Enhanced Expression of Iba1, Ionized Calcium-Binding Adapter Molecule 1, After Transient Focal Cerebral Ischemia In Rat Brain

Ito, Daisuke; Tanaka, Kortaro; Suzuki, Shigeaki; Dembo, Tomohisa; Fukuuchi, Yasuo

doi:10.1161/01.str.32.5.1208

Cited by 530 publications

(424 citation statements)

References 39 publications

Supporting

Mentioning

390

Contrasting

Order By: Relevance

“…Iba-1 is a microglia/macrophage-specific calcium-binding protein that participates in membrane ruffling and phagocytosis in activated microglia [27]. Accordingly, the activation of Iba-1 mRNA expression was observed after LPS-stimulation (Fig.…”

Section: Anti-inflammatory Activity Of Gp-ee On Lps-activated N13 Cellsmentioning

confidence: 93%

Obtaining from Grape Pomace an Enzymatic Extract with Anti-inflammatory Properties

Rodríguez-Morgado¹,

Candiracci

Santa-María³

et al. 2014

Plant Foods Hum Nutr

View full text Add to dashboard Cite

Grape pomace, a winemaking industry by-product, is a rich source of bioactive dietary compounds. Using proteases we have developed an enzymatic process for obtaining a water-soluble extract (GP-EE) that contains biomolecules such as peptides, carbohydrates, lipids and polyphenols in soluble form. Of especial interest is its high polyphenol content (12 %), of which 77 % are flavonoids and 33 % are phenolic acids. The present study evaluates in vitro the potential anti-inflammatory effect of GP-EE by monitoring the expression of inflammatory molecules on N13 microglia cells stimulated with lipopolysaccharide (LPS). GP-EE decreases the mRNA levels of the inflammatory molecules studied. The molecules under study were as follows: inducible nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), the ionized calcium binding adaptor molecule-1(Iba-1) and the Toll like receptor-4 (TLR-4), as well as the iNOS protein level in LPS-stimulated microglia. Our findings suggest that, as a result of its ability to regulate excessive microglial activation, GP-EE possesses antiinflammatory properties. Therefore, acting as a chemopreventive agent, it may be of therapeutic interest in neurodegenerative diseases involving neuroinflammation. We can, therefore, propose GP-EE as a useful natural extract and one that would be beneficial to apply in the field of functional foods.

show abstract

Section: Anti-inflammatory Activity Of Gp-ee On Lps-activated N13 Cellsmentioning

confidence: 93%

Obtaining from Grape Pomace an Enzymatic Extract with Anti-inflammatory Properties

Rodríguez-Morgado¹,

Candiracci

Santa-María³

et al. 2014

Plant Foods Hum Nutr

View full text Add to dashboard Cite

show abstract

“…27) By immunohistochemical analyses, anti-Iba1 antibody was found to specifically recognize ramified microglia in normal rat brain, and Iba1 protein was strongly upregulated in activated microglia. 28) Therefore, Iba1 may play significant roles in the regulation of some immunological and pathophysiological functions of microglia, and serve as a novel marker for detecting the activation of microglia. However, this study is the first to show that ginsenoside Rg 3 plays a modulatory role in microglia activation using an in vivo animal study.…”

Section: Effect Of Ginsenoside Rg 3 On Microglia Activation In Thementioning

confidence: 99%

Ginsenoside Rg<sub>3</sub> Attenuates Microglia Activation Following Systemic Lipopolysaccharide Treatment in Mice

Park

Choi

Sohn

et al. 2012

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

“…Expression of Iba1 is up-regulated in activated microglia following facial nerve axotomy [13], ischemia [14], and several brain diseases [15,16], thereby implicating it in the activated phenotypes of microglia. Iba 1 shares active locus with F -actin [filamentous actin] on membrane ruffles induced by macrophage colony -stimulating factor [M-CSF] and in phagocytic cups formed during zymosan uptake by microglia [17].…”

Section: Introductionmentioning

confidence: 99%

Visualisation of Microglia with the use of Immunohistochemical Double Staining Method for CD-68 and Iba-1 of Cerebral Tissue Samples in Cases of Brain Contusions

Stankov¹,

Belakaposka-Srpanova²,

Bitoljanu³

et al. 2015

Prilozi

View full text Add to dashboard Cite

In the recent years it has been confirmed that the main component of the immune response in an injury of the nerve cell comes from microglia and macrophages. The main challenge in the field of microglia research is to detect the different stages of cellular activation by visualization of the cell morphology. The existing visualization techniques are based on surface molecules expression in resting and activated microglia cells. For visualization of the microglial cells and their functional state we used double labeling method for cd-68 and iba1 in brain contusions with different survival time. Microglia are stained brown with Iba-1, whereas microglia impregnated with black, grainy color, represents activated microglia stained with CD 68. We had significantly positive results, and we were able to observe changes in the morphology of the microglia that correlated with the survival time. Using double labeling with Iba-1 and cd68 we were able to determine their physiological state based on the morphology and immunoreactivity.

show abstract

Enhanced Expression of Iba1, Ionized Calcium-Binding Adapter Molecule 1, After Transient Focal Cerebral Ischemia In Rat Brain

Cited by 530 publications

References 39 publications

Obtaining from Grape Pomace an Enzymatic Extract with Anti-inflammatory Properties

Obtaining from Grape Pomace an Enzymatic Extract with Anti-inflammatory Properties

Ginsenoside Rg<sub>3</sub> Attenuates Microglia Activation Following Systemic Lipopolysaccharide Treatment in Mice

Visualisation of Microglia with the use of Immunohistochemical Double Staining Method for CD-68 and Iba-1 of Cerebral Tissue Samples in Cases of Brain Contusions

Contact Info

Product

Resources

About