Enhanced expression of EsWAX1 improves drought tolerance with increased accumulation of cuticular wax and ascorbic acid in transgenic Arabidopsis

Zhu, Lin; Guo, Jiansheng; Zhu, Jian Kang; Zhou, Cheng

doi:10.1016/j.plaphy.2013.11.028

Cited by 83 publications

(58 citation statements)

References 40 publications

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“…abscisic acid) in plants grown on soil with a low water potential (Davies and Zhang, 1991;Li et al, 2000;Schroeder et al, 2001;Wilkinson and Davies, 2002;Chaves et al, 2009;Pinheiro and Chaves, 2011) or by the CO2 concentration in the leaf (Vavasseur and Raghavendra, 2005) is well known. Besides the reversible regulation of stomatal opening, transpiration and leaf temperature may be affected by the deposition of cuticular waxes or by the formation of stomatal plugs in a less reversible or even irreversible manner (Stockey and Ko, 1986;Kozlowski and Pallardy, 2002;Gallé and Feller, 2007;Zhu et al, 2014). The enhanced deposition of cuticular waxes was found to be correlated with an improved drought tolerance (Aharoni et al, 2004;Yang et al, 2011).…”

Section: Photosynthetically Active Leaf Areamentioning

confidence: 99%

Drought stress and carbon assimilation in a warming climate: Reversible and irreversible impacts

Feller

2016

Journal of Plant Physiology

122

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Global change is characterized by increased CO2 concentration in the atmosphere, increasing average temperature and more frequent extreme events including drought periods, heat waves and flooding. Especially the impacts of drought and of elevated temperature on carbon assimilation are considered in this review. Effects of extreme events on the subcellular level as well as on the whole plant level may be reversible, partially reversible or irreversible. The photosynthetically active biomass depends on the number and the size of mature leaves and the photosynthetic activity in this biomass during stress and subsequent recovery phases. The total area of active leaves is determined by leaf expansion and senescence, while net photosynthesis per leaf area is primarily influenced by stomatal opening (stomatal conductance), mesophyll conductance, activity of the photosynthetic apparatus (light absorption and electron transport, activity of the Calvin cycle) and CO2 release by decarboxylation reactions (photorespiration, dark respiration). Water status, stomatal opening and leaf temperature represent a "magic triangle" of three strongly interacting parameters. The response of stomata to altered environmental conditions is important for stomatal limitations. Rubisco protein is quite thermotolerant, but the enzyme becomes at elevated temperature more rapidly inactivated (decarbamylation, reversible effect) and must be reactivated by Rubisco activase (carbamylation of a lysine residue). Rubisco activase is present under two forms (encoded by separate genes or products of alternative splicing of the premRNA from one gene) and is very thermosensitive. Rubisco activase was identified as a key protein for photosynthesis at elevated temperature (non-stomatal limitation). During a moderate heat stress Rubisco activase is reversibly inactivated, but during a more severe stress (higher temperature and/or longer exposure) the protein is irreversibly inactivated, insolubilized and finally degraded. On the level of the leaf, this loss of photosynthetic activity may still be reversible when new Rubisco activase is produced by protein synthesis. Rubisco activase as well as enzymes involved in the detoxification of reactive oxygen species or in osmoregulation are considered as important targets for breeding crop plants which are still productive under drought and/or at elevated leaf temperature in a changing climate.

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Section: Photosynthetically Active Leaf Areamentioning

confidence: 99%

Drought stress and carbon assimilation in a warming climate: Reversible and irreversible impacts

Feller

2016

Journal of Plant Physiology

122

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“…The RNA was then reversely transcripted into first cDNA as templates of real-time quantitative polymerase chain reaction (qRT-PCR). The qRT-PCR reactions were performed in a 7500 real-time PCR machine (ABI, USA) according to the method described by Zhu et al (2014). The housekeeping gene ACTIN was selected as internal reference to normalize targeted gene expression.…”

Section: Gene Expression Analysismentioning

confidence: 99%

Improved iron acquisition ofAstragalus sinicusunder low iron-availability conditions by soil-borne bacteriaBurkholderia cepacia

Zhou

Zhu

et al. 2017

Journal of Plant Interactions

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Soil bacteria can assist plant growth and increase uptake of nutrient elements, the question arises as to whether beneficial soil microbes confer augmented iron (Fe) content of host plants under Fe limited conditions. Herein, a novel strain of Burkholderia cepacia (strain JFW16) was isolated from rhziospheric soils of Astragalus sinicus grown under alkaline conditions. Inoculation of plants with B. cepacia JFW16 displayed increased endogenous Fe content compared with non-inoculated plants. Growth promotion and enhanced photosynthetic capacity were also observed for the inoculated plants. The inoculation with JFW16 significantly increased the rhizospheric acidification, and up-regulated the transcription of some Fe acquisition-associated genes in Astragalus sinicus. Moreover, the metabolic pathways of flavins were remarkably enhanced in the inoculated plants, showing the increased biosynthesis and release of flavins in roots. Collectively, these findings demonstrated the potential of B. cepacia JFW16 to improve Fe assimilation in agricultural crops. ARTICLE HISTORY

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“…In these plants, the ectopic accumulation of a thick cuticle may have prevented cellular growth, or the side effects may have led to decreased plant growth. The inhibited growth caused by the overexpression of TF genes regulating cuticle formation was also observed in studies involving WAX INDUCER 1 (WIN1)/SHINE1 (SHN1) and Eutrema salsugineum WAX1 (EsWAX1), which is an ortholog of the MYB96 stress-inducible cuticle regulator (Broun et al 2004;Zhu et al 2014). However, the use of the stressinducible RD29A promoter and a chemical-inducible promoter to express EsWAX1 and WIN1/SHN1, respectively, improves drought tolerance (with no adverse effects on growth) through increased accumulation of cuticular wax and other effects not directly related to wax accumulation (Yang et al 2011;Zhu et al 2014).…”

Section: Constitutive Expression Of Mixta-like Transcription Factorsmentioning

confidence: 63%

Enhanced cuticle accumulation by employing MIXTA-like transcription factors

Oshima

Mitsuda

2016

Plant Biotechnology

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The cuticle covers almost the entire aerial surface of terrestrial plants, and provides protection from abiotic and biotic stresses. Cuticles basically consist of wax and cutin, and are produced with variable structures and thicknesses depending on the plant and organ. The application of plant cuticles to improve stress tolerance and wax production requires the deposition of the cuticle at specific times to avoid undesirable side effects. We previously showed that the MYB106 and MYB16 MIXTA-like transcription factors regulate cuticle formation. However, MYB106 over-expression results in severe dwarfism. In this study, we identified genes downstream of these MYB transcription factors and used their promoters to express MYB106 and MYB16 fused to the strong transcriptional activation domain, VP16. Comparisons of plant growth and cuticle morphology revealed that MYB106 and MYB16 preferentially produced cuticles that are typically observed in petals and leaves, respectively. Additionally, the CYP77A6 and CYP86A4 promoters effectively induced cuticle accumulation in leaves and petals, respectively, without inhibiting plant growth. Our strategy may be useful for increasing or altering cuticles in agronomically important plants.

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Enhanced expression of EsWAX1 improves drought tolerance with increased accumulation of cuticular wax and ascorbic acid in transgenic Arabidopsis

Cited by 83 publications

References 40 publications

Drought stress and carbon assimilation in a warming climate: Reversible and irreversible impacts

Drought stress and carbon assimilation in a warming climate: Reversible and irreversible impacts

Improved iron acquisition ofAstragalus sinicusunder low iron-availability conditions by soil-borne bacteriaBurkholderia cepacia

Enhanced cuticle accumulation by employing MIXTA-like transcription factors

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