 1-week, and homogenized. Sub-samples were used to prepare an aqueous solution used to isolate microorganisms in pure cultures. Organisms were purified and grown separately, and used to generate a collection of 705 bacterial isolates. Thirty-four percent from this bank (254 strains) was screened against Forl, finding 27 bacteria displaying in vitro Forl growth inhibition levels from 5% to 60%. These isolates belonged to the genus Bacillus and their 16Sr DNA sequences showed that they are closely related to seven species and they were putatively designated as: B. subtilis, B. cereus, B. amyloliquefaciens, B. licheniformis, B. thuringiensis, B. megaterium, and B. pumilus. One isolate belonged to the genus Acinetobacter. Two B. subtilis isolates (144 and 151) and one B. cereus isolate (171) showed the best antagonistic potential against FCRRT when evaluated on seedlings. Plate and activity assays indicate that these isolates include a diverse repertoire of functional antagonistic traits that might explain their ability to control FCRRT. Moreover, bacteria showed partial hemolytic activity, and future research will be directed at ensuring that their application will be not harmful for humans and effective against Forl in greenhouse or field conditions.

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Section: Methodsmentioning

confidence: 99%

Native soil bacteria isolates in Mexico exhibit a promising antagonistic effect against Fusarium oxysporum f. sp. radicis‐lycopersici

Cordero-Ramírez

López-Rivera

Figueroa-López

et al. 2013

J. Basic Microbiol.

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“…An alternative strategy involves a heterologous expression system . Inducible or autoinducible promoters to increase the heterologous expression level was well documented in a Bacillus model strain . Recently, coexpression of many paired class I/II diterpene synthases from ten plant species in bacterial hosts enabled the stereoselective formation of more than 50 labdane‐ and clerodane‐type skeletons .…”

Section: Biological Synthetic and Semisynthetic Approachesmentioning

confidence: 99%

“…205 Inducible or autoinducible promoters to increase the heterologous expression level was well documented in a Bacillus model strain. 206,207 Recently, coexpression of many paired class I/II diterpene synthases from ten plant species in bacterial hosts enabled the stereoselective formation of more than 50 labdane-and clerodane-type skeletons. 208 There has been some success in the heterologous expression of fungal gene clusters in other fungi, especially with Aspergillus nidulans as a heterologous expression system.…”

Section: Promoter Exchange and Heterologous Expressionmentioning

confidence: 99%

Strategies to diversify natural products for drug discovery

Lou

2017

Medicinal Research Reviews

222

130

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Natural product libraries contain specialized metabolites derived from plants, animals, and microorganisms that play a pivotal role in drug discovery due to their immense structural diversity and wide variety of biological activities. The strategies to greatly extend natural product scaffolds through available biological and chemical approaches offer unique opportunities to access a new series of natural product analogues, enabling the construction of diverse natural product-like libraries. The affordability of these structurally diverse molecules has been a crucial step in accelerating drug discovery. This review provides an overview of various approaches to exploit the diversity of compounds for natural product-based drug development, drawing upon a series of examples to illustrate each strategy.

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“…For example, the heterologous expression of an alkaline cellulase in a cellulolytic Bacillus strain was achieved under control of a sucrose inducible sacB promoter via the use of a rich growth medium containing yeast extract, tryptone and glucose. Using this system, the expression level of the heterologous cellulase was raised 20-fold, as compared with expression under its own promoter [ 30 ]. In another study, a β-galactosidase was expressed in a Bacillus strain under control of an artificial stationary phase specific, auto-inducible promoter, based on an engineered cry3Aa promoter sequence [ 31 ], which may also be applicable for cellulase expression.…”

Section: Bacterial Expression Systemsmentioning

confidence: 99%

“…However, over the past few years, increasing understanding of the problems faced has led to significant progress in the expression of both single cellulase enzymes and in the creation of cellulase mixtures and cellulosomes in bacteria. There are several reports where a homologous set of cellulases, such as those in B. subtilis , have been supplemented by the introduction of heterologous cellulase-coding sequences [ 30 , 80 , 81 ]. Additionally, non-natively cellulolytic bacteria, like E. coli and Z. mobilis have been transformed into cellulolytic bacteria by genetic manipulation, in order to achieve production of special metabolic products using cellulosic substrates as their carbon source [ 82 , 83 ] or to enhance the properties of probiotic microorganisms [ 84 ].…”

Section: Bacterial Expression Systemsmentioning

confidence: 99%

Challenges and advances in the heterologous expression of cellulolytic enzymes: a review

Lambertz

Garvey

Klinger

et al. 2014

Biotechnol Biofuels

167

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Second generation biofuel development is increasingly reliant on the recombinant expression of cellulases. Designing or identifying successful expression systems is thus of preeminent importance to industrial progress in the field. Recombinant production of cellulases has been performed using a wide range of expression systems in bacteria, yeasts and plants. In a number of these systems, particularly when using bacteria and plants, significant challenges have been experienced in expressing full-length proteins or proteins at high yield. Further difficulties have been encountered in designing recombinant systems for surface-display of cellulases and for use in consolidated bioprocessing in bacteria and yeast. For establishing cellulase expression in plants, various strategies are utilized to overcome problems, such as the auto-hydrolysis of developing plant cell walls. In this review, we investigate the major challenges, as well as the major advances made to date in the recombinant expression of cellulases across the commonly used bacterial, plant and yeast systems. We review some of the critical aspects to be considered for industrial-scale cellulase production.

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Enhanced expression of an endoglucanase in Bacillus subtilis by using the sucrose-inducible sacB promoter and improved properties of the recombinant enzyme

Cited by 28 publications

References 25 publications

Native soil bacteria isolates in Mexico exhibit a promising antagonistic effect against Fusarium oxysporum f. sp. radicis‐lycopersici

Native soil bacteria isolates in Mexico exhibit a promising antagonistic effect against Fusarium oxysporum f. sp. radicis‐lycopersici

Strategies to diversify natural products for drug discovery

Challenges and advances in the heterologous expression of cellulolytic enzymes: a review

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